Literature DB >> 17400773

Development of high-throughput phenotyping of metagenomic clones from the human gut microbiome for modulation of eukaryotic cell growth.

Karine Gloux1, Marion Leclerc, Harout Iliozer, René L'Haridon, Chaysavanh Manichanh, Gérard Corthier, Renaud Nalin, Hervé M Blottière, Joël Doré.   

Abstract

Metagenomic libraries derived from human intestinal microbiota (20,725 clones) were screened for epithelial cell growth modulation. Modulatory clones belonging to the four phyla represented among the metagenomic libraries were identified (hit rate, 0.04 to 8.7% depending on the screening cutoff). Several candidate loci were identified by transposon mutagenesis and subcloning.

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Year:  2007        PMID： 17400773      PMCID： PMC1932692          DOI： 10.1128/AEM.02204-06

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  31 in total

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  11 in total

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