Literature DB >> 16981439

HPLC method for quantification and characterization of cholesterol and its oxidation products in eggs.

Mônica R Mazalli1, Alexandra C H F Sawaya, Marcos N Eberlin, Neura Bragagnolo.   

Abstract

A new method was developed for the simultaneous determination of cholesterol and its oxidation products in eggs, using HPLC with UV and refractive index (RI) detectors, and HPLC interfaced with atmospheric pressure chemical ionization coupled to MS (HPLC-APCI-MS). The best conditions for direct saponification of the sample and extraction of the non-saponifiable material were defined using complete factorial designs with central points. The method showed accuracy and precision with a detection limit between 0.002 and 0.079 microg/g. The oxides cholest-5-ene-3beta,20alpha-diol and cholest-5-ene-3beta,25-diol identified by HPLC-UV-RI were not confirmed by HPLC-APCI-MS.

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Year:  2006        PMID： 16981439     DOI： 10.1007/s11745-006-5010-0

Source DB:  PubMed          Journal:  Lipids        ISSN： 0024-4201            Impact factor:   1.880


  8 in total

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Journal:  J Chromatogr A       Date:  2000-10-27       Impact factor: 4.759

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Journal:  Lipids       Date:  1989-07       Impact factor: 1.880

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Authors:  Klaus Raith; Christian Brenner; Hany Farwanah; Gerit Müller; Klaus Eder; Reinhard H H Neubert
Journal:  J Chromatogr A       Date:  2005-03-04       Impact factor: 4.759

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Authors:  P van de Bovenkamp; T G Kosmeijer-Schuil; M B Katan
Journal:  Lipids       Date:  1988-11       Impact factor: 1.880

Review 6.  Biological effects of oxysterols: current status.

Authors:  F Guardiola; R Codony; P B Addis; M Rafecas; J Boatella
Journal:  Food Chem Toxicol       Date:  1996-02       Impact factor: 6.023

7.  HTHQ (1-O-hexyl-2,3,5-trimethylhydroquinone), an anti-lipid-peroxidative compound: its chemical and biochemical characterizations.

Authors:  T Hino; S Kawanishi; H Yasui; S Oka; H Sakurai
Journal:  Biochim Biophys Acta       Date:  1998-09-16

8.  Free radical scavenging activity of fermented papaya preparation and its effect on lipid peroxide level and superoxide dismutase activity in iron-induced epileptic foci of rats.

Authors:  K Imao; H Wang; M Komatsu; M Hiramatsu
Journal:  Biochem Mol Biol Int       Date:  1998-06
  8 in total
  3 in total

1.  Amaranth oil increased fecal excretion of bile Acid but had no effect in reducing plasma cholesterol in hamsters.

Authors:  Luíla Ivini Andrade de Castro; Rosana Aparecida Manólio Soares; Paulo H N Saldiva; Roseli A Ferrari; Ana M R O Miguel; Claudia A S Almeida; José Alfredo Gomes Arêas
Journal:  Lipids       Date:  2013-03-02       Impact factor: 1.880

2.  Vitamin E absorption and kinetics in healthy women, as modulated by food and by fat, studied using 2 deuterium-labeled α-tocopherols in a 3-phase crossover design.

Authors:  Maret G Traber; Scott W Leonard; Ifechukwude Ebenuwa; Pierre-Christian Violet; Yu Wang; Mahtab Niyyati; Sebastian Padayatty; Hongbin Tu; Amber Courville; Shanna Bernstein; Jaewoo Choi; Robert Shamburek; Sheila Smith; Brian Head; Gerd Bobe; Rajasekhar Ramakrishnan; Mark Levine
Journal:  Am J Clin Nutr       Date:  2019-11-01       Impact factor: 7.045

3.  A validated LC-MS/MS assay for quantification of 24(S)-hydroxycholesterol in plasma and cerebrospinal fluid.

Authors:  Rohini Sidhu; Hui Jiang; Nicole Y Farhat; Nuria Carrillo-Carrasco; Myra Woolery; Elizabeth Ottinger; Forbes D Porter; Jean E Schaffer; Daniel S Ory; Xuntian Jiang
Journal:  J Lipid Res       Date:  2015-04-12       Impact factor: 5.922
  3 in total

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