Literature DB >> 17609326

Development and application of a novel peptide nucleic acid probe for the specific detection of Helicobacter pylori in gastric biopsy specimens.

N Guimarães1, N F Azevedo, C Figueiredo, C W Keevil, M J Vieira.   

Abstract

In this work, a fluorescence in situ hybridization (FISH) method for the rapid detection of Helicobacter pylori using a novel peptide nucleic acid (PNA) probe is reported. Laboratory testing with several different bacterial species, including other Helicobacter spp., has shown that this probe is highly specific for H. pylori strains. In addition, the PNA FISH method has been successfully adapted for detection of the pathogen in paraffin-embedded gastric biopsy specimens.

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Year:  2007        PMID： 17609326      PMCID： PMC2045269          DOI： 10.1128/JCM.00858-07

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  32 in total

Review 1.  PNA for rapid microbiology.

Authors:  Henrik Stender; Mark Fiandaca; Jens J Hyldig-Nielsen; James Coull
Journal:  J Microbiol Methods       Date:  2002-01       Impact factor: 2.363

Review 2.  Fluorescence in situ hybridization (FISH) for direct visualization of microorganisms.

Authors:  A Moter; U B Göbel
Journal:  J Microbiol Methods       Date:  2000-07       Impact factor: 2.363

3.  PRIMROSE: a computer program for generating and estimating the phylogenetic range of 16S rRNA oligonucleotide probes and primers in conjunction with the RDP-II database.

Authors:  Kevin E Ashelford; Andrew J Weightman; John C Fry
Journal:  Nucleic Acids Res       Date:  2002-08-01       Impact factor: 16.971

Review 4.  Peptide nucleic acid: a versatile tool in genetic diagnostics and molecular biology.

Authors:  P E Nielsen
Journal:  Curr Opin Biotechnol       Date:  2001-02       Impact factor: 9.740

5.  Fluorescence in situ hybridization using peptide nucleic acid probes for rapid detection of Mycobacterium avium subsp. avium and Mycobacterium avium subsp. paratuberculosis in potable-water biofilms.

Authors:  Markku J Lehtola; Eila Torvinen; Ilkka T Miettinen; C William Keevil
Journal:  Appl Environ Microbiol       Date:  2006-01       Impact factor: 4.792

6.  Identification of indicator microorganisms using a standardized PNA FISH method.

Authors:  H Perry-O'Keefe; S Rigby; K Oliveira; D Sørensen; H Stender; J Coull; J J Hyldig-Nielsen
Journal:  J Microbiol Methods       Date:  2001-12       Impact factor: 2.363

7.  Comparison of fluorescent in situ hybridization and conventional culturing for detection of Helicobacter pylori in gastric biopsy specimens.

Authors:  H Rüssmann; V A Kempf; S Koletzko; J Heesemann; I B Autenrieth
Journal:  J Clin Microbiol       Date:  2001-01       Impact factor: 5.948

8.  The stringent response is required for Helicobacter pylori survival of stationary phase, exposure to acid, and aerobic shock.

Authors:  Kyle Mouery; Bethany A Rader; Erin C Gaynor; Karen Guillemin
Journal:  J Bacteriol       Date:  2006-08       Impact factor: 3.490

9.  Application of fluorescent in situ hybridization (FISH) for the detection of Helicobacter pylori.

Authors:  Ali Reza Samarbaf-Zadeh; Saeed Tajbakhsh; Seid Mojtaba Moosavian; Majid Sadeghi-Zadeh; Mehrdad Azmi; Jalal Hashemi; Abdolrahim Masjedi-Zadeh
Journal:  Med Sci Monit       Date:  2006-09-25

10.  New screening software shows that most recent large 16S rRNA gene clone libraries contain chimeras.

Authors:  Kevin E Ashelford; Nadia A Chuzhanova; John C Fry; Antonia J Jones; Andrew J Weightman
Journal:  Appl Environ Microbiol       Date:  2006-09       Impact factor: 4.792
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  20 in total

1.  Fluorescence in situ hybridization method using a peptide nucleic acid probe for identification of Salmonella spp. in a broad spectrum of samples.

Authors:  C Almeida; N F Azevedo; R M Fernandes; C W Keevil; M J Vieira
Journal:  Appl Environ Microbiol       Date:  2010-05-07       Impact factor: 4.792

2.  Development and application of a novel peptide nucleic acid probe for the specific detection of Cronobacter genomospecies (Enterobacter sakazakii) in powdered infant formula.

Authors:  C Almeida; N F Azevedo; C Iversen; S Fanning; C W Keevil; M J Vieira
Journal:  Appl Environ Microbiol       Date:  2009-03-06       Impact factor: 4.792

3.  Application of Nucleic Acid Mimics in Fluorescence In Situ Hybridization.

Authors:  Ricardo Oliveira; Andreia S Azevedo; Luzia Mendes
Journal:  Methods Mol Biol       Date:  2021

4.  Bioinformatic Tools and Guidelines for the Design of Fluorescence In Situ Hybridization Probes.

Authors:  Helena Teixeira; Ana L Sousa; Andreia S Azevedo
Journal:  Methods Mol Biol       Date:  2021

5.  An Introduction to Fluorescence in situ Hybridization in Microorganisms.

Authors:  Carina Almeida; Nuno F Azevedo
Journal:  Methods Mol Biol       Date:  2021

6.  Effect of chlorine on incorporation of Helicobacter pylori into drinking water biofilms.

Authors:  M S Gião; N F Azevedo; S A Wilks; M J Vieira; C W Keevil
Journal:  Appl Environ Microbiol       Date:  2009-12-04       Impact factor: 4.792

7.  Persistence of Helicobacter pylori in heterotrophic drinking-water biofilms.

Authors:  M S Gião; N F Azevedo; S A Wilks; M J Vieira; C W Keevil
Journal:  Appl Environ Microbiol       Date:  2008-08-01       Impact factor: 4.792

8.  Validation of a fluorescence in situ hybridization method using peptide nucleic acid probes for detection of Helicobacter pylori clarithromycin resistance in gastric biopsy specimens.

Authors:  Laura Cerqueira; Ricardo M Fernandes; Rui M Ferreira; Mónica Oleastro; Fátima Carneiro; Catarina Brandão; Pedro Pimentel-Nunes; Mário Dinis-Ribeiro; Céu Figueiredo; Charles W Keevil; Maria J Vieira; Nuno F Azevedo
Journal:  J Clin Microbiol       Date:  2013-04-17       Impact factor: 5.948

9.  Evaluation of a new test, genotype HelicoDR, for molecular detection of antibiotic resistance in Helicobacter pylori.

Authors:  Emmanuelle Cambau; Vera Allerheiligen; Céline Coulon; Céline Corbel; Christine Lascols; Lionel Deforges; Claude-James Soussy; Jean-Charles Delchier; Francis Megraud
Journal:  J Clin Microbiol       Date:  2009-09-16       Impact factor: 5.948

10.  DNA mimics for the rapid identification of microorganisms by fluorescence in situ hybridization (FISH).

Authors:  Laura Cerqueira; Nuno F Azevedo; Carina Almeida; Tatiana Jardim; Charles William Keevil; Maria J Vieira
Journal:  Int J Mol Sci       Date:  2008-10-20       Impact factor: 5.923
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