Literature DB >> 16637621

Hydride transfer versus hydrogen radical transfer in thymidylate synthase.

Baoyu Hong1, Majd Haddad, Frank Maley, Jan H Jensen, Amnon Kohen.   

Abstract

The nature of a H-transfer in the thymidylate synthase catalyzed reaction was investigated by comparison of the wild-type enzyme with the W80M mutant. The nature of the H-transfer was not affected, as indicated by intrinsic isotope effects and their temperature dependence. These findings support a single-step hydride transfer instead of a two-step radical transfer.

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Year:  2006        PMID： 16637621      PMCID： PMC3253763          DOI： 10.1021/ja060196o

Source DB:  PubMed          Journal:  J Am Chem Soc        ISSN： 0002-7863            Impact factor:   15.419


  15 in total

1.  The Cationminus signpi Interaction.

Authors:  Jennifer C. Ma; Dennis A. Dougherty
Journal:  Chem Rev       Date:  1997-08-05       Impact factor: 60.622

2.  Temperature-dependent isotope effects in soybean lipoxygenase-1: correlating hydrogen tunneling with protein dynamics.

Authors:  Michael J Knapp; Keith Rickert; Judith P Klinman
Journal:  J Am Chem Soc       Date:  2002-04-17       Impact factor: 15.419

Review 3.  Conformational dynamics along an enzymatic reaction pathway: thymidylate synthase, "the movie".

Authors:  Robert M Stroud; Janet S Finer-Moore
Journal:  Biochemistry       Date:  2003-01-21       Impact factor: 3.162

Review 4.  Lessons and conclusions from dissecting the mechanism of a bisubstrate enzyme: thymidylate synthase mutagenesis, function, and structure.

Authors:  Janet S Finer-Moore; Daniel V Santi; Robert M Stroud
Journal:  Biochemistry       Date:  2003-01-21       Impact factor: 3.162

5.  The role of enzyme dynamics and tunnelling in catalysing hydride transfer: studies of distal mutants of dihydrofolate reductase.

Authors:  Lin Wang; Nina M Goodey; Stephen J Benkovic; Amnon Kohen
Journal:  Philos Trans R Soc Lond B Biol Sci       Date:  2006-08-29       Impact factor: 6.237

6.  Effects of a distal mutation on active site chemistry.

Authors:  Lin Wang; Scott Tharp; Tzvia Selzer; Stephen J Benkovic; Amnon Kohen
Journal:  Biochemistry       Date:  2006-02-07       Impact factor: 3.162

7.  Nature of hydrogen transfer in soybean lipoxygenase 1: separation of primary and secondary isotope effects.

Authors:  K W Rickert; J P Klinman
Journal:  Biochemistry       Date:  1999-09-21       Impact factor: 3.162

8.  Enzyme dynamics and hydrogen tunnelling in a thermophilic alcohol dehydrogenase.

Authors:  A Kohen; R Cannio; S Bartolucci; J P Klinman
Journal:  Nature       Date:  1999-06-03       Impact factor: 49.962

9.  Microscale synthesis of isotopically labeled R-[6-xH]N5,N10-methylene-5,6,7,8-tetrahydrofolate as a cofactor for thymidylate synthase.

Authors:  Nitish Agrawal; Cornelia Mihai; Amnon Kohen
Journal:  Anal Biochem       Date:  2004-05-01       Impact factor: 3.365

Review 10.  Hydrogen tunneling and protein motion in enzyme reactions.

Authors:  Sharon Hammes-Schiffer
Journal:  Acc Chem Res       Date:  2006-02       Impact factor: 22.384
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  12 in total

1.  Widespread Perturbation of Function, Structure, and Dynamics by a Conservative Single-Atom Substitution in Thymidylate Synthase.

Authors:  Paul J Sapienza; Andrew L Lee
Journal:  Biochemistry       Date:  2016-09-30       Impact factor: 3.162

2.  Thymidylate synthase catalyzed H-transfers: two chapters in one tale.

Authors:  Zhen Wang; Amnon Kohen
Journal:  J Am Chem Soc       Date:  2010-07-21       Impact factor: 15.419

3.  Preserved hydride transfer mechanism in evolutionarily divergent thymidylate synthases.

Authors:  Thelma Abeysinghe; Baoyu Hong; Zhen Wang; Amnon Kohen
Journal:  Curr Top Biochem Res       Date:  2016

4.  Temperature dependence of the kinetic isotope effects in thymidylate synthase. A theoretical study.

Authors:  Natalia Kanaan; Silvia Ferrer; Sergio Martí; Mireia Garcia-Viloca; Amnon Kohen; Vicent Moliner
Journal:  J Am Chem Soc       Date:  2011-04-08       Impact factor: 15.419

5.  A remote mutation affects the hydride transfer by disrupting concerted protein motions in thymidylate synthase.

Authors:  Zhen Wang; Thelma Abeysinghe; Janet S Finer-Moore; Robert M Stroud; Amnon Kohen
Journal:  J Am Chem Soc       Date:  2012-10-15       Impact factor: 15.419

6.  Role of Y94 in proton and hydride transfers catalyzed by thymidylate synthase.

Authors:  Baoyu Hong; Frank Maley; Amnon Kohen
Journal:  Biochemistry       Date:  2007-11-14       Impact factor: 3.162

7.  RNA-assisted catalysis in a protein enzyme: The 2'-hydroxyl of tRNA(Thr) A76 promotes aminoacylation by threonyl-tRNA synthetase.

Authors:  Anand Minajigi; Christopher S Francklyn
Journal:  Proc Natl Acad Sci U S A       Date:  2008-11-07       Impact factor: 11.205

Review 8.  Relationship of femtosecond-picosecond dynamics to enzyme-catalyzed H-transfer.

Authors:  Christopher M Cheatum; Amnon Kohen
Journal:  Top Curr Chem       Date:  2013

9.  Activation of Two Sequential H-transfers in the Thymidylate Synthase Catalyzed Reaction.

Authors:  Zahidul Islam; Timothy S Strutzenberg; Ananda K Ghosh; Amnon Kohen
Journal:  ACS Catal       Date:  2015-09-02       Impact factor: 13.084

10.  Role of an invariant lysine residue in folate binding on Escherichia coli thymidylate synthase: calorimetric and crystallographic analysis of the K48Q mutant.

Authors:  Aldo A Arvizu-Flores; Rocio Sugich-Miranda; Rodrigo Arreola; Karina D Garcia-Orozco; Enrique F Velazquez-Contreras; William R Montfort; Frank Maley; Rogerio R Sotelo-Mundo
Journal:  Int J Biochem Cell Biol       Date:  2008-03-06       Impact factor: 5.085
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