Literature DB >> 17977995

Rapid diagnosis of human brucellosis by quantitative real-time PCR: a case report of brucellar spondylitis.

Amalia Navarro-Martínez1, Elena Navarro, Maria Jesús Castaño, Javier Solera.   

Abstract

Blood samples and a bone biopsy specimen from one patient diagnosed with spondylodiscitis of unknown etiology were analyzed by quantitative real-time PCR to detect Brucella melitensis. The high sensitivity and specificity of this assay allowed the diagnosis of brucellar spondylitis within 24 h, a result that we were unable to obtain through the use of conventional methods.

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Year:  2007        PMID： 17977995      PMCID： PMC2224288          DOI： 10.1128/JCM.01303-07

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  13 in total

Review 1.  Quantitative PCR.

Authors:  R Jung; K Soondrum; M Neumaier
Journal:  Clin Chem Lab Med       Date:  2000-09       Impact factor: 3.694

Review 2.  Real-time PCR in virology.

Authors:  Ian M Mackay; Katherine E Arden; Andreas Nitsche
Journal:  Nucleic Acids Res       Date:  2002-03-15       Impact factor: 16.971

Review 3.  An overview of real-time quantitative PCR: applications to quantify cytokine gene expression.

Authors:  A Giulietti; L Overbergh; D Valckx; B Decallonne; R Bouillon; C Mathieu
Journal:  Methods       Date:  2001-12       Impact factor: 3.608

4.  Rapid diagnosis of Brucella bacteremia by using the BACTEC 9240 system.

Authors:  R M Bannatyne; M C Jackson; Z Memish
Journal:  J Clin Microbiol       Date:  1997-10       Impact factor: 5.948

Review 5.  Brucellar spondylitis: review of 35 cases and literature survey.

Authors:  J Solera; E Lozano; E Martínez-Alfaro; A Espinosa; M L Castillejos; L Abad
Journal:  Clin Infect Dis       Date:  1999-12       Impact factor: 9.079

Review 6.  An overview of human brucellosis.

Authors:  E J Young
Journal:  Clin Infect Dis       Date:  1995-08       Impact factor: 9.079

7.  Multivariate model for predicting relapse in human brucellosis.

Authors:  J Solera; E Martínez-Alfaro; A Espinosa; M L Castillejos; P Geijo; M Rodríguez-Zapata
Journal:  J Infect       Date:  1998-01       Impact factor: 6.072

Review 8.  Recognition and optimum treatment of brucellosis.

Authors:  J Solera; E Martínez-Alfaro; A Espinosa
Journal:  Drugs       Date:  1997-02       Impact factor: 9.546

9.  Use of real-time quantitative polymerase chain reaction to monitor the evolution of Brucella melitensis DNA load during therapy and post-therapy follow-up in patients with brucellosis.

Authors:  Elena Navarro; Juan Carlos Segura; María Jesús Castaño; Javier Solera
Journal:  Clin Infect Dis       Date:  2006-03-31       Impact factor: 9.079

10.  [A comparative therapeutic and multicenter trial of rifampicin and doxycycline versus streptomycin and doxycycline in human brucellosis].

Authors:  J Solera; F Medrano; M Rodríguez; P Geijo; J Paulino
Journal:  Med Clin (Barc)       Date:  1991-05-04       Impact factor: 1.725
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  7 in total

1.  Optimization and validation of a real-time polymerase chain reaction protocol for the diagnosis of human brucellosis.

Authors:  Hasan Zeybek; Ziya Cibali Acikgoz; Tuba Dal; Rıza Durmaz
Journal:  Folia Microbiol (Praha)       Date:  2019-07-31       Impact factor: 2.099

2.  Challenges of establishing the correct diagnosis of outbreaks of acute febrile illnesses in Africa: the case of a likely Brucella outbreak among nomadic pastoralists, northeast Kenya, March-July 2005.

Authors:  Mary D Ari; Argata Guracha; Moustafa Abdel Fadeel; Charles Njuguna; M Kariuki Njenga; Rosalia Kalani; Hassan Abdi; Osman Warfu; Victor Omballa; Christopher Tetteh; Robert F Breiman; Guillermo Pimentel; Daniel R Feikin
Journal:  Am J Trop Med Hyg       Date:  2011-11       Impact factor: 2.345

Review 3.  Laboratory Diagnosis of Human Brucellosis.

Authors:  Pablo Yagupsky; Pilar Morata; Juan D Colmenero
Journal:  Clin Microbiol Rev       Date:  2019-11-13       Impact factor: 26.132

4.  Joint EANM/ESNR and ESCMID-endorsed consensus document for the diagnosis of spine infection (spondylodiscitis) in adults.

Authors:  Elena Lazzeri; Alessandro Bozzao; Maria Adriana Cataldo; Nicola Petrosillo; Luigi Manfrè; Andrej Trampuz; Alberto Signore; Mario Muto
Journal:  Eur J Nucl Med Mol Imaging       Date:  2019-08-09       Impact factor: 9.236

5.  Chronic brucellosis and persistence of Brucella melitensis DNA.

Authors:  Maria Jesús Castaño; Javier Solera
Journal:  J Clin Microbiol       Date:  2009-05-06       Impact factor: 5.948

6.  Comparative clinical study of different multiplex real time PCR strategies for the simultaneous differential diagnosis between extrapulmonary tuberculosis and focal complications of brucellosis.

Authors:  Rocio Sanjuan-Jimenez; Pilar Morata; Pilar Bermúdez; M José Bravo; Juan D Colmenero
Journal:  PLoS Negl Trop Dis       Date:  2013-12-12

Review 7.  Polymerase chain reaction-based assays for the diagnosis of human brucellosis.

Authors:  Ying Wang; Zhanli Wang; Yaxian Zhang; Liyun Bai; Yue Zhao; Chunfang Liu; An Ma; Hui Yu
Journal:  Ann Clin Microbiol Antimicrob       Date:  2014-08-01       Impact factor: 3.944
  7 in total

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