Literature DB >> 15004098

Real-time multiplex PCR assay for detection of Brucella spp., B. abortus, and B. melitensis.

William S Probert1, Kimmi N Schrader, Nhi Y Khuong, Susan L Bystrom, Margot H Graves.   

Abstract

The identification of Brucella can be a time-consuming and labor-intensive process that places personnel at risk for laboratory-acquired infection. Here, we describe a real-time PCR assay for confirmation of presumptive Brucella isolates. The assay was designed in a multiplex format that will allow the rapid identification of Brucella spp., B. abortus, and B. melitensis in a single test.

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Year:  2004        PMID： 15004098      PMCID： PMC356861          DOI： 10.1128/JCM.42.3.1290-1293.2004

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  14 in total

1.  Contamination and sensitivity issues with a real-time universal 16S rRNA PCR.

Authors:  C E Corless; M Guiver; R Borrow; V Edwards-Jones; E B Kaczmarski; A J Fox
Journal:  J Clin Microbiol       Date:  2000-05       Impact factor: 5.948

2.  A twenty-five year review of laboratory-acquired human infections at the National Animal Disease Center.

Authors:  C D Miller; J R Songer; J F Sullivan
Journal:  Am Ind Hyg Assoc J       Date:  1987-03

3.  Comparison of three different PCR methods for detection of Brucella spp in human blood samples.

Authors:  E Navarro; J Escribano; J Fernández; J Solera
Journal:  FEMS Immunol Med Microbiol       Date:  2002-10-11

4.  Nucleotide sequence and expression of the gene encoding the major 25-kilodalton outer membrane protein of Brucella ovis: Evidence for antigenic shift, compared with other Brucella species, due to a deletion in the gene.

Authors:  A Cloeckaert; J M Verger; M Grayon; M S Zygmunt; O Grépinet
Journal:  Infect Immun       Date:  1996-06       Impact factor: 3.441

5.  Serum is the preferred clinical specimen for diagnosis of human brucellosis by PCR.

Authors:  L Zerva; K Bourantas; S Mitka; A Kansouzidou; N J Legakis
Journal:  J Clin Microbiol       Date:  2001-04       Impact factor: 5.948

6.  Human neurobrucellosis with intracerebral granuloma caused by a marine mammal Brucella spp.

Authors:  Annette H Sohn; Will S Probert; Carol A Glaser; Nalin Gupta; Andrew W Bollen; Jane D Wong; Elizabeth M Grace; William C McDonald
Journal:  Emerg Infect Dis       Date:  2003-04       Impact factor: 6.883

7.  Real-time detection of Brucella abortus, Brucella melitensis and Brucella suis.

Authors:  R Redkar; S Rose; B Bricker; V DelVecchio
Journal:  Mol Cell Probes       Date:  2001-02       Impact factor: 2.365

8.  Development and evaluation of a PCR-enzyme-linked immunosorbent assay for diagnosis of human brucellosis.

Authors:  Pilar Morata; María I Queipo-Ortuño; Jose M Reguera; Miguel A García-Ordoñez; Ana Cárdenas; Juan D Colmenero
Journal:  J Clin Microbiol       Date:  2003-01       Impact factor: 5.948

9.  Detection of Brucella melitensis and Brucella abortus by DNA amplification.

Authors:  G G Baily; J B Krahn; B S Drasar; N G Stoker
Journal:  J Trop Med Hyg       Date:  1992-08

10.  Time-space clustering of human brucellosis, California, 1973-1992.

Authors:  Geoffrey T Fosgate; Tim E Carpenter; Bruno B Chomel; James T Case; Emilio E DeBess; Kevin F Reilly
Journal:  Emerg Infect Dis       Date:  2002-07       Impact factor: 6.883
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  69 in total

1.  Use of 16S rRNA gene sequencing for rapid confirmatory identification of Brucella isolates.

Authors:  Jay E Gee; Barun K De; Paul N Levett; Anne M Whitney; Ryan T Novak; Tanja Popovic
Journal:  J Clin Microbiol       Date:  2004-08       Impact factor: 5.948

2.  Comparative diagnostic evaluation of OMP31 gene based TaqMan® real-time PCR assay with visual LAMP assay and indirect ELISA for caprine brucellosis.

Authors:  Suman Saini; V K Gupta; K Gururaj; D D Singh; R V S Pawaiya; N K Gangwar; A K Mishra; Deepak Dwivedi; Dimple Andani; Ashok Kumar; T K Goswami
Journal:  Trop Anim Health Prod       Date:  2017-06-21       Impact factor: 1.559

3.  Real-time PCR assays of single-nucleotide polymorphisms defining the major Brucella clades.

Authors:  Jeffrey T Foster; Richard T Okinaka; Rita Svensson; Kathryn Shaw; Barun K De; Richard A Robison; William S Probert; Leo J Kenefic; William D Brown; Paul Keim
Journal:  J Clin Microbiol       Date:  2007-11-21       Impact factor: 5.948

4.  Multiplexed identification of blood-borne bacterial pathogens by use of a novel 16S rRNA gene PCR-ligase detection reaction-capillary electrophoresis assay.

Authors:  Maneesh R Pingle; Kathleen Granger; Philip Feinberg; Rebecca Shatsky; Bram Sterling; Mark Rundell; Eric Spitzer; Davise Larone; Linnie Golightly; Francis Barany
Journal:  J Clin Microbiol       Date:  2007-04-11       Impact factor: 5.948

5.  Optimization and validation of a real-time polymerase chain reaction protocol for the diagnosis of human brucellosis.

Authors:  Hasan Zeybek; Ziya Cibali Acikgoz; Tuba Dal; Rıza Durmaz
Journal:  Folia Microbiol (Praha)       Date:  2019-07-31       Impact factor: 2.099

6.  Real-time PCR for detection of Brucella spp. DNA in human serum samples.

Authors:  C Debeaumont; P A Falconnet; M Maurin
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2005-12       Impact factor: 3.267

7.  Retrospective study of etiologic agents associated with nonsuppurative meningoencephalitis in stranded cetaceans in the canary islands.

Authors:  Eva Sierra; Susan Sánchez; Jeremiah T Saliki; Uriel Blas-Machado; Manuel Arbelo; Daniele Zucca; Antonio Fernández
Journal:  J Clin Microbiol       Date:  2014-04-23       Impact factor: 5.948

Review 8.  Review of brucellosis in Nepal.

Authors:  Krishna Prasad Acharya; Krishna Kaphle; Kshitiz Shrestha; Bruno Garin Bastuji; Henk L Smits
Journal:  Int J Vet Sci Med       Date:  2016-12-16

9.  Reverse line blot macroarray for simultaneous detection and characterization of four biological warfare agents.

Authors:  Kulanthaivel Thavachelvam; Urmil Tuteja; Kumari Sarika; Suryanarayana Nagendra; Subodh Kumar
Journal:  Indian J Microbiol       Date:  2012-11-06       Impact factor: 2.461

10.  IS711-based real-time PCR assay as a tool for detection of Brucella spp. in wild boars and comparison with bacterial isolation and serology.

Authors:  Vladimira Hinić; Isabelle Brodard; Andreas Thomann; Milena Holub; Raymond Miserez; Carlos Abril
Journal:  BMC Vet Res       Date:  2009-07-14       Impact factor: 2.741
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