Literature DB >> 16880560

Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of DapC (Rv0858c) from Mycobacterium tuberculosis.

Simone Weyand1, Georgia Kefala, Manfred S Weiss.   

Abstract

N-Succinyldiaminopimelate aminotransferase from Mycobacterium tuberculosis (DAP-AT; DapC; Rv0858c) has been cloned, heterologously expressed in Escherichia coli, purified using standard chromatographic techniques and crystallized in two related crystal forms. Preliminary diffraction data analysis suggests the presence of a monomer in the asymmetric unit of the tetragonal crystal form and a dimer in the asymmetric unit of the orthorhombic crystal form.

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Year:  2006        PMID： 16880560      PMCID： PMC2242922          DOI： 10.1107/S1744309106026753

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  10 in total

Review 1.  The manifold of vitamin B6 dependent enzymes.

Authors:  G Schneider; H Käck; Y Lindqvist
Journal:  Structure       Date:  2000-01-15       Impact factor: 5.006

2.  SFCHECK: a unified set of procedures for evaluating the quality of macromolecular structure-factor data and their agreement with the atomic model.

Authors:  A A Vaguine; J Richelle; S J Wodak
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  1999-01-01

Review 3.  Inhibitors of lysine biosynthesis as antibacterial agents.

Authors:  Craig A Hutton; Timothy J Southwood; Jennifer J Turner
Journal:  Mini Rev Med Chem       Date:  2003-03       Impact factor: 3.862

4.  N-Succinyl-L-diaminopimelic-glutamic transaminase.

Authors:  B PETERKOFSKY; C GILVARG
Journal:  J Biol Chem       Date:  1961-05       Impact factor: 5.157

5.  The CCP4 suite: programs for protein crystallography.

Authors: 
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  1994-09-01

6.  Solvent content of protein crystals.

Authors:  B W Matthews
Journal:  J Mol Biol       Date:  1968-04-28       Impact factor: 5.469

7.  Characterization of a bordetella pertussis diaminopimelate (DAP) biosynthesis locus identifies dapC, a novel gene coding for an N-succinyl-L,L-DAP aminotransferase.

Authors:  T M Fuchs; B Schneider; K Krumbach; L Eggeling; R Gross
Journal:  J Bacteriol       Date:  2000-07       Impact factor: 3.490

8.  Peptide inhibitors of N-succinyl diaminopimelic acid aminotransferase (DAP-AT): a novel class of antimicrobial compounds.

Authors:  R J Cox; J A Schouten; R A Stentiford; K J Wareing
Journal:  Bioorg Med Chem Lett       Date:  1998-04-21       Impact factor: 2.823

9.  Identification and characterization of the last two unknown genes, dapC and dapF, in the succinylase branch of the L-lysine biosynthesis of Corynebacterium glutamicum.

Authors:  Michael Hartmann; Andreas Tauch; Lothar Eggeling; Brigitte Bathe; Bettina Möckel; Alfred Pühler; Jörn Kalinowski
Journal:  J Biotechnol       Date:  2003-09-04       Impact factor: 3.307

10.  Influence of the codon following the AUG initiation codon on the expression of a modified lacZ gene in Escherichia coli.

Authors:  A C Looman; J Bodlaender; L J Comstock; D Eaton; P Jhurani; H A de Boer; P H van Knippenberg
Journal:  EMBO J       Date:  1987-08       Impact factor: 11.598
  10 in total
  5 in total

1.  The three-dimensional structure of diaminopimelate decarboxylase from Mycobacterium tuberculosis reveals a tetrameric enzyme organisation.

Authors:  Simone Weyand; Georgia Kefala; Dmitri I Svergun; Manfred S Weiss
Journal:  J Struct Funct Genomics       Date:  2009-06-19

2.  Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of tetrahydrodipicolinate-N-succinyltransferase (Rv1201c) from Mycobacterium tuberculosis.

Authors:  Linda Schuldt; Simone Weyand; Georgia Kefala; Manfred S Weiss
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2008-08-29

3.  Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of the regulatory domain of aspartokinase (Rv3709c) from Mycobacterium tuberculosis.

Authors:  Linda Schuldt; Ruth Suchowersky; Katharina Veith; Jochen Mueller-Dieckmann; Manfred S Weiss
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2011-02-25

4.  Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of succinyl-diaminopimelate desuccinylase (Rv1202, DapE) from Mycobacterium tuberculosis.

Authors:  Linda Reinhard; Jochen Mueller-Dieckmann; Manfred S Weiss
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2012-08-31

5.  Nucleotide-oligomerizing domain-1 (NOD1) receptor activation induces pro-inflammatory responses and autophagy in human alveolar macrophages.

Authors:  Esmeralda Juárez; Claudia Carranza; Fernando Hernández-Sánchez; Elva Loyola; Dante Escobedo; Juan Carlos León-Contreras; Rogelio Hernández-Pando; Martha Torres; Eduardo Sada
Journal:  BMC Pulm Med       Date:  2014-09-25       Impact factor: 3.317
  5 in total

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