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Literature DB >> 17122397

Comparison of primer sets for use in automated ribosomal intergenic spacer analysis of aquatic bacterial communities: an ecological perspective.

Stuart E Jones¹, Ashley L Shade, Katherine D McMahon, Angela D Kent.

Abstract

Two primer sets for automated ribosomal intergenic spacer analysis (ARISA) were used to assess the bacterial community composition (BCC) in Lake Mendota, Wisconsin, over 3 years. Correspondence analysis revealed differences in community profiles generated by different primer sets, but overall ecological patterns were conserved in each case. ARISA is a powerful tool for evaluating BCC change through space and time, regardless of the specific primer set used.

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Year: 2006 PMID： 17122397 PMCID： PMC1796982 DOI： 10.1128/AEM.02130-06

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

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