BACKGROUND: Evidence suggests that the growth arrest and DNA damage-inducible gene 45 (Gadd45) is an effective indicator of poor prognosis or malignant potential in solid tumors. The purpose of this study was to determine the gene expression patterns and clinical relevance of Gadd45 in tumor specimens of non-small cell lung cancer (NSCLC) patients. PATIENTS AND METHODS: Using a quantitative real-time RT-PCR method, the mRNA expression of Gadd45 was analyzed in tumor and paired normal-appearing tissues of 66 patients with NSCLC. The gene expression data for each patient were matched to the clinicopathological parameters. RESULTS: Gadd45 mRNA expression was detectable in all (100%) specimens analyzed. The overall median mRNA expression level of Gadd45 was approximately 10-fold lower in tumor tissues than in matching normal lung tissues (p < 0.001). High intratumoral Gadd45 expression was significantly associated with a poorer histological grading (p = 0.041). CONCLUSION: The significant decrease in Gadd45 expression in tumors compared with normal tissue and its association with histological grading suggest a role for Gadd45 in the differentiation pathway of NSCLC.
BACKGROUND: Evidence suggests that the growth arrest and DNA damage-inducible gene 45 (Gadd45) is an effective indicator of poor prognosis or malignant potential in solid tumors. The purpose of this study was to determine the gene expression patterns and clinical relevance of Gadd45 in tumor specimens of non-small cell lung cancer (NSCLC) patients. PATIENTS AND METHODS: Using a quantitative real-time RT-PCR method, the mRNA expression of Gadd45 was analyzed in tumor and paired normal-appearing tissues of 66 patients with NSCLC. The gene expression data for each patient were matched to the clinicopathological parameters. RESULTS:Gadd45 mRNA expression was detectable in all (100%) specimens analyzed. The overall median mRNA expression level of Gadd45 was approximately 10-fold lower in tumor tissues than in matching normal lung tissues (p < 0.001). High intratumoral Gadd45 expression was significantly associated with a poorer histological grading (p = 0.041). CONCLUSION: The significant decrease in Gadd45 expression in tumors compared with normal tissue and its association with histological grading suggest a role for Gadd45 in the differentiation pathway of NSCLC.
Authors: Jan Brabender; Daniel Vallböhmer; Peter Grimminger; Andreas C Hoffmann; Frederike Ling; Georg Lurje; Elfriede Bollschweiler; Paul M Schneider; Arnulf H Hölscher; Ralf Metzger Journal: J Gastrointest Surg Date: 2008-09-03 Impact factor: 3.452
Authors: Maria Nikodemova; Jeremiah Yee; Patrick R Carney; Christopher A Bradfield; Kristen Mc Malecki Journal: Environ Int Date: 2018-02-16 Impact factor: 9.621
Authors: Cunzhong Yuan; Xiaoyan Liu; Xiaolin Liu; Ning Yang; Zhenping Liu; Shi Yan; Keng Shen; Beihua Kong Journal: PLoS One Date: 2015-09-30 Impact factor: 3.240
Authors: M Niedergethmann; F Alves; J K Neff; B Heidrich; N Aramin; L Li; C Pilarsky; R Grützmann; H Allgayer; S Post; N Gretz Journal: Br J Cancer Date: 2007-10-16 Impact factor: 7.640