Literature DB >> 18044863

Passive water-lipid peptide translocators with conformational switches: from single-molecule probe to cellular assay.

Ariel Fernández1, Alejandro Crespo, Axel Blau.   

Abstract

Peptide design for unassisted passive water-lipid translocation remains a challenge, notwithstanding its importance for drug delivery. We introduce a design paradigm based on conformational switches operating as passive translocation vehicles. The interfacial behavior of the molecular prototype, probed in single-molecule AFM experiments, reveals a near-barrierless translocation. The associated free-energy agrees with mesoscopic measurements, and the in vitro behavior is quantitatively reproduced in cellular assays. The prototypes herald the advent of novel nanobiomaterials for passive translocation.

Entities:  

Mesh：

Substances：

Year:  2007        PMID： 18044863      PMCID： PMC2528833          DOI： 10.1021/jp074479u

Source DB:  PubMed          Journal:  J Phys Chem B        ISSN： 1520-5207            Impact factor:   2.991


  16 in total

1.  The alpha-helix folds on the millisecond time scale.

Authors:  D T Clarke; A J Doig; B J Stapley; G R Jones
Journal:  Proc Natl Acad Sci U S A       Date:  1999-06-22       Impact factor: 11.205

2.  Unfolding pathways of individual bacteriorhodopsins.

Authors:  F Oesterhelt; D Oesterhelt; M Pfeiffer; A Engel; H E Gaub; D J Müller
Journal:  Science       Date:  2000-04-07       Impact factor: 47.728

3.  Circular dichroism spectra of short, fixed-nucleus alanine helices.

Authors:  Der-Hang Chin; Robert W Woody; Carol A Rohl; Robert L Baldwin
Journal:  Proc Natl Acad Sci U S A       Date:  2002-11-11       Impact factor: 11.205

4.  Cell-penetrating peptides. A reevaluation of the mechanism of cellular uptake.

Authors:  Jean Philippe Richard; Kamran Melikov; Eric Vives; Corinne Ramos; Birgit Verbeure; Mike J Gait; Leonid V Chernomordik; Bernard Lebleu
Journal:  J Biol Chem       Date:  2002-10-30       Impact factor: 5.157

5.  Equilibrium information from nonequilibrium measurements in an experimental test of Jarzynski's equality.

Authors:  Jan Liphardt; Sophie Dumont; Steven B Smith; Ignacio Tinoco; Carlos Bustamante
Journal:  Science       Date:  2002-06-07       Impact factor: 47.728

6.  Translocation of human calcitonin in respiratory nasal epithelium is associated with self-assembly in lipid membrane.

Authors:  M C Schmidt; B Rothen-Rutishauser; B Rist; A Beck-Sickinger; H Wunderli-Allenspach; W Rubas; W Sadée; H P Merkle
Journal:  Biochemistry       Date:  1998-11-24       Impact factor: 3.162

7.  The hydration structure of guanidinium and thiocyanate ions: implications for protein stability in aqueous solution.

Authors:  P E Mason; G W Neilson; C E Dempsey; A C Barnes; J M Cruickshank
Journal:  Proc Natl Acad Sci U S A       Date:  2003-04-08       Impact factor: 11.205

8.  NMR solution structure of the human prion protein.

Authors:  R Zahn; A Liu; T Lührs; R Riek; C von Schroetter; F López García; M Billeter; L Calzolai; G Wider; K Wüthrich
Journal:  Proc Natl Acad Sci U S A       Date:  2000-01-04       Impact factor: 11.205

9.  Critical role of a thiolate-quinone charge transfer complex and its adduct form in de novo disulfide bond generation by DsbB.

Authors:  Kenji Inaba; Yoh-hei Takahashi; Koreaki Ito; Shigehiko Hayashi
Journal:  Proc Natl Acad Sci U S A       Date:  2005-12-29       Impact factor: 11.205

10.  Sequence-dependent mechanics of single DNA molecules.

Authors:  M Rief; H Clausen-Schaumann; H E Gaub
Journal:  Nat Struct Biol       Date:  1999-04
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.