11C-choline vs. 18F-FDG PET/CT in assessing bone involvement in patients with multiple myeloma.

BACKGROUND: Multiple Myeloma (MM) is a B cell neoplasm causing lytic or osteopenic bone abnormalities. Whole body skeletal survey (WBSS), Magnetic resonance (MR) and 18F-FDG PET/CT are imaging techniques routinely used for the evaluation of bone involvement in MM patients. AIM: As MM bone lesions may present low 18F-FDG uptake; the aim of this study was to assess the possible added value and limitations of 11C-Choline to that of 18F-FDG PET/CT in patients affected with MM.
METHODS: Ten patients affected with MM underwent a standard 11C-Choline PET/CT and an 18F-FDG PET/CT within one week. The results of the two scans were compared in terms of number, sites and SUVmax of lesions.
RESULTS: Four patients (40%) had a negative concordant 11C-Choline and 18F-FDG PET/CT scans. Two patients (20%) had a positive 11C-Choline and 18F-FDG PET/CT scans that identified the same number and sites of bone lesions. The remaining four patients (40%) had a positive 11C-Choline and 18F-FDG PET/CT scan, but the two exams identified different number of lesions. Choline showed a mean SUVmax of 5 while FDG showed a mean SUVmax of 3.8 (P = 0.042). Overall, 11C-Choline PET/CT scans detected 37 bone lesions and 18F-FDG PET/CT scans detected 22 bone lesions but the difference was not significant (P = 0.8).
CONCLUSION: According to these preliminary data, 11C-Choline PET/CT appears to be more sensitive than 18F-FDG PET/CT for the detection of bony myelomatous lesions. If these data are confirmed in larger series of patients, 11C-Choline may be considered a more appropriate functional imaging in association with MRI for MM bone staging.

Background

Multiple myeloma (MM) is a B cell neoplasm involving bones in more than 80% of cases. Patients frequently present with a single or multiple lytic bone lesions causing bone pain, pathological fractures and hypercalcaemia [1-5]. Bone abnormalities (lytic or osteopenic) are one of the myeloma related organ dysfunction [6] and are responsible for low quality of life due to severe pain and high incidence of fractures, and this is particularly dangerous if located in the spine. The incidence of vertebral fractures can be reduced with bisphosphonates that are now available in the therapeutic armamentarium of MM.

Bone lesions are usually evaluated with a spectrum of imaging techniques, among which whole body skeletal survey (WBSS) and spine and pelvis Magnetic Resonance Imaging (MRI) are the most widely used. [7].

WBSS is known to be relatively insensitive for bone damage detection as only those lesions characterized by a high re-absorption rate (therefore appearing at a late stage) are visible. Furthermore, WBSS, being a planar technique, can easily underestimate bone involvement especially within the spine where overlying tissues and rib cage hinder the assessment of osteolysis. In addition, WBSS cannot distinguish between idiopathic osteoporotic vertebral fractures and fractures due to MM and is not suitable to assess the response to therapy.

Spine MRI, which was recently integrated in the Durie and Salmon PLUS staging system, is proved to have a very good sensitivity compared to WBSS especially at disease onset [8-13]. The main limitations of MRI are the inability to perform the scan in the presence of metallic prosthesis or in case of severe claustrophobia. More importantly, MRI is limited by the partial field of view that includes only the spine and the pelvis. The skull, femura, humeri, clavicles and ribs are often affected by lytic lesions but are not included in MRI field of view. Whole Body MRI is now available for a complete skeletal survey, but is rarely employed on a routine basis.

Nuclear medicine imaging techniques were also used to assess MM bone involvement. 99mTc-diphosphonate bone scan and 67Ga-citrate scan were found to be unreliable due to minimal osteoblastic activity and hypovascularity of lesions. 99mTc-Sestamibi whole-body scan is more accurate but the low spatial resolution limits the identification of small lesions. Furthermore, the image interpretation can be difficult due to the low tracer uptake within the lesions and to the high physiological liver uptake that can mask vertebral and right rib lesions. Therefore, nuclear medicine tests have not gained widespread acceptance [14-20].

In recent years, 18F-FDG PET and PET/CT were used as possible novel strategy for MM evaluation. 18F-FDG PET is a total body imaging technique that can detect both medullary and extra-medullary lesions and has been found useful for improving staging accuracy. Durie et al. in 2002 demonstrated that a negative 18F-FDG PET scan predicts stable monoclonal gammopathy of indeterminate significance (MGIS), identifies small lesions not detected by WBSS, identifies extra-medullary lesions related to poor prognosis and predicts an early relapse if it was positive after therapy [21]. These results were confirmed by other recent publications [22,23].

As stated before, 18F-FDG PET/CT is useful to correctly stage MM with increased accuracy of bone lesion detection at disease onset. It is more sensitive than WBSS and includes all the bones located out of the MRI field of view [24].

Despite its sensitivity, the uptake of FDG assessed with the maximum Standardized Uptake Value (SUVmax) can be very low, sometimes even comparable to the SUVmax of a benign lesion. Distinguishing between a benign lesion and a low-metabolic MM lesion can therefore be difficult to achieve.

11C-Choline is a radiolabelled PET tracer compound that is clinically used for the evaluation of relapse of prostate cancer. As with MM, prostate cancer does not show a significant increase of 18F-FDG uptake, but is characterized by a high 11C-Choline uptake [25]. Interestingly, a recently published case report has shown increased 11C-Choline uptake in a solitary plasmacytoma of bones [26].

The aim of our study was to assess the possible added value and limitations of 11C-Choline compared with 18F-FDG PET/CT in patients affected by MM.

Patients and methods

Between November 2004 and June 2006, we studied 10 patients (7 males and 3 females, mean age 58 years) affected with MM. They underwent 11C-Choline PET/CT and 18F-FDG PET/CT within one week (in most cases on the same day). Four of the patients were evaluated at completion of initial therapy, 2 during follow-up and 4 at disease relapse. At disease onset, all the patients were in Durie and Salmon stage III due to the presence of bone lesions. For the 11C-Choline scan, all patients provided informed consent for participation and anonymous publication of data.

Patients were injected with 5.3 MBq/Kg 11C-Choline iv and scanned after an uptake period of 5 minutes. Data acquisition was performed with a dedicated PET/CT tomograph (GE, Discovery). Images were acquired in 2D mode for 4 min per bed position, and attenuation correction was performed with a CT-based method (120 kV, 80 mA). Each PET/CT scan was read by two nuclear medicine physicians and the reports agreed upon by consensus. Each visible area of focal 11C-Choline uptake in bone (excluding joints) was considered positive for a myelomatous lesion. The SUVmax was calculated using the following formula:

Tissue concentration (MBq/g)/injected dose (MBq)/body weight (g)

At least 4 hours after the 11C-Choline scan, the patients were injected with 5.3 MBq/Kg 18F-FDG iv. None of the patients was diabetic and the fasting time required for 11F-FDG studies was at least 4 hours. The uptake time was 60–90 minutes and the data acquisition was performed as for the 11C-Choline scan.

11C-Choline scan results were compared to 18F-FDG scan results in terms of number of lesions and SUVmax. The SUVmax cut-off was 1.0 for 11C-Choline studies (the higher uptake that we measured in normal bones) while all the areas of focal uptake were interpreted as positive for myeloma in 18F-FDG scan unless they were at sites of known accumulation. The latter include the kidneys and bladder, gastrointestinal tract, and skeletal areas showing symmetric joint uptake, especially within the shoulder girdle [21]. A mild diffuse increase in bone marrow activity was not interpreted as positive for myeloma as it is a frequent finding even in normal patients [27].

The CT attenuation correction map was not used as a reference diagnostic tool. Several bone lesions are normally detected by PET at an early stage before these ere detectable with morphological imaging such as CT since density alteration occurs much later than metabolic activity. Furthermore, patients evaluated after being treated with a specific therapy may present with persistent osteolytic lesions on CT that do not show significant metabolic activity any more.

All patients had at least one-year follow-up and underwent several imaging procedures according to the clinical decision and needs.

Statistical analysis

Statistical significance of differences in 11C-Choline SUVmax and 18F-FDG SUVmax was determined using the Student t Test. A two-tailed Mann-Whitney test was used to compare the number of lesions detected with 11C-Choline and 18F-FDG. The minimal level of significance was a P < 0.05.

Results

The mean number of lesions detected per patient in the entire group was 3.7 for 11C-Choline and 2.2 for 18F-FDG (P = 0.8). Considering only positive patients, the mean number of lesions detected per patient was 7.4 for 11C-Choline and 3.7 for 18F-FDG (Table 1).

Table 1

Number of bone lesions detected by 11C-Choline PET/CT and 18F-FDG PET/CT patient by patient.

Patient	11C-Choline PET/CT	18F-FDG PET/CT
1	0	0
2	8	1
3	0	0
4	2	1
5	0	0
6	11	11
7	10	2
8	0	0
9	6	6
10	0	1
Total	37	22

In 4/10 patients (40%) there was a negative concordant 11C-Choline and 18F-FDG PET/CT scans. These findings were consistent with clinical, laboratory and radiological data indicating a complete remission at the time of imaging. Of those four patients, three were evaluated after therapy and one during follow-up.

In 2/10 patients (20%), evaluation was performed due to suspicion of disease relapse and both 11C-Choline and 11F-FDG PET/CT scans were positive. In this group, both techniques identified the same number and sites of bone lesions.

The remaining 4/10 (40%) patients had a positive 11C-Choline and 18F-FDG PET/CT scans, but the two techniques identified a different number of lesions. In 3/4 patients, 11C-Choline identified more lesions compared to 18F-FDG (8 vs. 1; 2 vs. 1; 10 vs. 2), while in 1/4 patient 18F-FDG detected a disease relapse within the pelvis that was negative with 11C-Choline. Of these four patients, 2/4 were evaluated due to suspicion of disease relapse, 1/4 following therapy and 1/4 during follow-up.

Table 2 shows the SUV max on a lesion by lesion basis for 11C-Choline scans and 18F-FDG scans (Table 2). 11C-Choline showed a mean SUVmax of 5, while 18F-FDG showed a mean SUVmax of 3.8 and the difference was statistically significant (p = .042). The SUVmax of visually detectable lesions ranged from 1.1 to 19.2 for 11C-Choline and from 2 to 13.7 for 18F-FDG. (Table 2).

Table 2

Sites of lesions and SUVmax (11C-Choline and 18F-FDG) on a lesion by lesion basis. Bold: SUVmax of positive lesions. Non Bold: SUVmax of negative areas.

Patient number	Gender	Age (years)	Disease stage	Therapy	Indication to PET	Follow-up (months)	Confirmation of lesions	Number of lesions	Site of lesions	SUVmax Choline PET	SUVmax FDG PET
1	Male	61	IgA/lamda IIIA	Chemotherapy + double autotranspalnt	Post-therapy	19	Clinical follow-up	0
2	Male	55	IgA/lamba IIIA	Chemotherapy + autotranspalnt	Suspect relapse	20	Clinical follow-up	12345678	pleurasoft tissueshumerushumeral headsoft tissuesRibsD11D12	7.04.25.16.03.04.72.52.7	1.02.01.31.90.81.51.01.0
3	Male	56	IgA/lamba IIIA	Chemotherapy + autotranspalnt	Post-therapy	8	Whole body X-rays		0
4	Male	72	Solitary plasmacytoma of bones	Radiotherapy	Follow-up	31	Magnetic resonance imaging	910	sacrumD8–D10	5.62.0	2.81.0
5	Male	62	IgG/K IA	Chemotherapy + autotranspalnt	Follow-up	16	Clinical follow-up	0
6	Female	55	IgG/lamba IIIA	Chemo-radiotherapy	Suspect relapse	16	Clinical follow-up	1112131415161718192021	skullscapulaclaviclehumerussoft tissuessoft tissuesribssternumpelvissacrumfemur	1.34.22.83.65.54.56.54.07.56.05.7	3.59.76.64.513.712.36.53.04.95.83.1
7	Female	57	IgG/lamba IIIA	Chemotherapy + autotranspalnt	Suspect relapse	16	FDG PET/CT	22232425262728293031	scapulasternumclavicleribsD4–D8L4L5pelvissacrumfemur	1.31.82.43.02.22.32.22.91.13.0	1.03.41.22.51.01.71.90.91.61.7
8	Male	59	IgG/lamba IA	Chemotherapy	Post-therapy	8	FDG PET/CT	0
9	Female	49	IgA/K IIIA	Chemotherapy + autotranspalnt	Suspect relapse	8	FDG PET/CT	323334353637	skullclaviclescapularibspelvisfemur	15.012.97.512.44.619.2	5.89.12.58.53.07.6
10	Male	53	Solitary plasmacytoma of bones	Radiotherapy	Post-therapy	1	Magnetic resonance imaging	38	sacrum	0.9	4.9
									Mean	5.0	3.8
									p	0.042

Overall, 11C-Choline PET/CT scans detected 37 bone lesions while 18F-FDG PET/CT scans detected 22 bone lesions. This difference, however, was not statistically significant and the P value was 0.8.

All the patients underwent a follow-up (1 month to 1 year long) by repeating 18F-FDG PET/CT, MRI or CT. No false positive findings were observed for the 18F-FDG or 11C-Choline.

Discussion

Our preliminary results show that 11C-Choline PET/CT detected more myelomatous lesions than 18F-FDG PET/CT in our group of 10 patients. Although the difference between the two tracers was not statistically significant in terms of mean number of lesions detected, it is interesting to note that 11C-Choline detected more lesions than 18F-FDG in patient 2 and 7 (8 vs. 1 and 10 vs. 2), radically changing these patients' management.

One patient turned out positive for several pleural lesions, soft tissue involvement and bone lesions on 11C-Choline, while 18F-FDG detected only the soft tissue lesion (Figure 1). Another patient turned out positive for several bone lesions on 11C-Choline scan, while 18F-FDG detected only a rib and a sternal lesion. On average, SUVmax was significantly higher for 11C-Choline-positive lesions compared to 11F-FDG-positive lesions (5.0 vs. 3.8), and this is an unusual finding as 11C-Choline and 18F-FDG-positive lesions behave in different ways.

Figure 1

18F-FDG PET scan (A) and 11C-Choline PET scan (B) of a MM patient. A) a small area of minimal uptake is detectable in dorsal soft tissues (black arrow), B) several areas of increased uptake are detected in bones, soft tissues and pleura (black arrows).

It is not clear why myelomatous lesions demonstrate such high 11C-Choline uptake compared to 18F-FDG. Sasagawa et al, in a series of 16 patients affected by MM, demonstrated that the serum levels of lysophospholipids were significantly increased compared to normal patients [28]. Recently, Hideshima et al. showed that perifosine, an alkylphospholipid, is active in-vitro against myelomatous cells by inhibiting the phosphatidilinositol 3-kinase/Akt, a mitogen-activated protein kinase which mediates MM cell resistance to conventional therapies [29].

These data suggest that phospholipids are strongly involved in the metabolism of myelomatous cells, especially in the modulation of intracellular growth signal transduction pathways.

Choline is a small molecule precursor of phospholipids and its uptake is increased in proliferating cells because it is involved in membrane metabolism and growth (increased during the mitotic process) that is significantly altered in MM lesions.

The additional value of sensitive bone imaging techniques in patients affected with MM is still not well defined, but remains part of the routine assessment of disease activity. However, recent studies suggest that the number of bone lesions is related to the prognosis and that the functional measurement of reduction in metabolism is a long term predictive parameter of therapy response [30].

If this concept is confirmed in studies with larger number of patients, the role of a sensitive technique that assesses the whole body, such as 11C-Choline, could acquire importance in patients affected by MM. In particular, it may help to customise an early aggressive therapy in case of multiple bone lesions to prevent a disease relapse or loss of bone mineral density resulting in multiple fractures.

The main disadvantage of 11C-Choline is the physiological liver uptake that prevents detection of hepatic lesions that may occur, though rarely, in MM patients. Furthermore, the role of 11C-Choline PET for the detection of infiltrative pattern of the spine, not characterized by distinct focal lesions, needs further assessment as our small series did not include any patient with such pattern on MRI. This may prove to be useful as 18F-FDG PET is not sensitive in the identification of infiltrative pattern of the spine.

One patient had a positive 18F-FDG PET scan showing a focal area of increased uptake located in the pelvis and a negative 11C-Choline PET scan (Figure 2). The significance of this mismatch is difficult to assess due to the short period of follow up. It has been suggested that a lesion with an initially negative 18F-FDG scan that shows uptake at a later stage may have developed de-differentiation of cancer cells and this may explain this discordant finding. The prognosis in such cases is inversely correlated with the SUVmax [31,32].

Figure 2

18F-FDG PET scan (A) a small area of minimal uptake is detectable in the pelvis (black arrow) B) 11C-Choline PET scan of a MM patient. no Choline uptake is shown.

Conclusion

According to our preliminary data, 11C-Choline PET/CT appears to be more sensitive than 18F-FDG PET/CT for the detection of bone myelomatous lesions. If these data can be confirmed in a larger series of patients, 11C-Choline could be the most appropriate functional imaging in combination with MRI for MM bone staging.

Competing interests

The author(s) declare that they have no competing interests.

Authors' contributions

EZ, MC, PT managed the patients and performed clinical and biochemical examinations. CN, RF, VA participated in the design of the study. CP performed the quality controls of the PET/CT system. MF, PC, GCM performed the PET/CT examinations and drafted the manuscript. SF, DR, AA participated to the coordination of the study and helped to draft the study. All authors read and approved the final manuscript.