Literature DB >> 16628205

A highly flexible tRNA acylation method for non-natural polypeptide synthesis.

Hiroshi Murakami1, Atsushi Ohta, Hiroshi Ashigai, Hiroaki Suga.   

Abstract

Here we describe a de novo tRNA acylation system, the flexizyme (Fx) system, for the preparation of acyl tRNAs with nearly unlimited selection of amino and hydroxy acids and tRNAs. The combination of the Fx system with an appropriate cell-free translation system allows us to readily perform mRNA-encoded synthesis of proteins and short polypeptides involving multiple non-natural amino acids.

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Year:  2006        PMID： 16628205     DOI： 10.1038/nmeth877

Source DB:  PubMed          Journal:  Nat Methods        ISSN： 1548-7091            Impact factor:   28.547


  108 in total

1.  Flexizymes for genetic code reprogramming.

Authors:  Yuki Goto; Takayuki Katoh; Hiroaki Suga
Journal:  Nat Protoc       Date:  2011-05-12       Impact factor: 13.491

2.  The RNA origin of transfer RNA aminoacylation and beyond.

Authors:  Hiroaki Suga; Gosuke Hayashi; Naohiro Terasaka
Journal:  Philos Trans R Soc Lond B Biol Sci       Date:  2011-10-27       Impact factor: 6.237

3.  tRNAArg-Derived Fragments Can Serve as Arginine Donors for Protein Arginylation.

Authors:  Irem Avcilar-Kucukgoze; Howard Gamper; Christine Polte; Zoya Ignatova; Ralph Kraetzner; Michael Shtutman; Ya-Ming Hou; Dawei W Dong; Anna Kashina
Journal:  Cell Chem Biol       Date:  2020-06-16       Impact factor: 8.116

4.  A recurrent loss-of-function alanyl-tRNA synthetase (AARS) mutation in patients with Charcot-Marie-Tooth disease type 2N (CMT2N).

Authors:  Heather M McLaughlin; Reiko Sakaguchi; William Giblin; Thomas E Wilson; Leslie Biesecker; James R Lupski; Kevin Talbot; Jeffery M Vance; Stephan Züchner; Yi-Chung Lee; Marina Kennerson; Ya-Ming Hou; Garth Nicholson; Anthony Antonellis
Journal:  Hum Mutat       Date:  2011-11-09       Impact factor: 4.878

Review 5.  Foldamers as versatile frameworks for the design and evolution of function.

Authors:  Catherine M Goodman; Sungwook Choi; Scott Shandler; William F DeGrado
Journal:  Nat Chem Biol       Date:  2007-05       Impact factor: 15.040

6.  Dual Genetic Encoding of Acetyl-lysine and Non-deacetylatable Thioacetyl-lysine Mediated by Flexizyme.

Authors:  Hai Xiong; Noah M Reynolds; Chenguang Fan; Markus Englert; Denton Hoyer; Scott J Miller; Dieter Söll
Journal:  Angew Chem Int Ed Engl       Date:  2016-02-23       Impact factor: 15.336

7.  Quality control by trans-editing factor prevents global mistranslation of non-protein amino acid α-aminobutyrate.

Authors:  Jo Marie Bacusmo; Alexandra B Kuzmishin; William A Cantara; Yuki Goto; Hiroaki Suga; Karin Musier-Forsyth
Journal:  RNA Biol       Date:  2017-11-03       Impact factor: 4.652

8.  Homologous trans-editing factors with broad tRNA specificity prevent mistranslation caused by serine/threonine misactivation.

Authors:  Ziwei Liu; Oscar Vargas-Rodriguez; Yuki Goto; Eva Maria Novoa; Lluís Ribas de Pouplana; Hiroaki Suga; Karin Musier-Forsyth
Journal:  Proc Natl Acad Sci U S A       Date:  2015-04-27       Impact factor: 11.205

9.  Changeability of individual domains of an aminoacyl-tRNA in polymerization by the ribosome.

Authors:  Rong Gao; Anthony C Forster
Journal:  FEBS Lett       Date:  2010-01-04       Impact factor: 4.124

10.  Unnatural amino acids: better than the real things?

Authors:  Ellen C Minnihan; Kenichi Yokoyama; Joanne Stubbe
Journal:  F1000 Biol Rep       Date:  2009-11-26
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