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Literature DB >> 17932509

Proteomic and functional analysis of Argonaute-containing mRNA-protein complexes in human cells.

Julia Höck¹, Lasse Weinmann, Christine Ender, Sabine Rüdel, Elisabeth Kremmer, Monika Raabe, Henning Urlaub, Gunter Meister.

Abstract

Members of the Argonaute (Ago) protein family associate with small RNAs and have important roles in RNA silencing. Here, we analysed Ago1- and Ago2-containing protein complexes in human cells. Separation of Ago-associated messenger ribonucleoproteins (mRNPs) showed that Ago1 and Ago2 reside in three complexes with distinct Dicer and RNA-induced silencing complex activities. A comprehensive proteomic analysis of Ago-containing mRNPs identified a large number of proteins involved in RNA metabolism. By using co-immunoprecipitation experiments followed by RNase treatment, we biochemically mapped interactions within Ago mRNPs. Using reporter assays and knockdown experiments, we showed that the putative RNA-binding protein RBM4 is required for microRNA-guided gene regulation.

Entities: Gene Species

Mesh：

Substances：

Year: 2007 PMID： 17932509 PMCID： PMC2247381 DOI： 10.1038/sj.embor.7401088

Source DB: PubMed Journal: EMBO Rep ISSN： 1469-221X Impact factor: 8.807

28 in total

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7. MicroRNA-dependent localization of targeted mRNAs to mammalian P-bodies.

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8. RAS is regulated by the let-7 microRNA family.

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9. Human Argonaute2 mediates RNA cleavage targeted by miRNAs and siRNAs.

Authors: Gunter Meister; Markus Landthaler; Agnieszka Patkaniowska; Yair Dorsett; Grace Teng; Thomas Tuschl
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10. Identification of human microRNA targets from isolated argonaute protein complexes.

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189 in total

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5. Subnuclear targeting of the RNA-binding motif protein RBM6 to splicing speckles and nascent transcripts.

Authors: Emma Heath; Fred Sablitzky; Garry T Morgan
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Review 6. MicroRNAs in embryonic stem cell function and fate.

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7. PRMT1 methylates the single Argonaute of Toxoplasma gondii and is important for the recruitment of Tudor nuclease for target RNA cleavage by antisense guide RNA.

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Journal: Cell Microbiol Date: 2012-02-28 Impact factor: 3.715