Literature DB >> 17965974

The construction and characteristics of a BAC library for Cucumis sativus L. 'B10'.

Wojciech Gutman1, Magdalena Pawełkowicz, Rafał Woycicki, Ewa Piszczek, Zbigniew Przybecki.   

Abstract

Cloning using bacterial artificial chromosomes (BACs) can yield high quality genomic libraries, which are used for the physical mapping, identification and isolation of genes, and for gene sequencing. A BAC genomic library was constructed from high molecular weight DNA (HMW DNA) obtained from nuclei of the cucumber (Cucumis sativus L. cv. Borszczagowski; B10 line). The DNA was digested with the HindIII restriction enzyme and ligated into the pCC1BAC vector. The library consists of 34,560 BAC clones with an average insert size of 135 kb, and 12.7x genome coverage. Screening the library for chloroplast and mitochondrial DNA content indicated an exceptionally low 0.26% contamination with chloroplast DNA and 0.3% with mitochondrial DNA.

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Year:  2007        PMID： 17965974      PMCID： PMC6275958          DOI： 10.2478/s11658-007-0038-6

Source DB:  PubMed          Journal:  Cell Mol Biol Lett        ISSN： 1425-8153            Impact factor:   5.787


  15 in total

1.  Construction of a yeast artificial chromosome library of tomato and identification of cloned segments linked to two disease resistance loci.

Authors:  G B Martin; M W Ganal; S D Tanksley
Journal:  Mol Gen Genet       Date:  1992-05

2.  Construction and characterization of a soybean bacterial artificial chromosome library and use of multiple complementary libraries for genome physical mapping.

Authors:  C-C Wu; P Nimmakayala; F A Santos; R Springman; C Scheuring; K Meksem; D A Lightfoot; H-B Zhang
Journal:  Theor Appl Genet       Date:  2004-05-26       Impact factor: 5.699

3.  Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector.

Authors:  H Shizuya; B Birren; U J Kim; V Mancino; T Slepak; Y Tachiiri; M Simon
Journal:  Proc Natl Acad Sci U S A       Date:  1992-09-15       Impact factor: 11.205

4.  Preparation of clone libraries in yeast artificial-chromosome vectors.

Authors:  D T Burke; M V Olson
Journal:  Methods Enzymol       Date:  1991       Impact factor: 1.600

5.  Construction of yeast artificial chromosome libraries with large inserts using fractionation by pulsed-field gel electrophoresis.

Authors:  R Anand; A Villasante; C Tyler-Smith
Journal:  Nucleic Acids Res       Date:  1989-05-11       Impact factor: 16.971

6.  Cross-species bacterial artificial chromosome (BAC) library screening via overgo-based hybridization and BAC-contig mapping of a yield enhancement quantitative trait locus (QTL) yld1.1 in the Malaysian wild rice Oryza rufipogon.

Authors:  Beng-Kah Song; Kalaivani Nadarajah; Michael N Romanov; Wickneswari Ratnam
Journal:  Cell Mol Biol Lett       Date:  2005       Impact factor: 5.787

7.  Pervasive migration of organellar DNA to the nucleus in plants.

Authors:  J L Blanchard; G W Schmidt
Journal:  J Mol Evol       Date:  1995-10       Impact factor: 2.395

8.  A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome.

Authors:  L Clarke; J Carbon
Journal:  Cell       Date:  1976-09       Impact factor: 41.582

9.  Construction of two BAC libraries from cucumber (Cucumis sativus L.) and identification of clones linked to yield component quantitative trait loci.

Authors:  Y-W Nam; J-R Lee; K-H Song; M-K Lee; M D Robbins; S-M Chung; J E Staub; H-B Zhang
Journal:  Theor Appl Genet       Date:  2005-04-30       Impact factor: 5.699

10.  Polymorphom of sexually different cucumber (Cucumis sativus L.) NIL lines.

Authors:  Zbigniew Przybecki; Magdalena Ewa Kowalczyk; Justyna Witkowicz; Marcin Filipecki; Ewa Siedlecka
Journal:  Cell Mol Biol Lett       Date:  2004       Impact factor: 5.787
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  4 in total

1.  Construction and characterization of two BAC libraries representing a deep-coverage of the genome of chicory (Cichorium intybus L., Asteraceae).

Authors:  Lucy Gonthier; Arnaud Bellec; Christelle Blassiau; Elisa Prat; Nicolas Helmstetter; Caroline Rambaud; Brigitte Huss; Theo Hendriks; Hélène Bergès; Marie-Christine Quillet
Journal:  BMC Res Notes       Date:  2010-08-11

2.  A high-quality cucumber genome assembly enhances computational comparative genomics.

Authors:  Paweł Osipowski; Magdalena Pawełkowicz; Michał Wojcieszek; Agnieszka Skarzyńska; Zbigniew Przybecki; Wojciech Pląder
Journal:  Mol Genet Genomics       Date:  2019-10-16       Impact factor: 3.291

3.  Adventures in the enormous: a 1.8 million clone BAC library for the 21.7 Gb genome of loblolly pine.

Authors:  Zenaida V Magbanua; Seval Ozkan; Benjamin D Bartlett; Philippe Chouvarine; Christopher A Saski; Aaron Liston; Richard C Cronn; C Dana Nelson; Daniel G Peterson
Journal:  PLoS One       Date:  2011-01-21       Impact factor: 3.240

4.  The genome sequence of the North-European cucumber (Cucumis sativus L.) unravels evolutionary adaptation mechanisms in plants.

Authors:  Rafał Wóycicki; Justyna Witkowicz; Piotr Gawroński; Joanna Dąbrowska; Alexandre Lomsadze; Magdalena Pawełkowicz; Ewa Siedlecka; Kohei Yagi; Wojciech Pląder; Anna Seroczyńska; Mieczysław Śmiech; Wojciech Gutman; Katarzyna Niemirowicz-Szczytt; Grzegorz Bartoszewski; Norikazu Tagashira; Yoshikazu Hoshi; Mark Borodovsky; Stanisław Karpiński; Stefan Malepszy; Zbigniew Przybecki
Journal:  PLoS One       Date:  2011-07-28       Impact factor: 3.240
  4 in total

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