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Literature DB >> 17999150

Isotope labeling of mammalian GPCRs in HEK293 cells and characterization of the C-terminus of bovine rhodopsin by high resolution liquid NMR spectroscopy.

Karla Werner¹, Christian Richter, Judith Klein-Seetharaman, Harald Schwalbe.

Abstract

High amino acid coverage labeling of the mammalian G protein coupled receptors (GPCR) rhodopsin was established with 15N and 15N/13C isotopes. Rhodopsin was expressed at preparative scale in HEK293S cells and studied in full-length by NMR spectroscopy in detergent micelle solution. This resulted in the assignment and detailed study of the dynamic properties of the C-terminus of rhodopsin. The rhodopsin C-terminus is immobilized until Ala333, after which it becomes unstructured.

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Year: 2007 PMID： 17999150 DOI： 10.1007/s10858-007-9205-3

Source DB: PubMed Journal: J Biomol NMR ISSN： 0925-2738 Impact factor: 2.835

22 in total

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5. The retinal conformation and its environment in rhodopsin in light of a new 2.2 A crystal structure.

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Authors: Philip J Reeves; Jong-Myoung Kim; H Gobind Khorana
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Authors: J Klein-Seetharaman; P J Reeves; M C Loewen; E V Getmanova; J Chung; H Schwalbe; P E Wright; H G Khorana
Journal: Proc Natl Acad Sci U S A Date: 2002-03-19 Impact factor: 11.205

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