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Literature DB >> 708020

High-performance liquid chromatographic assay of chloramphenicol in serum.

Abstract

A new method for the analysis of serum chloramphenicol by reversed-phase, high-performance liquid chromatography (HPLC) is described. The method involves a preliminary extraction of 0.1 ml of serum with ethyl acetate containing an internal standard, chromatography with a reversed-phase C18 microparticulate column with an acetonitrile-acetate buffer mobile phase, and detection by measuring UV absorbance at 270 nm. Assay performance was compared with an existing microbiological assay. The HPLC method demonstrated both increased precision and increased sensitivity. The specificity of the HPLC method was also evaluated. The new method presents an alternative approach to the analysis of clinical specimens.

Entities: Chemical Gene

Mesh：

Substances：
Chloramphenicol

Year: 1978 PMID： 708020 PMCID： PMC352477 DOI： 10.1128/AAC.14.3.439

Source DB: PubMed Journal: Antimicrob Agents Chemother ISSN： 0066-4804 Impact factor: 5.191

24 in total

1. FATAL APLASTIC ANEMIA. AN EPIDEMIOLOGICAL STUDY OF ITS RELATIONSHIP TO THE DRUG CHLORAMPHENICOL.

Authors: K M SMICK; P K CONDIT; R L PROCTOR; V SUTCHER
Journal: J Chronic Dis Date: 1964-10

2. A CONTROLLED DOUBLE-BLIND STUDY OF THE HEMATOLOGIC TOXICITY OF CHLORAMPHENICOL.

Authors: J L SCOTT; S M FINEGOLD; G A BELKIN; J S LAWRENCE
Journal: N Engl J Med Date: 1965-06-03 Impact factor: 91.245

3. Chloramphenicol in the newborn infant. A physiologic explanation of its toxicity when given in excessive doses.

Authors: C F WEISS; A J GLAZKO; J K WESTON
Journal: N Engl J Med Date: 1960-04-21 Impact factor: 91.245

4. An outbreak of neonatal deaths among term infants associated with administration of chloramphenicol.

Authors: H LISCHNER; S J SELIGMAN; A KRAMMER; A H PARMELEE
Journal: J Pediatr Date: 1961-07 Impact factor: 4.406

5. Fatal circulatory collapse in premature infants receiving chloramphenicol.

Authors: L E BURNS; J E HODGMAN; A B CASS
Journal: N Engl J Med Date: 1959-12-24 Impact factor: 91.245

6. Persistence of antibiotics in blood of patients with acute renal failure. II. Chloramphenicol and its metabolic products in the blood of patients with severe renal disease or hepatic cirrhosis.

Authors: C M KUNIN; A J GLAZKO; M FINLAND
Journal: J Clin Invest Date: 1959-09 Impact factor: 14.808

7. A cylinder-plate assay for chloromycetin in body fluids and tissue extracts.

Authors: D G SMITH; C B LANDERS; J FORGACS
Journal: J Lab Clin Med Date: 1950-07

8. Reverse phase high speed liquid chromatography of antibiotics. II. Use of high efficiency small particle columns.

Authors: E R White; M A Carroll; J E Zarembo
Journal: J Antibiot (Tokyo) Date: 1977-10 Impact factor: 2.649

9. Simultaneous measurement of phenobarbital, phenytoin, primidone, ethosuximide, and carbamazepine in serum by high-pressure liquid chromatography.

Authors: P M Kabra; B E Stafford; L J Marton
Journal: Clin Chem Date: 1977-07 Impact factor: 8.327

10. CHLORAMPHENICOL IN BLOOD: SIMPLE CHEMICAL ESTIMATIONS IN PATIENTS RECEIVING MULTIPLE ANTIBIOTICS.

Authors: D W HUGHES; L K DIAMOND
Journal: Science Date: 1964-04-17 Impact factor: 47.728

11 in total

1. Modification of the colorimetric assay for chloramphenicol in the presence of bilirubin.

Authors: E O Mason; S L Kaplan; C J Baker; D A Ritter
Journal: Antimicrob Agents Chemother Date: 1979-04 Impact factor: 5.191

2. Relationship between serum and saliva chloramphenicol concentrations.

Authors: J R Koup; A H Lau; B Brodsky; R L Slaughter
Journal: Antimicrob Agents Chemother Date: 1979-05 Impact factor: 5.191

Review 3. Chloramphenicol in the 1980s.

Authors: I Shalit; M I Marks
Journal: Drugs Date: 1984-10 Impact factor: 9.546

High-performance liquid chromatographic assay of chloramphenicol in serum.

1. FATAL APLASTIC ANEMIA. AN EPIDEMIOLOGICAL STUDY OF ITS RELATIONSHIP TO THE DRUG CHLORAMPHENICOL.

2. A CONTROLLED DOUBLE-BLIND STUDY OF THE HEMATOLOGIC TOXICITY OF CHLORAMPHENICOL.

3. Chloramphenicol in the newborn infant. A physiologic explanation of its toxicity when given in excessive doses.

4. An outbreak of neonatal deaths among term infants associated with administration of chloramphenicol.

5. Fatal circulatory collapse in premature infants receiving chloramphenicol.

6. Persistence of antibiotics in blood of patients with acute renal failure. II. Chloramphenicol and its metabolic products in the blood of patients with severe renal disease or hepatic cirrhosis.

7. A cylinder-plate assay for chloromycetin in body fluids and tissue extracts.

8. Reverse phase high speed liquid chromatography of antibiotics. II. Use of high efficiency small particle columns.

9. Simultaneous measurement of phenobarbital, phenytoin, primidone, ethosuximide, and carbamazepine in serum by high-pressure liquid chromatography.

10. CHLORAMPHENICOL IN BLOOD: SIMPLE CHEMICAL ESTIMATIONS IN PATIENTS RECEIVING MULTIPLE ANTIBIOTICS.

1. Modification of the colorimetric assay for chloramphenicol in the presence of bilirubin.

2. Relationship between serum and saliva chloramphenicol concentrations.

Review 3. Chloramphenicol in the 1980s.

4. Evaluation of new blood culture processing systems.

5. Chloramphenicol bioassay.

Review 6. Clinical pharmacokinetics of chloramphenicol and chloramphenicol succinate.

7. Comparison of cefamandole, cephalothin, ampicillin, and chloramphenicol in experimental Escherichia coli meningitis.

8. Chloramphenicol pharmacokinetics in hospitalized patients.

9. High-pressure liquid chromatographic assay of chloramphenicol in biological fluids.

Review 10. Serum level monitoring of antibacterial drugs. A review.