PURPOSE: The aim of this work was to demonstrate the pharmacokinetic potential of a wireless pixelated β(+)-sensitive probe (PIXSIC). PROCEDURES: The binding of 2'-methoxyphenyl-(N-2'-pyridinyl)-p-[(18)F]fluoro-benzamidoethylpiperazine ([(18)F]MPPF), a 5-HT1A serotonin receptor radiopharmaceutical, was measured in anesthetized rats and compared to microPET data. The effects of a 5-HT1A antagonist injection on in vivo [(18)F]MPPF binding were monitored by PIXSIC. RESULTS: PIXSIC allowed differentiating the radioactive kinetics according to the location of its pixels in the hippocampus, cortex, corpus callosum, and cerebellum. The device accurately detected the changes in [(18)F]MPPF binding, after 5-HT1A antagonist blockade. The time-activity curves were reproducible and consistent with kinetics obtained simultaneously with a microPET camera. CONCLUSIONS: These results demonstrate the ability of the PIXSIC device to record reliably the binding of PET ligands, with a high spatiotemporal resolution in anesthetized rodents. These first in vivo results are a key stage on the path to its implementation in awake freely moving animals.
PURPOSE: The aim of this work was to demonstrate the pharmacokinetic potential of a wireless pixelated β(+)-sensitive probe (PIXSIC). PROCEDURES: The binding of 2'-methoxyphenyl-(N-2'-pyridinyl)-p-[(18)F]fluoro-benzamidoethylpiperazine ([(18)F]MPPF), a 5-HT1A serotonin receptor radiopharmaceutical, was measured in anesthetized rats and compared to microPET data. The effects of a 5-HT1A antagonist injection on in vivo [(18)F]MPPF binding were monitored by PIXSIC. RESULTS: PIXSIC allowed differentiating the radioactive kinetics according to the location of its pixels in the hippocampus, cortex, corpus callosum, and cerebellum. The device accurately detected the changes in [(18)F]MPPF binding, after 5-HT1A antagonist blockade. The time-activity curves were reproducible and consistent with kinetics obtained simultaneously with a microPET camera. CONCLUSIONS: These results demonstrate the ability of the PIXSIC device to record reliably the binding of PET ligands, with a high spatiotemporal resolution in anesthetized rodents. These first in vivo results are a key stage on the path to its implementation in awake freely moving animals.
Authors: L Zimmer; F Pain; G Mauger; A Plenevaux; D Le Bars; R Mastrippolito; J F Pujol; B Renaud; P Lanièce Journal: Eur J Nucl Med Mol Imaging Date: 2002-07-25 Impact factor: 9.236
Authors: J Märk; D Benoit; L Balasse; M Benoit; J C Clémens; S Fieux; D Fougeron; J Graber-Bolis; B Janvier; M Jevaud; A Genoux; P Gisquet-Verrier; M Menouni; F Pain; L Pinot; C Tourvielle; L Zimmer; C Morel; P Laniece Journal: Phys Med Biol Date: 2013-06-13 Impact factor: 3.609
Authors: Tobi A Szuts; Vitaliy Fadeyev; Sergei Kachiguine; Alexander Sher; Matthew V Grivich; Margarida Agrochão; Pawel Hottowy; Wladyslaw Dabrowski; Evgueniy V Lubenov; Athanassios G Siapas; Naoshige Uchida; Alan M Litke; Markus Meister Journal: Nat Neurosci Date: 2011-01-16 Impact factor: 24.884
Authors: Matthias T Wyss; Nicolas M Obrist; Florent Haiss; Rolf Eckert; Ross Stanley; Cyrill Burger; Alfred Buck; Bruno Weber Journal: Neuroimage Date: 2009-07-08 Impact factor: 6.556