BACKGROUND AND AIM: Peritoneal adhesions are caused by intra-abdominal surgery and can lead to relevant complications. Adhesions are supposed to consist of avascular scar tissue. The aim of the present study was to analyze whether mature postsurgical adhesions even after years still reveal a dynamic remodeling process. MATERIALS AND METHODS: In a prospective analysis, we investigated tissue specimen of peritoneal adhesions in 40 patients after abdominal surgery. Expression of five parameters representing wound healing and remodeling were examined (MMP-2, Ki-67, apoptosis, collagen/protein ratio, and collagen type I/III ratio). RESULTS: Gender, age, and the number of previous operations had no impact on the parameters measured. Adhesion specimens were cell rich, containing mononuclear round cells, fibroblasts, adipose cells, and vascular endothelial cells. There was a positive expression of MMP-2 and apoptosis, whereas Ki-67 was marginal irrespective of adhesion maturity or quality. Adhesions classified as dense showed a significant increase in total collagen (118.2 +/- 4.9 microg/mg) and collagen type I/III ratios (3.9 +/- 0.2), whereas there were no significant differences regarding the adhesion maturity. CONCLUSION: The distinct composition of cellular components as well as of extracellular matrix proteins may reflect an interactive cross-talk between adhesion- and stroma-derived cells even in mature adhesions. Our findings support the hypothesis that the disabilities of appropriate repair of the peritoneal surface leading to persistent adhesions are a consequence of a permanent process of disturbed remodeling.
BACKGROUND AND AIM: Peritoneal adhesions are caused by intra-abdominal surgery and can lead to relevant complications. Adhesions are supposed to consist of avascular scar tissue. The aim of the present study was to analyze whether mature postsurgical adhesions even after years still reveal a dynamic remodeling process. MATERIALS AND METHODS: In a prospective analysis, we investigated tissue specimen of peritoneal adhesions in 40 patients after abdominal surgery. Expression of five parameters representing wound healing and remodeling were examined (MMP-2, Ki-67, apoptosis, collagen/protein ratio, and collagen type I/III ratio). RESULTS: Gender, age, and the number of previous operations had no impact on the parameters measured. Adhesion specimens were cell rich, containing mononuclear round cells, fibroblasts, adipose cells, and vascular endothelial cells. There was a positive expression of MMP-2 and apoptosis, whereas Ki-67 was marginal irrespective of adhesion maturity or quality. Adhesions classified as dense showed a significant increase in total collagen (118.2 +/- 4.9 microg/mg) and collagen type I/III ratios (3.9 +/- 0.2), whereas there were no significant differences regarding the adhesion maturity. CONCLUSION: The distinct composition of cellular components as well as of extracellular matrix proteins may reflect an interactive cross-talk between adhesion- and stroma-derived cells even in mature adhesions. Our findings support the hypothesis that the disabilities of appropriate repair of the peritoneal surface leading to persistent adhesions are a consequence of a permanent process of disturbed remodeling.
Authors: Marcel Binnebösel; Christian Daniel Klink; Julia Serno; Petra Lynen Jansen; Klaus Thilo von Trotha; Ulf Peter Neumann; Karsten Junge Journal: Langenbecks Arch Surg Date: 2011-02-01 Impact factor: 3.445
Authors: M Ditzel; E B Deerenberg; N Grotenhuis; J J Harlaar; K Monkhorst; Y M Bastiaansen-Jenniskens; J Jeekel; J F Lange Journal: Surg Endosc Date: 2013-04-03 Impact factor: 4.584
Authors: Christian D Klink; Patrick Schickhaus; Marcel Binnebösel; Stefan Jockenhoevel; Rafael Rosch; Rene Tolba; Ulf P Neumann; Uwe Klinge Journal: BMC Surg Date: 2013-09-10 Impact factor: 2.102