Literature DB >> 17634606

Production of protein for nuclear magnetic resonance study using the wheat germ cell-free system.

Toshiyuki Kohno1, Yaeta Endo.   

Abstract

Nuclear magnetic resonance (NMR) methods have been developed to determine the three-dimensional structures of proteins, to estimate protein folding, and to discover high-affinity ligands for proteins. However, one of the difficulties encountered in the application of such NMR methods to proteins is that we should obtain milligram quantities of 15N and/or 13C-labeled pure proteins of interest. Here, we describe the method to produce proteins for NMR experiments using the improved wheat germ cell-free system, which exhibits several attractive features for high-throughput NMR study of proteins.

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Year:  2007        PMID： 17634606     DOI： 10.1007/978-1-59745-388-2_13

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  10 in total

1.  NMR-based characterization of a refolding intermediate of beta2-microglobulin labeled using a wheat germ cell-free system.

Authors:  Atsushi Kameda; Eugene-Hayato Morita; Kazumasa Sakurai; Hironobu Naiki; Yuji Goto
Journal:  Protein Sci       Date:  2009-08       Impact factor: 6.725

2.  Practical cell-free protein synthesis system using purified wheat embryos.

Authors:  Kazuyuki Takai; Tatsuya Sawasaki; Yaeta Endo
Journal:  Nat Protoc       Date:  2010-01-21       Impact factor: 13.491

Review 3.  Advances in protein NMR provided by the NIGMS Protein Structure Initiative: impact on drug discovery.

Authors:  Gaetano T Montelione; Thomas Szyperski
Journal:  Curr Opin Drug Discov Devel       Date:  2010-05

4.  Involvement of the N-terminal B-box domain of Arabidopsis BBX32 protein in interaction with soybean BBX62 protein.

Authors:  Qungang Qi; Ann Gibson; Xiaoran Fu; Meiying Zheng; Rosemarie Kuehn; Yongcheng Wang; Yanfei Wang; Santiago Navarro; James A Morrell; Dongming Jiang; Grant Simmons; Erin Bell; Natalia B Ivleva; Amanda L McClerren; Paul Loida; Thomas G Ruff; Marie E Petracek; Sasha B Preuss
Journal:  J Biol Chem       Date:  2012-07-16       Impact factor: 5.157

5.  Active-site structure of the thermophilic Foc-subunit ring in membranes elucidated by solid-state NMR.

Authors:  Su-Jin Kang; Yasuto Todokoro; Ikuko Yumen; Bo Shen; Iku Iwasaki; Toshiharu Suzuki; Atsushi Miyagi; Masasuke Yoshida; Toshimichi Fujiwara; Hideo Akutsu
Journal:  Biophys J       Date:  2014-01-21       Impact factor: 4.033

6.  Engineering of a wheat germ expression system to provide compatibility with a high throughput pET-based cloning platform.

Authors:  Li Zhao; Kate Q Zhao; Robin Hurst; Michael R Slater; Thomas B Acton; G V T Swapna; Ritu Shastry; Gregory J Kornhaber; Gaetano T Montelione
Journal:  J Struct Funct Genomics       Date:  2010-06-24

7.  Functional protein expression from a DNA based wheat germ cell-free system.

Authors:  Kate Qin Zhao; Robin Hurst; Michael R Slater; Robert F Bulleit
Journal:  J Struct Funct Genomics       Date:  2007-11-23

8.  Development of a cell-free protein synthesis system for practical use.

Authors:  Yaeta Endo
Journal:  Proc Jpn Acad Ser B Phys Biol Sci       Date:  2021       Impact factor: 3.493

Review 9.  Easy Synthesis of Complex Biomolecular Assemblies: Wheat Germ Cell-Free Protein Expression in Structural Biology.

Authors:  Marie-Laure Fogeron; Lauriane Lecoq; Laura Cole; Matthias Harbers; Anja Böckmann
Journal:  Front Mol Biosci       Date:  2021-03-25

Review 10.  Solid-State NMR for Studying the Structure and Dynamics of Viral Assemblies.

Authors:  Lauriane Lecoq; Marie-Laure Fogeron; Beat H Meier; Michael Nassal; Anja Böckmann
Journal:  Viruses       Date:  2020-09-24       Impact factor: 5.048
  10 in total

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