Literature DB >> 17077492

Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of DapA (Rv2753c) from Mycobacterium tuberculosis.

Georgia Kefala1, Manfred S Weiss.   

Abstract

Dihydrodipicolinate synthase from Mycobacterium tuberculosis (DHDPS, DapA, Rv2753c) has been cloned, heterologously expressed in Escherichia coli, purified using standard chromatographic techniques and crystallized in a monoclinic crystal form. Preliminary diffraction data analysis suggests the presence of two independent tetramers in the asymmetric unit in almost the same relative orientation.

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Year:  2006        PMID： 17077492      PMCID： PMC2225217          DOI： 10.1107/S1744309106039844

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  11 in total

1.  SFCHECK: a unified set of procedures for evaluating the quality of macromolecular structure-factor data and their agreement with the atomic model.

Authors:  A A Vaguine; J Richelle; S J Wodak
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  1999-01-01

Review 2.  Inhibitors of lysine biosynthesis as antibacterial agents.

Authors:  Craig A Hutton; Timothy J Southwood; Jennifer J Turner
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Journal:  J Gen Microbiol       Date:  1956-07

4.  The CCP4 suite: programs for protein crystallography.

Authors: 
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  1994-09-01

5.  Solvent content of protein crystals.

Authors:  B W Matthews
Journal:  J Mol Biol       Date:  1968-04-28       Impact factor: 5.469

Review 6.  Variation in protection by BCG: implications of and for heterologous immunity.

Authors:  P E Fine
Journal:  Lancet       Date:  1995-11-18       Impact factor: 79.321

7.  Structure of dihydrodipicolinate synthase of Nicotiana sylvestris reveals novel quaternary structure.

Authors:  S Blickling; H G Beisel; D Bozic; J Knäblein; B Laber; R Huber
Journal:  J Mol Biol       Date:  1997-12-12       Impact factor: 5.469

8.  Reaction mechanism of Escherichia coli dihydrodipicolinate synthase investigated by X-ray crystallography and NMR spectroscopy.

Authors:  S Blickling; C Renner; B Laber; H D Pohlenz; T A Holak; R Huber
Journal:  Biochemistry       Date:  1997-01-07       Impact factor: 3.162

9.  Consensus statement. Global burden of tuberculosis: estimated incidence, prevalence, and mortality by country. WHO Global Surveillance and Monitoring Project.

Authors:  C Dye; S Scheele; P Dolin; V Pathania; M C Raviglione
Journal:  JAMA       Date:  1999-08-18       Impact factor: 56.272

10.  The crystal structure of dihydrodipicolinate synthase from Escherichia coli at 2.5 A resolution.

Authors:  C Mirwaldt; I Korndörfer; R Huber
Journal:  J Mol Biol       Date:  1995-02-10       Impact factor: 5.469
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  8 in total

1.  Cloning, expression, purification and crystallization of dihydrodipicolinate synthase from the psychrophile Shewanella benthica.

Authors:  Jacinta M Wubben; Con Dogovski; Renwick C J Dobson; Rachel Codd; Juliet A Gerrard; Michael W Parker; Matthew A Perugini
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2010-10-29

2.  The three-dimensional structure of diaminopimelate decarboxylase from Mycobacterium tuberculosis reveals a tetrameric enzyme organisation.

Authors:  Simone Weyand; Georgia Kefala; Dmitri I Svergun; Manfred S Weiss
Journal:  J Struct Funct Genomics       Date:  2009-06-19

3.  Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of tetrahydrodipicolinate-N-succinyltransferase (Rv1201c) from Mycobacterium tuberculosis.

Authors:  Linda Schuldt; Simone Weyand; Georgia Kefala; Manfred S Weiss
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2008-08-29

4.  Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of the regulatory domain of aspartokinase (Rv3709c) from Mycobacterium tuberculosis.

Authors:  Linda Schuldt; Ruth Suchowersky; Katharina Veith; Jochen Mueller-Dieckmann; Manfred S Weiss
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2011-02-25

5.  Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of succinyl-diaminopimelate desuccinylase (Rv1202, DapE) from Mycobacterium tuberculosis.

Authors:  Linda Reinhard; Jochen Mueller-Dieckmann; Manfred S Weiss
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2012-08-31

6.  The high-resolution structure of dihydrodipicolinate synthase from Escherichia coli bound to its first substrate, pyruvate.

Authors:  Sean R A Devenish; Juliet A Gerrard; Geoffrey B Jameson; Renwick C J Dobson
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2008-11-28

7.  Virtual Screening of potential drug-like inhibitors against Lysine/DAP pathway of Mycobacterium tuberculosis.

Authors:  Aarti Garg; Rupinder Tewari; Gajendra P S Raghava
Journal:  BMC Bioinformatics       Date:  2010-01-18       Impact factor: 3.169

8.  Inhibition of Mycobacterium tuberculosis dihydrodipicolinate synthase by alpha-ketopimelic acid and its other structural analogues.

Authors:  Priyanka Shrivastava; Vikas Navratna; Yumnam Silla; Rikeshwer P Dewangan; Atreyi Pramanik; Sarika Chaudhary; GeethaVani Rayasam; Anuradha Kumar; Balasubramanian Gopal; Srinivasan Ramachandran
Journal:  Sci Rep       Date:  2016-08-09       Impact factor: 4.379
  8 in total

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