PURPOSE: Multimodal instrumentation is a new technical approach allowing simultaneous and complementary in vivo recordings of complementary biological parameters. To elucidate further the physiopathological mechanisms in intact small animal models, especially for brain studies, a challenging issue is the actual coupling of magnetic resonance imaging (MRI) techniques with positron emission tomography (PET): it has been shown that running the technology for radioactive imaging in a magnet alters the spatiotemporal performance of both modalities. Thus, we propose an alternative coupling of techniques that uses the beta-MicroProbe instead of PET for local measurements of radioactivity coupled with MRI. METHODS: We simultaneously recorded local radioactivity due to [(18)F]MPPF (a 5-HT(1A) receptor PET radiotracer) binding in the hippocampus with the beta-MicroProbe and carried out anatomical MRI in the same anaesthetised rat. RESULTS: The comparison of [(18)F]MPPF kinetics obtained from animals in a magnet with kinetics from a control group outside the magnet allowed us to determine the stability of tracer biokinetic measurements over time in the magnet. We were thus able to show that the beta-MicroProbe reliably measures radioactivity in rat brains under an intense magnetic field of 7 Tesla. CONCLUSION: The biological validation of a beta-MicroProbe/MRI dual system reported here opens up a wide range of future multimodal approaches for functional and pharmacological measurements by the probe combined with various magnetic resonance technologies, including anatomical MRI, functional MRI and MR spectroscopy.
PURPOSE: Multimodal instrumentation is a new technical approach allowing simultaneous and complementary in vivo recordings of complementary biological parameters. To elucidate further the physiopathological mechanisms in intact small animal models, especially for brain studies, a challenging issue is the actual coupling of magnetic resonance imaging (MRI) techniques with positron emission tomography (PET): it has been shown that running the technology for radioactive imaging in a magnet alters the spatiotemporal performance of both modalities. Thus, we propose an alternative coupling of techniques that uses the beta-MicroProbe instead of PET for local measurements of radioactivity coupled with MRI. METHODS: We simultaneously recorded local radioactivity due to [(18)F]MPPF (a 5-HT(1A) receptor PET radiotracer) binding in the hippocampus with the beta-MicroProbe and carried out anatomical MRI in the same anaesthetised rat. RESULTS: The comparison of [(18)F]MPPF kinetics obtained from animals in a magnet with kinetics from a control group outside the magnet allowed us to determine the stability of tracer biokinetic measurements over time in the magnet. We were thus able to show that the beta-MicroProbe reliably measures radioactivity in rat brains under an intense magnetic field of 7 Tesla. CONCLUSION: The biological validation of a beta-MicroProbe/MRI dual system reported here opens up a wide range of future multimodal approaches for functional and pharmacological measurements by the probe combined with various magnetic resonance technologies, including anatomical MRI, functional MRI and MR spectroscopy.
Authors: A Plenevaux; D Weissmann; J Aerts; C Lemaire; C Brihaye; C Degueldre; D Le Bars; D Comar; J Pujol; A Luxen Journal: J Neurochem Date: 2000-08 Impact factor: 5.372
Authors: Bernd J Pichler; Martin S Judenhofer; Ciprian Catana; Jeffrey H Walton; Manfred Kneilling; Robert E Nutt; Stefan B Siegel; Claus D Claussen; Simon R Cherry Journal: J Nucl Med Date: 2006-04 Impact factor: 10.057
Authors: Ciprian Catana; Yibao Wu; Martin S Judenhofer; Jinyi Qi; Bernd J Pichler; Simon R Cherry Journal: J Nucl Med Date: 2006-12 Impact factor: 10.057
Authors: L Balasse; J Maerk; F Pain; A Genoux; S Fieux; C Morel; P Gisquet-Verrier; L Zimmer; P Lanièce Journal: Mol Imaging Biol Date: 2015-04 Impact factor: 3.488