Literature DB >> 16672413

Rapid detection of Brucella spp. in blood cultures by fluorescence in situ hybridization.

Nele Wellinghausen1, Karsten Nöckler, Anja Sigge, Melanie Bartel, Andreas Essig, Sven Poppert.   

Abstract

Brucellosis is a severe systemic disease in humans. We describe a new 16S rRNA-based fluorescence in situ hybridization assay that facilitates rapid and specific detection of all human pathogenic species of Brucella and that can be applied directly to positive blood cultures.

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Year:  2006        PMID： 16672413      PMCID： PMC1479171          DOI： 10.1128/JCM.44.5.1828-1830.2006

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  14 in total

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Authors:  P Yagupsky
Journal:  J Clin Microbiol       Date:  1999-11       Impact factor: 5.948

2.  Fluorescent In situ hybridization allows rapid identification of microorganisms in blood cultures.

Authors:  V A Kempf; K Trebesius; I B Autenrieth
Journal:  J Clin Microbiol       Date:  2000-02       Impact factor: 5.948

3.  Use of 16S rRNA gene sequencing for rapid confirmatory identification of Brucella isolates.

Authors:  Jay E Gee; Barun K De; Paul N Levett; Anne M Whitney; Ryan T Novak; Tanja Popovic
Journal:  J Clin Microbiol       Date:  2004-08       Impact factor: 5.948

4.  Real-time multiplex PCR assay for detection of Brucella spp., B. abortus, and B. melitensis.

Authors:  William S Probert; Kimmi N Schrader; Nhi Y Khuong; Susan L Bystrom; Margot H Graves
Journal:  J Clin Microbiol       Date:  2004-03       Impact factor: 5.948

5.  Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations.

Authors:  R I Amann; B J Binder; R J Olson; S W Chisholm; R Devereux; D A Stahl
Journal:  Appl Environ Microbiol       Date:  1990-06       Impact factor: 4.792

6.  Rapid diagnosis of bacterial meningitis by real-time PCR and fluorescence in situ hybridization.

Authors:  Sven Poppert; Andreas Essig; Barbara Stoehr; Adelinde Steingruber; Beate Wirths; Stefan Juretschko; Udo Reischl; Nele Wellinghausen
Journal:  J Clin Microbiol       Date:  2005-07       Impact factor: 5.948

7.  Brucellosis from sniffing bacteriological cultures.

Authors:  M Grammont-Cupillard; L Berthet-Badetti; P Dellamonica
Journal:  Lancet       Date:  1996 Dec 21-28       Impact factor: 79.321

8.  Fluorescent-oligonucleotide probing of whole cells for determinative, phylogenetic, and environmental studies in microbiology.

Authors:  R I Amann; L Krumholz; D A Stahl
Journal:  J Bacteriol       Date:  1990-02       Impact factor: 3.490

Review 9.  The detection of Brucella spp. using PCR-ELISA and real-time PCR assays.

Authors:  Sascha Al Dahouk; Herbert Tomaso; Karsten Nöckler; Heinrich Neubauer
Journal:  Clin Lab       Date:  2004       Impact factor: 1.138

10.  Biochemical mis-identification of Brucella melitensis and subsequent laboratory-acquired infections.

Authors:  B I Batchelor; R J Brindle; G F Gilks; J B Selkon
Journal:  J Hosp Infect       Date:  1992-10       Impact factor: 3.926
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6.  Rapid differentiation of Francisella species and subspecies by fluorescent in situ hybridization targeting the 23S rRNA.

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8.  Beacon-based (bbFISH®) technology for rapid pathogens identification in blood cultures.

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9.  NOTIFy (non-toxic lyophilized field)-FISH for the identification of biological agents by Fluorescence in situ Hybridization.

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Review 10.  Microbiological Laboratory Diagnosis of Human Brucellosis: An Overview.

Authors:  Giovanni Di Bonaventura; Silvia Angeletti; Andrea Ianni; Tommasangelo Petitti; Giovanni Gherardi
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  10 in total

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