Literature DB >> 16984173

Observation of heteronuclear overhauser effects confirms the 15N-1H dipolar relaxation mechanism in a crystalline protein.

Nicolas Giraud1, Julien Sein, Guido Pintacuda, Anja Böckmann, Anne Lesage, Martin Blackledge, Lyndon Emsley.   

Abstract

The observation of proton to nitrogen-15 heteronuclear Overhauser effects in the microcrystalline protein Crh is used to confirm that the principal mechanism of relaxation of amide nitrogens is due to the fluctuation of the N-H dipolar couplings caused by N-H bond dynamics. Our observations reveal the central role of water as the main source of proton magnetization, and we provide an analysis of the different pathways that could lead to the observed results.

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Year:  2006        PMID： 16984173     DOI： 10.1021/ja064037g

Source DB:  PubMed          Journal:  J Am Chem Soc        ISSN： 0002-7863            Impact factor:   15.419


  10 in total

1.  Solid-state protein-structure determination with proton-detected triple-resonance 3D magic-angle-spinning NMR spectroscopy.

Authors:  Donghua H Zhou; John J Shea; Andrew J Nieuwkoop; W Trent Franks; Benjamin J Wylie; Charles Mullen; Dennis Sandoz; Chad M Rienstra
Journal:  Angew Chem Int Ed Engl       Date:  2007       Impact factor: 15.336

2.  High-performance solvent suppression for proton detected solid-state NMR.

Authors:  Donghua H Zhou; Chad M Rienstra
Journal:  J Magn Reson       Date:  2008-02-01       Impact factor: 2.229

3.  Quantitative one- and two-dimensional 13C spectra of microcrystalline proteins with enhanced intensity.

Authors:  Rudra N Purusottam; Geoffrey Bodenhausen; Piotr Tekely
Journal:  J Biomol NMR       Date:  2013-06-29       Impact factor: 2.835

4.  Quantitative analysis of backbone motion in proteins using MAS solid-state NMR spectroscopy.

Authors:  Veniamin Chevelkov; Uwe Fink; Bernd Reif
Journal:  J Biomol NMR       Date:  2009-07-24       Impact factor: 2.835

5.  Site-specific analysis of heteronuclear Overhauser effects in microcrystalline proteins.

Authors:  Juan Miguel Lopez del Amo; Vipin Agarwal; Riddhiman Sarkar; Justin Porter; Sam Asami; Martin Rübbelke; Uwe Fink; Yi Xue; Oliver F Lange; Bernd Reif
Journal:  J Biomol NMR       Date:  2014-07-03       Impact factor: 2.835

Review 6.  1H-Detected Biomolecular NMR under Fast Magic-Angle Spinning.

Authors:  Tanguy Le Marchand; Tobias Schubeis; Marta Bonaccorsi; Piotr Paluch; Daniela Lalli; Andrew J Pell; Loren B Andreas; Kristaps Jaudzems; Jan Stanek; Guido Pintacuda
Journal:  Chem Rev       Date:  2022-05-10       Impact factor: 72.087

7.  Hybridizing cross-polarization with NOE or refocused-INEPT enhances the sensitivity of MAS NMR spectroscopy.

Authors:  Rongchun Zhang; Kamal H Mroue; Ayyalusamy Ramamoorthy
Journal:  J Magn Reson       Date:  2016-03-24       Impact factor: 2.229

8.  Accounting for the temperature dependence of 13C spin-lattice relaxation of methyl groups in the glycyl-alanyl-leucine model system under MAS with spin diffusion.

Authors:  Van C Phan; Elizabeth A Fry; Kurt W Zilm
Journal:  J Biomol NMR       Date:  2019-08-12       Impact factor: 2.835

Review 9.  Studying Dynamics by Magic-Angle Spinning Solid-State NMR Spectroscopy: Principles and Applications to Biomolecules.

Authors:  Paul Schanda; Matthias Ernst
Journal:  Prog Nucl Magn Reson Spectrosc       Date:  2016-02-15       Impact factor: 9.795

10.  Structural constraints for the Crh protein from solid-state NMR experiments.

Authors:  Carole Gardiennet; Antoine Loquet; Manuel Etzkorn; Henrike Heise; Marc Baldus; Anja Böckmann
Journal:  J Biomol NMR       Date:  2008-03-05       Impact factor: 2.835
  10 in total

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