| Literature DB >> 15917749 |
Fawzi Boumezbeur1, Laurent Besret, Julien Valette, Marie-Claude Gregoire, Thierry Delzescaux, Renaud Maroy, Françoise Vaufrey, Philippe Gervais, Philippe Hantraye, Gilles Bloch, Vincent Lebon.
Abstract
The glycolytic flux (cerebral metabolic rate of glucose CMRglc) and the TCA cycle flux (VTCA) were measured in the same monkeys by 18F-fluorodeoxyglucose (18F-FDG) positron emission tomography (PET) and 13C NMR spectroscopy, respectively. Registration of nuclear magnetic resonance (NMR) and PET data were used for comparison of CMRglc and VTCA in the exact same area of the brain. Both fluxes were in good agreement with literature values (CMRglc=0.23+/-0.03 micromol/g min, VTCA=0.53+/-0.13 micromol/g min). The resulting [CMRglc/VTCA] ratio was 0.46+/-0.12 (n=5, mean+/-s.d.), not significantly different from the 0.5 expected when glucose is the sole fuel that is completely oxidized. Our results provide a cross-validation of both techniques. Comparison of CMRglc with VTCA is in agreement with a metabolic coupling between the TCA cycle and glycolysis under normal physiologic conditions.Entities:
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Year: 2005 PMID： 15917749 DOI： 10.1038/sj.jcbfm.9600145
Source DB: PubMed Journal: J Cereb Blood Flow Metab ISSN： 0271-678X Impact factor: 6.200