Literature DB >> 2020554

A simple and efficient method for direct cloning of PCR products using ddT-tailed vectors.

T A Holton1, M W Graham.   

Abstract

Mesh:

Substances:

Year:  1991        PMID: 2020554      PMCID: PMC333802          DOI: 10.1093/nar/19.5.1156

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN: 0305-1048            Impact factor:   16.971


× No keyword cloud information.
  2 in total

1.  Restriction endonuclease cleavage at the termini of PCR products.

Authors:  D L Kaufman; G A Evans
Journal:  Biotechniques       Date:  1990-09       Impact factor: 1.993

2.  Novel non-templated nucleotide addition reactions catalyzed by procaryotic and eucaryotic DNA polymerases.

Authors:  J M Clark
Journal:  Nucleic Acids Res       Date:  1988-10-25       Impact factor: 16.971

  2 in total
  98 in total

1.  Plasmid-encoded metallo-beta-lactamase (IMP-6) conferring resistance to carbapenems, especially meropenem.

Authors:  H Yano; A Kuga; R Okamoto; H Kitasato; T Kobayashi; M Inoue
Journal:  Antimicrob Agents Chemother       Date:  2001-05       Impact factor: 5.191

2.  The phenylalanine ammonia-lyase gene family in raspberry. Structure, expression, and evolution.

Authors:  A Kumar; B E Ellis
Journal:  Plant Physiol       Date:  2001-09       Impact factor: 8.340

3.  Entamoeba histolytica calreticulin: an endoplasmic reticulum protein expressed by trophozoites into experimentally induced amoebic liver abscesses.

Authors:  Enrique González; Maria del Carmen García de Leon; Isaura Meza; Rodolfo Ocadiz-Delgado; Patricio Gariglio; Angelica Silva-Olivares; Silvia Galindo-Gómez; Mineko Shibayama; Patricia Morán; Alicia Valadez; Angelica Limón; Liliana Rojas; Eric G Hernández; René Cerritos; Cecilia Ximenez
Journal:  Parasitol Res       Date:  2010-10-05       Impact factor: 2.289

4.  The enigmatic Escherichia coli fadE gene is yafH.

Authors:  John W Campbell; John E Cronan
Journal:  J Bacteriol       Date:  2002-07       Impact factor: 3.490

5.  The PRESAT-vector: asymmetric T-vector for high-throughput screening of soluble protein domains for structural proteomics.

Authors:  Natsuko Goda; Takeshi Tenno; Hirotoshi Takasu; Hidekazu Hiroaki; Masahiro Shirakawa
Journal:  Protein Sci       Date:  2004-03       Impact factor: 6.725

6.  pSDTV vector: a modification of the pBluescript SK+ plasmid in order to perform PCR-fragments TA-cloning using Eam1105I restriction endonuclease.

Authors:  Svetoslav G Dimov
Journal:  Mol Biol Rep       Date:  2011-12-28       Impact factor: 2.316

7.  pYEMF, a pUC18-derived XcmI T-vector for efficient cloning of PCR products.

Authors:  Jingsong Gu; Chunjiang Ye
Journal:  Mol Biotechnol       Date:  2011-03       Impact factor: 2.695

8.  Variants in an Hdac9 intronic enhancer plasmid impact Twist1 expression in vitro.

Authors:  Tyler E Siekmann; Madelyn M Gerber; Amanda Ewart Toland
Journal:  Mamm Genome       Date:  2015-12-31       Impact factor: 2.957

9.  Construction of a lentiviral T/A vector for direct analysis of PCR-amplified promoters.

Authors:  Fu-xian Yu; Zhi-wei Zhu; Xiao-yu Chen; Jing Huang; Tuan-yuan Shi; Jun-xing Li; Jian-zhi Pan
Journal:  Mol Biol Rep       Date:  2014-08-05       Impact factor: 2.316

10.  The Caulobacter crescentus paracrystalline S-layer protein is secreted by an ABC transporter (type I) secretion apparatus.

Authors:  P Awram; J Smit
Journal:  J Bacteriol       Date:  1998-06       Impact factor: 3.490

View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.