| Literature DB >> 15844526 |
Klaus Raith1, Christian Brenner, Hany Farwanah, Gerit Müller, Klaus Eder, Reinhard H H Neubert.
Abstract
Cholesterol oxidation products (COPs) can be formed in the body or in animal foods from cholesterol during food processing. A new method for the extraction and quantification of cholesterol, 7-ketocholesterol, cholestane-3beta-5alpha-6beta-triol, 25-hydroxycholesterol, 5,6alpha-epoxycholesterol, and 7beta-hydroxycholesterol by means of reversed-phase LC/atmospheric pressure chemical ionization mass spectrometry is presented. A baseline separation of all COPs was achieved, allowing a separate quantification also for isobaric compounds. The limits of detection were 15-30 ng/mL, quantification was performed from 100 ng/mL to 10 microg/mL with RSD < 2%. The method was applied successfully to the determination of cholesterol and COPs in processed foods such as pork, beef, chicken, and egg.Entities:
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Year: 2005 PMID： 15844526 DOI： 10.1016/j.chroma.2004.12.053
Source DB: PubMed Journal: J Chromatogr A ISSN： 0021-9673 Impact factor: 4.759