| Literature DB >> 17401337 |
Christine B Karim1, Zhiwen Zhang, David D Thomas.
Abstract
A procedure is described for the synthetic incorporation into membrane proteins of the non-natural amino acid TOAC (2,2,6,6-tetramethyl-piperidine-1-oxyl-4-amino-4-carboxylic acid), which is coupled rigidly to the alpha-carbon, providing direct detection of peptide backbone dynamics by electron paramagnetic resonance (EPR). Also included is a protocol for the functional reconstitution of the spin-labeled protein in lipid vesicles. This protocol can be completed in 17 d.Entities:
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Year: 2007 PMID： 17401337 DOI： 10.1038/nprot.2007.2
Source DB: PubMed Journal: Nat Protoc ISSN： 1750-2799 Impact factor: 13.491