Literature DB >> 2542900

Construction of yeast artificial chromosome libraries with large inserts using fractionation by pulsed-field gel electrophoresis.

R Anand1, A Villasante, C Tyler-Smith.   

Abstract

A method for constructing yeast artificial chromosome (YAC) libraries with large insert sizes is reported. High molecular weight human DNA was partially digested with EcoRI and cloned in the vector pYAC4. When unfractionated DNA was used, the mean YAC size was 120kb. Fractionation by pulsed-field gel electrophoresis using a 'waltzer' apparatus to remove small DNA fragments increased the mean YAC size to congruent to 220kb or congruent to 370kb depending on the fractionation conditions. Ligated DNA prepared by this method was stable at 4 degrees C and routinely yielded transformation efficiencies of greater than 700 colonies/micrograms. It should be possible to extend the method to produce even larger inserts and to use high molecular weight DNA from any source.

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Year:  1989        PMID： 2542900      PMCID： PMC317785          DOI： 10.1093/nar/17.9.3425

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  13 in total

1.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

2.  Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors.

Authors:  D T Burke; G F Carle; M V Olson
Journal:  Science       Date:  1987-05-15       Impact factor: 47.728

3.  Development of large DNA methods for plants: molecular cloning of large segments of Arabidopsis and carrot DNA into yeast.

Authors:  P Guzmán; J R Ecker
Journal:  Nucleic Acids Res       Date:  1988-12-09       Impact factor: 16.971

4.  A model for the separation of large DNA molecules by crossed field gel electrophoresis.

Authors:  E M Southern; R Anand; W R Brown; D S Fletcher
Journal:  Nucleic Acids Res       Date:  1987-08-11       Impact factor: 16.971

5.  Genome linking with yeast artificial chromosomes.

Authors:  A Coulson; R Waterston; J Kiff; J Sulston; Y Kohara
Journal:  Nature       Date:  1988-09-08       Impact factor: 49.962

6.  Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis.

Authors:  D C Schwartz; C R Cantor
Journal:  Cell       Date:  1984-05       Impact factor: 41.582

7.  Characterization of human chromosomal DNA sequences which replicate autonomously in Saccharomyces cerevisiae.

Authors:  J F Montiel; C J Norbury; M F Tuite; M J Dobson; J S Mills; A J Kingsman; S M Kingsman
Journal:  Nucleic Acids Res       Date:  1984-01-25       Impact factor: 16.971

8.  Restriction endonuclease analysis of ribosomal DNA from Saccharomyces cerevisiae.

Authors:  J H Cramer; F W Farrelly; R H Rownd
Journal:  Mol Gen Genet       Date:  1976-11-17

9.  Complete cloning of the Duchenne muscular dystrophy (DMD) cDNA and preliminary genomic organization of the DMD gene in normal and affected individuals.

Authors:  M Koenig; E P Hoffman; C J Bertelson; A P Monaco; C Feener; L M Kunkel
Journal:  Cell       Date:  1987-07-31       Impact factor: 41.582

10.  Synthesis of hybrid bacterial plasmids containing highly repeated satellite DNA.

Authors:  D Brutlag; K Fry; T Nelson; P Hung
Journal:  Cell       Date:  1977-03       Impact factor: 41.582
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  55 in total

1.  Construction of a yeast artificial chromosome library of tomato and identification of cloned segments linked to two disease resistance loci.

Authors:  G B Martin; M W Ganal; S D Tanksley
Journal:  Mol Gen Genet       Date:  1992-05

2.  Amplification of large artificial chromosomes.

Authors:  D R Smith; A P Smyth; D T Moir
Journal:  Proc Natl Acad Sci U S A       Date:  1990-11       Impact factor: 11.205

3.  Long range restriction analysis of the bovine casein genes.

Authors:  L Ferretti; P Leone; V Sgaramella
Journal:  Nucleic Acids Res       Date:  1990-12-11       Impact factor: 16.971

4.  Molecular analysis of the human MHC class I region using yeast artificial chromosome clones.

Authors:  G Chimini; J Boretto; D Marguet; F Lanau; G Lauquin; P Pontarotti
Journal:  Immunogenetics       Date:  1990       Impact factor: 2.846

5.  Yeast artificial chromosome libraries containing large inserts from mouse and human DNA.

Authors:  Z Larin; A P Monaco; H Lehrach
Journal:  Proc Natl Acad Sci U S A       Date:  1991-05-15       Impact factor: 11.205

6.  Construction and characterisation of a yeast artificial chromosome library containing three haploid maize genome equivalents.

Authors:  K J Edwards; H Thompson; D Edwards; A de Saizieu; C Sparks; J A Thompson; A J Greenland; M Eyers; W Schuch
Journal:  Plant Mol Biol       Date:  1992-05       Impact factor: 4.076

7.  Dodeca satellite: a conserved G+C-rich satellite from the centromeric heterochromatin of Drosophila melanogaster.

Authors:  J P Abad; M Carmena; S Baars; R D Saunders; D M Glover; P Ludeña; C Sentis; C Tyler-Smith; A Villasante
Journal:  Proc Natl Acad Sci U S A       Date:  1992-05-15       Impact factor: 11.205

Review 8.  Long-range walking techniques in positional cloning strategies.

Authors:  L Stubbs
Journal:  Mamm Genome       Date:  1992       Impact factor: 2.957

9.  Introduction of YACs into intact yeast cells by a procedure which shows low levels of recombinagenicity and co-transformation.

Authors:  S M Heale; L I Stateva; S G Oliver
Journal:  Nucleic Acids Res       Date:  1994-11-25       Impact factor: 16.971

10.  Construction and characterization of a bacterial artificial chromosome library of Sorghum bicolor.

Authors:  S S Woo; J Jiang; B S Gill; A H Paterson; R A Wing
Journal:  Nucleic Acids Res       Date:  1994-11-25       Impact factor: 16.971
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