Literature DB >> 17933922

Enumeration of viable Listeria monocytogenes cells by real-time PCR with propidium monoazide and ethidium monoazide in the presence of dead cells.

Y Pan1, F Breidt.   

Abstract

Propidium monoazide (PMA) and ethidium monoazide were used for enumeration of viable Listeria monocytogenes cells in the presence of dead cells. PMA had no antimicrobial effect on L. monocytogenes. Viable cell counts were linearly related to real-time PCR threshold cycle values for PMA-treated cells from planktonic and biofilm sources over a 4-log range.

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Year:  2007        PMID： 17933922      PMCID： PMC2168130          DOI： 10.1128/AEM.01198-07

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  16 in total

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3.  Resistance of Listeria monocytogenes biofilms to sanitizing agents in a simulated food processing environment.

Authors:  Y Pan; F Breidt; S Kathariou
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5.  Ethidium monoazide for DNA-based differentiation of viable and dead bacteria by 5'-nuclease PCR.

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Journal:  Biotechniques       Date:  2003-04       Impact factor: 1.993

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Authors:  H K Nogva; K Rudi; K Naterstad; A Holck; D Lillehaug
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8.  Differentiation of the major Listeria monocytogenes serovars by multiplex PCR.

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Journal:  J Food Prot       Date:  2002-08       Impact factor: 2.077
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Authors:  Sandhya Parshionikar; Ian Laseke; G Shay Fout
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3.  Rapid detection of viable salmonellae in produce by coupling propidium monoazide with loop-mediated isothermal amplification.

Authors:  Siyi Chen; Fei Wang; John C Beaulieu; Rebecca E Stein; Beilei Ge
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Review 4.  Ecology and exploration of the rare biosphere.

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6.  Application of EMA-qPCR as a complementary tool for the detection and monitoring of Legionella in different water systems.

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Journal:  World J Microbiol Biotechnol       Date:  2012-01-05       Impact factor: 3.312

7.  Innovative Use of Palladium Compounds To Selectively Detect Live Enterobacteriaceae in Milk by PCR.

Authors:  Takashi Soejima; Kei-Ji Iwatsuki
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8.  Stress- and growth rate-related differences between plate count and real-time PCR data during growth of Listeria monocytogenes.

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9.  Discrimination of viable and dead fecal Bacteroidales bacteria by quantitative PCR with propidium monoazide.

Authors:  Sungwoo Bae; Stefan Wuertz
Journal:  Appl Environ Microbiol       Date:  2009-03-06       Impact factor: 4.792

10.  Detection and Quantification of Viable and Nonviable Trypanosoma cruzi Parasites by a Propidium Monoazide Real-Time Polymerase Chain Reaction Assay.

Authors:  Beatriz Cancino-Faure; Roser Fisa; M Magdalena Alcover; Teresa Jimenez-Marco; Cristina Riera
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