Literature DB >> 19270117

Development and application of a novel peptide nucleic acid probe for the specific detection of Cronobacter genomospecies (Enterobacter sakazakii) in powdered infant formula.

C Almeida1, N F Azevedo, C Iversen, S Fanning, C W Keevil, M J Vieira.   

Abstract

Here, we report a fluorescence in situ hybridization (FISH) method for rapid detection of Cronobacter strains in powdered infant formula (PIF) using a novel peptide nucleic acid (PNA) probe. Laboratory tests with several Enterobacteriaceae species showed that the specificity and sensitivity of the method were 100%. FISH using PNA could detect as few as 1 CFU per 10 g of Cronobacter in PIF after an 8-h enrichment step, even in a mixed population containing bacterial contaminants.

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Year:  2009        PMID： 19270117      PMCID： PMC2681712          DOI： 10.1128/AEM.02470-08

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  45 in total

1.  Direct identification of major blood culture pathogens, including Pseudomonas aeruginosa and Escherichia coli, by a panel of fluorescence in situ hybridization assays using peptide nucleic acid probes.

Authors:  M Søgaard; H Stender; H C Schønheyder
Journal:  J Clin Microbiol       Date:  2005-04       Impact factor: 5.948

2.  Advantages of peptide nucleic acid oligonucleotides for sensitive site directed 16S rRNA fluorescence in situ hybridization (FISH) detection of Campylobacter jejuni, Campylobacter coli and Campylobacter lari.

Authors:  Markku J Lehtola; Christopher J Loades; C William Keevil
Journal:  J Microbiol Methods       Date:  2005-03-19       Impact factor: 2.363

3.  Multicenter evaluation of a Candida albicans peptide nucleic acid fluorescent in situ hybridization probe for characterization of yeast isolates from blood cultures.

Authors:  D A Wilson; M J Joyce; L S Hall; L B Reller; G D Roberts; G S Hall; B D Alexander; G W Procop
Journal:  J Clin Microbiol       Date:  2005-06       Impact factor: 5.948

4.  Use of PNA oligonucleotides for the in situ detection of Escherichia coli in water.

Authors:  A M Prescott; C R Fricker
Journal:  Mol Cell Probes       Date:  1999-08       Impact factor: 2.365

5.  Rapid, specific detection of Enterobacter sakazakii in infant formula using a real-time PCR assay.

Authors:  K H Seo; R E Brackett
Journal:  J Food Prot       Date:  2005-01       Impact factor: 2.077

Review 6.  Enterobacter sakazakii: a coliform of increased concern to infant health.

Authors:  Joshua B Gurtler; Jeffrey L Kornacki; Larry R Beuchat
Journal:  Int J Food Microbiol       Date:  2005-09-25       Impact factor: 5.277

Review 7.  Bacillus cereus in infant foods and dried milk products.

Authors:  H Becker; G Schaller; W von Wiese; G Terplan
Journal:  Int J Food Microbiol       Date:  1994-09       Impact factor: 5.277

8.  Enterobacter sakazakii infections in neonates associated with intrinsic contamination of a powdered infant formula.

Authors:  B P Simmons; M S Gelfand; M Haas; L Metts; J Ferguson
Journal:  Infect Control Hosp Epidemiol       Date:  1989-09       Impact factor: 3.254

9.  The Ribosomal Database Project (RDP-II): sequences and tools for high-throughput rRNA analysis.

Authors:  J R Cole; B Chai; R J Farris; Q Wang; S A Kulam; D M McGarrell; G M Garrity; J M Tiedje
Journal:  Nucleic Acids Res       Date:  2005-01-01       Impact factor: 16.971

10.  16S rRNA gene based analysis of Enterobacter sakazakii strains from different sources and development of a PCR assay for identification.

Authors:  Angelika Lehner; Taurai Tasara; Roger Stephan
Journal:  BMC Microbiol       Date:  2004-11-25       Impact factor: 3.605
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  14 in total

1.  Fluorescence in situ hybridization method using a peptide nucleic acid probe for identification of Salmonella spp. in a broad spectrum of samples.

Authors:  C Almeida; N F Azevedo; R M Fernandes; C W Keevil; M J Vieira
Journal:  Appl Environ Microbiol       Date:  2010-05-07       Impact factor: 4.792

2.  FISH in Food Samples.

Authors:  Rui Rocha; Carina Almeida; Nuno F Azevedo
Journal:  Methods Mol Biol       Date:  2021

3.  Rapid detection of urinary tract infections caused by Proteus spp. using PNA-FISH.

Authors:  C Almeida; N F Azevedo; J C Bento; N Cerca; H Ramos; M J Vieira; C W Keevil
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2013-01-04       Impact factor: 3.267

4.  Detection of Escherichia coli O157 by peptide nucleic acid fluorescence in situ hybridization (PNA-FISH) and comparison to a standard culture method.

Authors:  C Almeida; J M Sousa; R Rocha; L Cerqueira; S Fanning; N F Azevedo; M J Vieira
Journal:  Appl Environ Microbiol       Date:  2013-08-09       Impact factor: 4.792

5.  Discriminating multi-species populations in biofilms with peptide nucleic acid fluorescence in situ hybridization (PNA FISH).

Authors:  Carina Almeida; Nuno F Azevedo; Sílvio Santos; Charles W Keevil; Maria J Vieira
Journal:  PLoS One       Date:  2011-03-29       Impact factor: 3.240

6.  PNA-FISH as a new diagnostic method for the determination of clarithromycin resistance of Helicobacter pylori.

Authors:  Laura Cerqueira; Ricardo M Fernandes; Rui M Ferreira; Fátima Carneiro; Mário Dinis-Ribeiro; Céu Figueiredo; Charles W Keevil; Nuno F Azevedo; Maria J Vieira
Journal:  BMC Microbiol       Date:  2011-05-14       Impact factor: 3.605

7.  Diagnosis of bacterial vaginosis by a new multiplex peptide nucleic acid fluorescence in situ hybridization method.

Authors:  António Machado; Joana Castro; Tatiana Cereija; Carina Almeida; Nuno Cerca
Journal:  PeerJ       Date:  2015-02-17       Impact factor: 2.984

Review 8.  Review article: the global emergence of Helicobacter pylori antibiotic resistance.

Authors:  I Thung; H Aramin; V Vavinskaya; S Gupta; J Y Park; S E Crowe; M A Valasek
Journal:  Aliment Pharmacol Ther       Date:  2015-12-23       Impact factor: 8.171

9.  Fluorescence in situ Hybridization method using Peptide Nucleic Acid probes for rapid detection of Lactobacillus and Gardnerella spp.

Authors:  António Machado; Carina Almeida; Débora Salgueiro; Ana Henriques; Mario Vaneechoutte; Freddy Haesebrouck; Maria João Vieira; Ligia Rodrigues; Nuno Filipe Azevedo; Nuno Cerca
Journal:  BMC Microbiol       Date:  2013-04-12       Impact factor: 3.605

Review 10.  Pathogens protection against the action of disinfectants in multispecies biofilms.

Authors:  Pilar Sanchez-Vizuete; Belen Orgaz; Stéphane Aymerich; Dominique Le Coq; Romain Briandet
Journal:  Front Microbiol       Date:  2015-07-14       Impact factor: 5.640
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