Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Rapid microbiological assay for chloramphenicol and tetracyclines.

Literature DB >> 180881

Rapid microbiological assay for chloramphenicol and tetracyclines.

T J Louie, F P Tally, J G Bartlett, S L Gorbach.

Abstract

An agar diffusion assay for chloramphenicol and tetracyclines is described, using inhibition of hemolysis by Clostridium perfringens as a biological indicator of antimicrobial activity. Well-defined zones formed after a 1.5- to 2-h incubation in an anaerobic chamber or after 3 h using a GasPak system. Serum levels of chloramphenicol as low as 2.0 mug/ml could be reproducibly determined with this method. Concurrent presence of gentamicin did not influence the assay. Levels of penicillin G were also reproducibly determined with this method, although use of stable zones of growth inhibition which would form after overnight incubation were a more convenient end point for this bactericidal drug. The hemolytic assay with C. perfringens is a reproducible, accurate, and rapid method for determination of levels for chloramphenicol and tetracyclines.

Entities: Chemical Disease Species

Mesh：

Substances：

Year: 1976 PMID： 180881 PMCID： PMC429642 DOI： 10.1128/AAC.9.6.874

Source DB: PubMed Journal: Antimicrob Agents Chemother ISSN： 0066-4804 Impact factor: 5.191

15 in total

1. Micromethod for determination of concentration of antibiotics in serum for application in clinical pediatrics.

Authors: R FUJII; M GROSSMAN; W TICKNOR
Journal: Pediatrics Date: 1961-10 Impact factor: 7.124

2. Chloramphenicol in the newborn infant. A physiologic explanation of its toxicity when given in excessive doses.

Authors: C F WEISS; A J GLAZKO; J K WESTON
Journal: N Engl J Med Date: 1960-04-21 Impact factor: 91.245

3. Effect of chloramphenicol on erythropoiesis.

Authors: P SAIDI; R O WALLERSTEIN; P M AGGELER
Journal: J Lab Clin Med Date: 1961-02

4. Antimicrobial susceptibility of Haemophilus influenzae.

Authors: C Thornsberry; L A Kirven
Journal: Antimicrob Agents Chemother Date: 1974-11 Impact factor: 5.191

5. DIFFUSION PLATE ASSAY FOR CHLORAMPHENICOL AND AUREOMYCIN.

Authors: W A Randall; A Kirshbaum; J K Nielsen; D Wintermere
Journal: J Clin Invest Date: 1949-09 Impact factor: 14.808

6. A cylinder-plate assay for chloromycetin in body fluids and tissue extracts.

Authors: D G SMITH; C B LANDERS; J FORGACS
Journal: J Lab Clin Med Date: 1950-07

7. A turbidimetric method for the assay of antibiotics.

Authors: D A JOSLYN; M GALBRAITH
Journal: J Bacteriol Date: 1950-06 Impact factor: 3.490

8. A colorimetric method for the determination of chloromycetin in serum or plasma.

Authors: S P BESSMAN; S STEVENS
Journal: J Lab Clin Med Date: 1950-01

9. Susceptibility of anaerobes to cefoxitin and other cephalosporins.

Authors: F P Tally; N V Jacobus; J G Bartlett; S L Gorbach
Journal: Antimicrob Agents Chemother Date: 1975-02 Impact factor: 5.191

10. Rapid microassays for clindamycin and gentamicin when present together and the effect of pH and of each on the antibacterial activity of the other.

Authors: L D Sabath; I Toftegaard
Journal: Antimicrob Agents Chemother Date: 1974-07 Impact factor: 5.191

15 in total

1. A competitive colorimetric chloramphenicol assay based on the non-cross-linking deaggregation of gold nanoparticles coated with a polyadenine-modified aptamer.

Authors: Yuanyang Xie; Yu Huang; Dongyun Tang; Hongliang Cui; Haiyan Cao
Journal: Mikrochim Acta Date: 2018-11-07 Impact factor: 5.833

Rapid microbiological assay for chloramphenicol and tetracyclines.

1. Micromethod for determination of concentration of antibiotics in serum for application in clinical pediatrics.

2. Chloramphenicol in the newborn infant. A physiologic explanation of its toxicity when given in excessive doses.

3. Effect of chloramphenicol on erythropoiesis.

4. Antimicrobial susceptibility of Haemophilus influenzae.

5. DIFFUSION PLATE ASSAY FOR CHLORAMPHENICOL AND AUREOMYCIN.

6. A cylinder-plate assay for chloromycetin in body fluids and tissue extracts.

7. A turbidimetric method for the assay of antibiotics.

8. A colorimetric method for the determination of chloromycetin in serum or plasma.

9. Susceptibility of anaerobes to cefoxitin and other cephalosporins.

10. Rapid microassays for clindamycin and gentamicin when present together and the effect of pH and of each on the antibacterial activity of the other.

1. A competitive colorimetric chloramphenicol assay based on the non-cross-linking deaggregation of gold nanoparticles coated with a polyadenine-modified aptamer.

2. An fMRI study of neural interaction in large-scale cortico-thalamic visual network.

3. Simple method for elimination of aminoglycosides from serum to permit bioassay of other antimicrobial agents.

4. Modification of the colorimetric assay for chloramphenicol in the presence of bilirubin.

5. High-performance liquid chromatographic assay of chloramphenicol in serum.

6. Activity of metronidazole and its hydroxy and acid metabolites against clinical isolates of anaerobic bacteria.

7. Chloramphenicol bioassay.

8. Chloramphenicol and phenobarbitone--a drug interaction.

9. Comparison of enzymatic and liquid chromatographic chloramphenicol assays.

10. Indications, interpretation and applications of antibiotic assays.