Literature DB >> 1733862

Walking, cloning, and mapping with yeast artificial chromosomes: a contig encompassing D21S13 and D21S16.

R Butler1, D J Ogilvie, P Elvin, J H Riley, R S Finniear, G Slynn, J E Morten, A F Markham, R Anand.   

Abstract

Chromosome 21 has often been used as a model system for the development of genome mapping and cloning strategies in humans. In this report methods for systematic chromosome walking, cloning, and mapping are exemplified in the construction of a 1.5-Mb yeast artificial chromosome (YAC) contig encompassing and extending 400 kb beyond each of the genetic loci D21S13 and D21S16. Isolation of insert-terminal sequences from YACs in this contig provides a set of closely spaced physical markers. These have been used to generate a long-range genomic restriction map.

Entities:  

Mesh：

Substances：

Year:  1992        PMID： 1733862     DOI： 10.1016/0888-7543(92)90404-g

Source DB:  PubMed          Journal:  Genomics        ISSN： 0888-7543            Impact factor:   5.736


  5 in total

1.  Rapid mapping of markers applying vectorette technology to YAC fragmentation allows easy assembly of a high-density STS bacterial clone contig spanning the markers D6S1260-D6S1918.

Authors:  J D Shearman; J J Pointon; A T Merryweather-Clarke; C Stone; S W Horsley; L Kearney; W M Rosenberg; K J Robson
Journal:  Mamm Genome       Date:  1998-03       Impact factor: 2.957

2.  Construction of a YAC contig and an STS map spanning 3.6 megabase pairs in Xp22.1.

Authors:  D Trump; G Pilia; P H Dixon; C Wooding; R Thakrar; S E Leigh; R Nagaraja; M P Whyte; D Schlessinger; R V Thakker
Journal:  Hum Genet       Date:  1996-01       Impact factor: 4.132

3.  A simple and efficient method for constructing high resolution physical maps.

Authors:  K Yoshida; M P Strathmann; C A Mayeda; C H Martin; M J Palazzolo
Journal:  Nucleic Acids Res       Date:  1993-07-25       Impact factor: 16.971

4.  A YAC contig encompassing the Treacher Collins syndrome critical region at 5q31.3-32.

Authors:  J Dixon; A J Gladwin; S K Loftus; J H Riley; R Perveen; J J Wasmuth; R Anand; M J Dixon
Journal:  Am J Hum Genet       Date:  1994-08       Impact factor: 11.025

5.  Analysis of chromosome 21 yeast artificial chromosome (YAC) clones.

Authors:  F Tassone; S Cheng; K Gardiner
Journal:  Am J Hum Genet       Date:  1992-12       Impact factor: 11.025
  5 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.