Literature DB >> 16885315

Quantitative PCR method for sensitive detection of ruminant fecal pollution in freshwater and evaluation of this method in alpine karstic regions.

Georg H Reischer1, David C Kasper, Ralf Steinborn, Robert L Mach, Andreas H Farnleitner.   

Abstract

A quantitative TaqMan minor-groove binder real-time PCR assay was developed for the sensitive detection of a ruminant-specific genetic marker in fecal members of the phylum Bacteroidetes. The qualitative and quantitative detection limits determined were 6 and 20 marker copies per PCR, respectively. Tested ruminant feces contained an average of 4.1 x 10(9) marker equivalents per g, allowing the detection of 1.7 ng of feces per filter in fecal suspensions. The marker was detected in water samples from a karstic catchment area at levels matching a gradient from negligible to considerable ruminant fecal influence (from not detectable to 10(5) marker equivalents per liter).

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Year:  2006        PMID： 16885315      PMCID： PMC1538736          DOI： 10.1128/AEM.00364-06

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  28 in total

1.  Application of the 5' fluorogenic exonuclease assay (TaqMan) for quantitative ribosomal DNA and rRNA analysis in sediments.

Authors:  J R Stults; O Snoeyenbos-West; B Methe; D R Lovley; D P Chandler
Journal:  Appl Environ Microbiol       Date:  2001-06       Impact factor: 4.792

2.  Evaluation of a rapid, quantitative real-time PCR method for enumeration of pathogenic Candida cells in water.

Authors:  Nichole E Brinkman; Richard A Haugland; Larry J Wymer; Muruleedhara Byappanahalli; Richard L Whitman; Stephen J Vesper
Journal:  Appl Environ Microbiol       Date:  2003-03       Impact factor: 4.792

3.  Tiered approach for identification of a human fecal pollution source at a recreational beach: case study at Avalon Bay, Catalina Island, California.

Authors:  Alexandria B Boehm; Jed A Fuhrman; Robert D Mrse; Stanley B Grant
Journal:  Environ Sci Technol       Date:  2003-02-15       Impact factor: 9.028

4.  Application of a rapid method for identifying fecal pollution sources in a multi-use estuary.

Authors:  Anne E Bernhard; Thierry Goyard; Michael T Simonich; Katharine G Field
Journal:  Water Res       Date:  2003-02       Impact factor: 11.236

5.  Quantification of Fusarium graminearum in infected wheat by species specific real-time PCR applying a TaqMan Probe.

Authors:  G H Reischer; M Lemmens; A Farnleitner; A Adler; R L Mach
Journal:  J Microbiol Methods       Date:  2004-10       Impact factor: 2.363

6.  Application of polymerase chain reaction (PCR) and TaqMan PCR techniques to the detection and identification of Rhodococcus coprophilus in faecal samples.

Authors:  M G Savill; S R Murray; P Scholes; E W Maas; R E McCormick; E B Moore; B J Gilpin
Journal:  J Microbiol Methods       Date:  2001-12       Impact factor: 2.363

7.  Direct analysis of genes encoding 16S rRNA from complex communities reveals many novel molecular species within the human gut.

Authors:  A Suau; R Bonnet; M Sutren; J J Godon; G R Gibson; M D Collins; J Doré
Journal:  Appl Environ Microbiol       Date:  1999-11       Impact factor: 4.792

8.  The enumeration of Bacteroides fragilis group organisms from sewage and natural waters.

Authors:  K Allsop; D J Stickler
Journal:  J Appl Bacteriol       Date:  1984-02

Review 9.  Microbial source tracking: state of the science.

Authors:  Joyce M Simpson; Jorge W Santo Domingo; Donald J Reasoner
Journal:  Environ Sci Technol       Date:  2002-12-15       Impact factor: 9.028

10.  Real-time PCR for quantification of Giardia and Cryptosporidium in environmental water samples and sewage.

Authors:  Rebecca A Guy; Pierre Payment; Ulrich J Krull; Paul A Horgen
Journal:  Appl Environ Microbiol       Date:  2003-09       Impact factor: 4.792
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  48 in total

1.  Blautia and Prevotella sequences distinguish human and animal fecal pollution in Brazil surface waters.

Authors:  Amber M Koskey; Jenny C Fisher; A Murat Eren; Rafael Ponce-Terashima; Mitermayer G Reis; Ronald E Blanton; Sandra L McLellan
Journal:  Environ Microbiol Rep       Date:  2014-07-09       Impact factor: 3.541

2.  Occurrence of Host-Associated Fecal Markers on Child Hands, Household Soil, and Drinking Water in Rural Bangladeshi Households.

Authors:  Alexandria B Boehm; Dan Wang; Ayse Ercumen; Meghan Shea; Angela R Harris; Orin C Shanks; Catherine Kelty; Alvee Ahmed; Zahid Hayat Mahmud; Benjamin F Arnold; Claire Chase; Craig Kullmann; John M Colford; Stephen P Luby; Amy J Pickering
Journal:  Environ Sci Technol Lett       Date:  2016-11-08

3.  Microbial Source Tracking in Adjacent Karst Springs.

Authors:  Shoshanit Ohad; Dalit Vaizel-Ohayon; Meir Rom; Joseph Guttman; Diego Berger; Valeria Kravitz; Shlomo Pilo; Zohar Huberman; Yechezkel Kashi; Efrat Rorman
Journal:  Appl Environ Microbiol       Date:  2015-05-22       Impact factor: 4.792

4.  Microbial community analysis and identification of alternative host-specific fecal indicators in fecal and river water samples using pyrosequencing.

Authors:  Ju-Yong Jeong; Hee-Deung Park; Kyong-Hee Lee; Hang-Yeon Weon; Jong-Ok Ka
Journal:  J Microbiol       Date:  2011-09-02       Impact factor: 3.422

5.  Patterns of Host-Associated Fecal Indicators Driven by Hydrology, Precipitation, and Land Use Attributes in Great Lakes Watersheds.

Authors:  Deborah K Dila; Steven R Corsi; Peter L Lenaker; Austin K Baldwin; Melinda J Bootsma; Sandra L McLellan
Journal:  Environ Sci Technol       Date:  2018-09-27       Impact factor: 9.028

6.  Fecal Contamination on Produce from Wholesale and Retail Food Markets in Dhaka, Bangladesh.

Authors:  Angela R Harris; Mohammad Aminul Islam; Leanne Unicomb; Alexandria B Boehm; Stephen Luby; Jennifer Davis; Amy J Pickering
Journal:  Am J Trop Med Hyg       Date:  2018-01       Impact factor: 2.345

7.  Distribution and Differential Survival of Traditional and Alternative Indicators of Fecal Pollution at Freshwater Beaches.

Authors:  Danielle D Cloutier; Sandra L McLellan
Journal:  Appl Environ Microbiol       Date:  2017-02-01       Impact factor: 4.792

8.  Relative abundance of Bacteroides spp. in stools and wastewaters as determined by hierarchical oligonucleotide primer extension.

Authors:  Pei-Ying Hong; Jer-Horng Wu; Wen-Tso Liu
Journal:  Appl Environ Microbiol       Date:  2008-03-14       Impact factor: 4.792

9.  Discrimination of viable and dead fecal Bacteroidales bacteria by quantitative PCR with propidium monoazide.

Authors:  Sungwoo Bae; Stefan Wuertz
Journal:  Appl Environ Microbiol       Date:  2009-03-06       Impact factor: 4.792

10.  Identification of two GH18 chitinase family genes and their use as targets for detection of the crayfish-plague oomycete Aphanomyces astaci.

Authors:  Gerald Hochwimmer; Reinhard Tober; Renè Bibars-Reiter; Elisabeth Licek; Ralf Steinborn
Journal:  BMC Microbiol       Date:  2009-08-31       Impact factor: 3.605
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