Literature DB >> 18032627

Rapid and reliable diagnostic algorithm for detection of Clostridium difficile.

Lukas Fenner1, Andreas F Widmer, Gisela Goy, Sonja Rudin, Reno Frei.   

Abstract

We evaluated a two-step algorithm for detection of Clostridium difficile in 1,468 stool specimens. First, specimens were screened by an immunoassay for C. difficile glutamate dehydrogenase antigen (C.DIFF CHEK-60). Second, screen-positive specimens underwent toxin testing by a rapid toxin A/B assay (TOX A/B QUIK CHEK); toxin-negative specimens were subjected to stool culture. This algorithm allowed final results for 92% of specimens with a turnaround time of 4 h.

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Year:  2007        PMID： 18032627      PMCID： PMC2224300          DOI： 10.1128/JCM.01503-07

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  13 in total

1.  Prospective multicenter evaluation of a new immunoassay and real-time PCR for rapid diagnosis of Clostridium difficile-associated diarrhea in hospitalized patients.

Authors:  Renate J van den Berg; Lesla S Bruijnesteijn van Coppenraet; Hendrik-Jan Gerritsen; Hubert P Endtz; Eric R van der Vorm; Ed J Kuijper
Journal:  J Clin Microbiol       Date:  2005-10       Impact factor: 5.948

2.  An epidemic, toxin gene-variant strain of Clostridium difficile.

Authors:  L Clifford McDonald; George E Killgore; Angela Thompson; Robert C Owens; Sophia V Kazakova; Susan P Sambol; Stuart Johnson; Dale N Gerding
Journal:  N Engl J Med       Date:  2005-12-01       Impact factor: 91.245

3.  Effective detection of toxigenic Clostridium difficile by a two-step algorithm including tests for antigen and cytotoxin.

Authors:  John R Ticehurst; Deborah Z Aird; Lisa M Dam; Anita P Borek; John T Hargrove; Karen C Carroll
Journal:  J Clin Microbiol       Date:  2006-03       Impact factor: 5.948

4.  Evaluation of methods for detection of toxins in specimens of feces submitted for diagnosis of Clostridium difficile-associated diarrhea.

Authors:  D O'Connor; P Hynes; M Cormican; E Collins; G Corbett-Feeney; M Cassidy
Journal:  J Clin Microbiol       Date:  2001-08       Impact factor: 5.948

Review 5.  Clostridium difficile infection.

Authors:  C P Kelly; J T LaMont
Journal:  Annu Rev Med       Date:  1998       Impact factor: 13.739

6.  Six rapid tests for direct detection of Clostridium difficile and its toxins in fecal samples compared with the fibroblast cytotoxicity assay.

Authors:  David K Turgeon; Thomas J Novicki; John Quick; LaDonna Carlson; Pat Miller; Bruce Ulness; Anne Cent; Rhoda Ashley; Ann Larson; Marie Coyle; Ajit P Limaye; Brad T Cookson; Thomas R Fritsche
Journal:  J Clin Microbiol       Date:  2003-02       Impact factor: 5.948

7.  Yield of stool culture with isolate toxin testing versus a two-step algorithm including stool toxin testing for detection of toxigenic Clostridium difficile.

Authors:  Megan E Reller; Clara A Lema; Trish M Perl; Mian Cai; Tracy L Ross; Kathleen A Speck; Karen C Carroll
Journal:  J Clin Microbiol       Date:  2007-09-05       Impact factor: 5.948

8.  Performance of the TechLab C. DIFF CHEK-60 enzyme immunoassay (EIA) in combination with the C. difficile Tox A/B II EIA kit, the Triage C. difficile panel immunoassay, and a cytotoxin assay for diagnosis of Clostridium difficile-associated diarrhea.

Authors:  Heather Snell; Meredith Ramos; Sue Longo; Michael John; Zafar Hussain
Journal:  J Clin Microbiol       Date:  2004-10       Impact factor: 5.948

9.  A European survey of diagnostic methods and testing protocols for Clostridium difficile.

Authors:  F Barbut; M Delmée; J S Brazier; J C Petit; I R Poxton; M Rupnik; V Lalande; C Schneider; P Mastrantonio; R Alonso; E Kuipjer; M Tvede
Journal:  Clin Microbiol Infect       Date:  2003-10       Impact factor: 8.067

10.  Multicenter evaluation of a new screening test that detects Clostridium difficile in fecal specimens.

Authors:  L Zheng; S F Keller; D M Lyerly; R J Carman; C W Genheimer; C A Gleaves; S J Kohlhepp; S Young; S Perez; K Ye
Journal:  J Clin Microbiol       Date:  2004-08       Impact factor: 5.948
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  43 in total

Review 1.  Review of medical and surgical management of Clostridium difficile infection.

Authors:  B Faris; A Blackmore; N Haboubi
Journal:  Tech Coloproctol       Date:  2010-05-08       Impact factor: 3.781

2.  Laboratory diagnosis of Clostridium difficile infection.

Authors:  S D Goldenberg; P R Cliff; G L French
Journal:  J Clin Microbiol       Date:  2010-08       Impact factor: 5.948

3.  Diagnostic algorithm using a sensitive broth culture method for detection of Clostridium difficile toxin from stool samples.

Authors:  Paul Bayardelle
Journal:  Can J Infect Dis Med Microbiol       Date:  2009       Impact factor: 2.471

4.  Clostridioides difficile laboratory diagnostic techniques: a comparative approach of rapid and molecular methods.

Authors:  Thais Simões Camargo; Moacyr Silva Junior; Luis Fernando Aranha Camargo; Veronica Pivetta Biotto; André Mario Doi; Paula Celia Mariko Koga; Carolina Nunes França; Marines Dalla Valle Martino
Journal:  Arch Microbiol       Date:  2021-01-18       Impact factor: 2.552

5.  Evaluation of diagnostic tests for Clostridium difficile infection.

Authors:  Jonathan Swindells; Nigel Brenwald; Nathan Reading; Beryl Oppenheim
Journal:  J Clin Microbiol       Date:  2009-12-23       Impact factor: 5.948

6.  Real-time cellular analysis coupled with a specimen enrichment accurately detects and quantifies Clostridium difficile toxins in stool.

Authors:  Bin Huang; Dazhi Jin; Jing Zhang; Janet Y Sun; Xiaobo Wang; Jeffrey Stiles; Xiao Xu; Mini Kamboj; N Esther Babady; Yi-Wei Tang
Journal:  J Clin Microbiol       Date:  2014-01-22       Impact factor: 5.948

7.  Combination of culture, antigen and toxin detection, and cytotoxin neutralization assay for optimal Clostridium difficile diagnostic testing.

Authors:  Michelle J Alfa; Shadi Sepehri
Journal:  Can J Infect Dis Med Microbiol       Date:  2013       Impact factor: 2.471

8.  A cost-effective approach for detection of toxigenic Clostridium difficile: toxigenic culture using ChromID Clostridium difficile agar.

Authors:  Shik Luk; Wing Kin To; Tak Keung Ng; Wai Ting Hui; Wing Keung Lee; Florence Lau; Almond Man Wai Ching
Journal:  J Clin Microbiol       Date:  2013-12-11       Impact factor: 5.948

9.  Comparison of a commercial real-time PCR assay for tcdB detection to a cell culture cytotoxicity assay and toxigenic culture for direct detection of toxin-producing Clostridium difficile in clinical samples.

Authors:  Paul D Stamper; Romina Alcabasa; Deborah Aird; Wisal Babiker; Jennifer Wehrlin; Ijeoma Ikpeama; Karen C Carroll
Journal:  J Clin Microbiol       Date:  2008-12-10       Impact factor: 5.948

10.  Epidemiology of Clostridium difficile-associated disease at University Hospital Basel including molecular characterisation of the isolates 2006-2007.

Authors:  L Fenner; R Frei; M Gregory; M Dangel; A Stranden; A F Widmer
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2008-06-17       Impact factor: 3.267
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