Literature DB >> 1738596

PCR using a thermostable polymerase with 3' to 5' exonuclease activity generates blunt products suitable for direct cloning.

C J Lohff1, K B Cease.   

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Year:  1992        PMID： 1738596      PMCID： PMC310344          DOI： 10.1093/nar/20.1.144

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


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  7 in total

1.  Construction of T-vectors, a rapid and general system for direct cloning of unmodified PCR products.

Authors:  D Marchuk; M Drumm; A Saulino; F S Collins
Journal:  Nucleic Acids Res       Date:  1991-03-11       Impact factor: 16.971

2.  A simple and efficient method for direct cloning of PCR products using ddT-tailed vectors.

Authors:  T A Holton; M W Graham
Journal:  Nucleic Acids Res       Date:  1991-03-11       Impact factor: 16.971

3.  The polymerase chain reaction colony miniprep.

Authors:  L I Zon; D M Dorfman; S H Orkin
Journal:  Biotechniques       Date:  1989 Jul-Aug       Impact factor: 1.993

4.  Efficient cloning of PCR generated DNA containing terminal restriction endonuclease recognition sites.

Authors:  V Jung; S B Pestka; S Pestka
Journal:  Nucleic Acids Res       Date:  1990-10-25       Impact factor: 16.971

5.  A simple method for site-directed mutagenesis using the polymerase chain reaction.

Authors:  A Hemsley; N Arnheim; M D Toney; G Cortopassi; D J Galas
Journal:  Nucleic Acids Res       Date:  1989-08-25       Impact factor: 16.971

6.  Novel non-templated nucleotide addition reactions catalyzed by procaryotic and eucaryotic DNA polymerases.

Authors:  J M Clark
Journal:  Nucleic Acids Res       Date:  1988-10-25       Impact factor: 16.971

7.  Molecular cloning and characterization of the HTLV-III virus associated with AIDS.

Authors:  B H Hahn; G M Shaw; S K Arya; M Popovic; R C Gallo; F Wong-Staal
Journal:  Nature       Date:  1984 Nov 8-14       Impact factor: 49.962
  7 in total
  7 in total

1.  Perils of gene mapping with microsatellite markers.

Authors:  J A Knowles; V J Vieland; T C Gilliam
Journal:  Am J Hum Genet       Date:  1992-10       Impact factor: 11.025

2.  The signal recognition particle (SRP) database.

Authors:  C Zwieb; N Larsen
Journal:  Nucleic Acids Res       Date:  1992-05-11       Impact factor: 16.971

3.  Phosphorothioate primers improve the amplification of DNA sequences by DNA polymerases with proofreading activity.

Authors:  A Skerra
Journal:  Nucleic Acids Res       Date:  1992-07-25       Impact factor: 16.971

4.  Factors affecting fidelity of DNA synthesis during PCR amplification of d(C-A)n.d(G-T)n microsatellite repeats.

Authors:  J M Hite; K A Eckert; K C Cheng
Journal:  Nucleic Acids Res       Date:  1996-06-15       Impact factor: 16.971

5.  Efficient One-Step Fusion PCR Based on Dual-Asymmetric Primers and Two-Step Annealing.

Authors:  Yilan Liu; Jinjin Chen; Anders Thygesen
Journal:  Mol Biotechnol       Date:  2018-02       Impact factor: 2.695

6.  Expression of mouse phosphofructokinase-M gene alternative transcripts: evidence for the conserved two-promoter system.

Authors:  H Nakajima; T Noguchi; T Hamaguchi; K Tomita; T Hanafusa; N Kono; T Tanaka; M Kuwajima; Y Matsuzawa
Journal:  Biochem J       Date:  1994-10-15       Impact factor: 3.857

7.  TA-GC cloning: A new simple and versatile technique for the directional cloning of PCR products for recombinant protein expression.

Authors:  Athanasios Niarchos; Anastasia Siora; Evangelia Konstantinou; Vasiliki Kalampoki; George Lagoumintzis; Konstantinos Poulas
Journal:  PLoS One       Date:  2017-11-01       Impact factor: 3.240
  7 in total

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