Literature DB >> 17952628

A general method for hyperquenching protein crystals.

Matthew Warkentin1, Robert E Thorne.   

Abstract

During flash cooling of protein crystals in liquid cryogens, cooling rates are determined by sample size, choice of cooling liquid, and by the thickness of the cold gas layer that forms above the liquid. We describe an experimental protocol for ultra-rapid cooling of protein crystals. This protocol requires no complex apparatus, and yields ice-ring-free diffraction without the use of penetrating cryoprotectants.

Mesh：

Substances：

Year:  2007        PMID： 17952628      PMCID： PMC2779000          DOI： 10.1007/s10969-007-9029-0

Source DB:  PubMed          Journal:  J Struct Funct Genomics        ISSN： 1345-711X


  12 in total

1.  Cool data: quantity AND quality.

Authors:  E Garman
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  1999-10

2.  Heat transfer from protein crystals: implications for flash-cooling and X-ray beam heating.

Authors:  S Kriminski; M Kazmierczak; R E Thorne
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2003-03-25

Review 3.  Macromolecular cryocrystallography--methods for cooling and mounting protein crystals at cryogenic temperatures.

Authors:  J W Pflugrath
Journal:  Methods       Date:  2004-11       Impact factor: 3.608

4.  Biomolecular cryocrystallography: structural changes during flash-cooling.

Authors:  Bertil Halle
Journal:  Proc Natl Acad Sci U S A       Date:  2004-03-29       Impact factor: 11.205

5.  Hyperquenching for protein cryocrystallography.

Authors:  Matthew Warkentin; Viatcheslav Berejnov; Naji S Husseini; Robert E Thorne
Journal:  J Appl Crystallogr       Date:  2006-12-01       Impact factor: 3.304

Review 6.  Cryo-cooling in macromolecular crystallography: advantages, disadvantages and optimization.

Authors:  Douglas H Juers; Brian W Matthews
Journal:  Q Rev Biophys       Date:  2004-05       Impact factor: 5.318

7.  The molecular organization of cypovirus polyhedra.

Authors:  Fasséli Coulibaly; Elaine Chiu; Keiko Ikeda; Sascha Gutmann; Peter W Haebel; Clemens Schulze-Briese; Hajime Mori; Peter Metcalf
Journal:  Nature       Date:  2007-03-01       Impact factor: 49.962

8.  Cryocrystallography of biological macromolecules: a generally applicable method.

Authors:  H Hope
Journal:  Acta Crystallogr B       Date:  1988-02-01

Review 9.  Cryocrystallography.

Authors:  D W Rodgers
Journal:  Structure       Date:  1994-12-15       Impact factor: 5.006

10.  Structure of a B-DNA dodecamer at 16 K.

Authors:  H R Drew; S Samson; R E Dickerson
Journal:  Proc Natl Acad Sci U S A       Date:  1982-07       Impact factor: 11.205
View more
  9 in total

1.  First experiences with semi-autonomous robotic harvesting of protein crystals.

Authors:  Robert Viola; Jace Walsh; Alex Melka; Wesley Womack; Sean Murphy; Alan Riboldi-Tunnicliffe; Bernhard Rupp
Journal:  J Struct Funct Genomics       Date:  2011-03-23

2.  Approaches to automated protein crystal harvesting.

Authors:  Marc C Deller; Bernhard Rupp
Journal:  Acta Crystallogr F Struct Biol Commun       Date:  2014-01-28       Impact factor: 1.056

3.  D3R Grand Challenge 2: blind prediction of protein-ligand poses, affinity rankings, and relative binding free energies.

Authors:  Zied Gaieb; Shuai Liu; Symon Gathiaka; Michael Chiu; Huanwang Yang; Chenghua Shao; Victoria A Feher; W Patrick Walters; Bernd Kuhn; Markus G Rudolph; Stephen K Burley; Michael K Gilson; Rommie E Amaro
Journal:  J Comput Aided Mol Des       Date:  2017-12-04       Impact factor: 3.686

4.  Structural origins of nitroxide side chain dynamics on membrane protein α-helical sites.

Authors:  Brett M Kroncke; Peter S Horanyi; Linda Columbus
Journal:  Biochemistry       Date:  2010-11-08       Impact factor: 3.162

5.  The Thermus thermophilus DEAD box helicase Hera contains a modified RNA recognition motif domain loosely connected to the helicase core.

Authors:  Markus G Rudolph; Dagmar Klostermeier
Journal:  RNA       Date:  2009-08-26       Impact factor: 4.942

6.  Recognition of two distinct elements in the RNA substrate by the RNA-binding domain of the T. thermophilus DEAD box helicase Hera.

Authors:  Lenz Steimer; Jan Philip Wurm; Martin H Linden; Markus G Rudolph; Jens Wöhnert; Dagmar Klostermeier
Journal:  Nucleic Acids Res       Date:  2013-04-25       Impact factor: 16.971

7.  Crystal structures of Thermotoga maritima reverse gyrase: inferences for the mechanism of positive DNA supercoiling.

Authors:  Markus G Rudolph; Yoandris del Toro Duany; Stefan P Jungblut; Agneyo Ganguly; Dagmar Klostermeier
Journal:  Nucleic Acids Res       Date:  2012-12-02       Impact factor: 16.971

8.  Mapping the conformational space accessible to catechol-O-methyltransferase.

Authors:  Andreas Ehler; Jörg Benz; Daniel Schlatter; Markus G Rudolph
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2014-07-25

9.  Effectiveness of different types of mask in aerosol dispersion in SARS-CoV-2 infection.

Authors:  Gokhan Tanisali; Ahmet Sozak; Abdul Samet Bulut; Tolga Ziya Sander; Ozlem Dogan; Çağdaş Dağ; Mehmet Gönen; Fusun Can; Hasan DeMirci; Onder Ergonul
Journal:  Int J Infect Dis       Date:  2021-06-17       Impact factor: 3.623
  9 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.