Literature DB >> 6255421

DNA gyrase subunit stoichiometry and the covalent attachment of subunit A to DNA during DNA cleavage.

A Sugino, N P Higgins, N R Cozzarelli.   

Abstract

Escherichia coli DNA gyrase contains a 1:1 ratio of protomers coded by the genes gyrA and gyrB. This along with previous results shows that the enzyme has two copies of each protomer and thus a molecular weight of 400,000. Abortion of the gyrase reaction results in double-strand breakage of the DNA and covalent attachment of both gyrA protomers to the 5'-cut ends. We conclude that the gyrA protomer contains a critical part of the active site for the concerted breakage and reunion reaction of gyrase, the topoisomerase activity of the enzyme.

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Year:  1980        PMID： 6255421      PMCID： PMC324200          DOI： 10.1093/nar/8.17.3865

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  24 in total

1.  Identification of a protein linked to the ends of adenovirus DNA.

Authors:  D M Rekosh; W C Russell; A J Bellet; A J Robinson
Journal:  Cell       Date:  1977-06       Impact factor: 41.582

2.  Strand breakage by the DNA untwisting enzyme results in covalent attachment of the enzyme to DNA.

Authors:  J J Champoux
Journal:  Proc Natl Acad Sci U S A       Date:  1977-09       Impact factor: 11.205

3.  Mechanism of action of nalidixic acid: purification of Escherichia coli nalA gene product and its relationship to DNA gyrase and a novel nicking-closing enzyme.

Authors:  A Sugino; C L Peebles; K N Kreuzer; N R Cozzarelli
Journal:  Proc Natl Acad Sci U S A       Date:  1977-11       Impact factor: 11.205

4.  A study of adenosine 3'-5' cyclic monophosphate binding sites of human erythrocyte membranes using 8-azidoadenosine 3'-5' cyclic monophosphate, a photoaffinity probe.

Authors:  J R Owens; B E Haley
Journal:  J Supramol Struct       Date:  1976

5.  DNA gyrase: an enzyme that introduces superhelical turns into DNA.

Authors:  M Gellert; K Mizuuchi; M H O'Dea; H A Nash
Journal:  Proc Natl Acad Sci U S A       Date:  1976-11       Impact factor: 11.205

6.  Interaction between DNA and an Escherichia coli protein omega.

Authors:  J C Wang
Journal:  J Mol Biol       Date:  1971-02-14       Impact factor: 5.469

7.  Fate of parental phi X174-DNA upon infection of starved thymine-requiring host cells.

Authors:  B Francke; D S Ray
Journal:  Virology       Date:  1971-04       Impact factor: 3.616

8.  Replication of small plasmids in extracts of Escherichia coli.

Authors:  W L Staudenbauer
Journal:  Mol Gen Genet       Date:  1976-06-15

9.  Processing of adenovirus 2-induced proteins.

Authors:  C W Anderson; P R Baum; R F Gesteland
Journal:  J Virol       Date:  1973-08       Impact factor: 5.103

10.  Nalidixic acid resistance: a second genetic character involved in DNA gyrase activity.

Authors:  M Gellert; K Mizuuchi; M H O'Dea; T Itoh; J I Tomizawa
Journal:  Proc Natl Acad Sci U S A       Date:  1977-11       Impact factor: 11.205
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  27 in total

1.  Spontaneous quinolone resistance in Serratia marcescens due to a mutation in gyrA.

Authors:  B L Masecar; N J Robillard
Journal:  Antimicrob Agents Chemother       Date:  1991-05       Impact factor: 5.191

2.  Multiple consecutive lavage samplings reveal greater burden of disease and provide direct access to the nontypeable Haemophilus influenzae biofilm in experimental otitis media.

Authors:  Magali Leroy; Howard Cabral; Marisol Figueira; Valérie Bouchet; Heather Huot; Sanjay Ram; Stephen I Pelton; Richard Goldstein
Journal:  Infect Immun       Date:  2007-05-21       Impact factor: 3.441

Review 3.  In vitro assays used to measure the activity of topoisomerases.

Authors:  J F Barrett; J A Sutcliffe; T D Gootz
Journal:  Antimicrob Agents Chemother       Date:  1990-01       Impact factor: 5.191

4.  NBTI 5463 is a novel bacterial type II topoisomerase inhibitor with activity against gram-negative bacteria and in vivo efficacy.

Authors:  Thomas J Dougherty; Asha Nayar; Joseph V Newman; Sussie Hopkins; Gregory G Stone; Michele Johnstone; Adam B Shapiro; Mark Cronin; Folkert Reck; David E Ehmann
Journal:  Antimicrob Agents Chemother       Date:  2014-02-24       Impact factor: 5.191

5.  The acidic C-terminal tail of the GyrA subunit moderates the DNA supercoiling activity of Bacillus subtilis gyrase.

Authors:  Martin A Lanz; Mohamad Farhat; Dagmar Klostermeier
Journal:  J Biol Chem       Date:  2014-02-20       Impact factor: 5.157

6.  The 52-protein subunit of T4 DNA topoisomerase is homologous to the gyrA-protein of gyrase.

Authors:  W M Huang
Journal:  Nucleic Acids Res       Date:  1986-09-25       Impact factor: 16.971

7.  Purification and properties of DNA gyrase from Vibrio cholerae.

Authors:  G Mukhopadhyay; A Ghosh
Journal:  Biochem J       Date:  1991-12-15       Impact factor: 3.857

8.  Mapping the active-site tyrosine of vaccinia virus DNA topoisomerase I.

Authors:  S Shuman; E M Kane; S G Morham
Journal:  Proc Natl Acad Sci U S A       Date:  1989-12       Impact factor: 11.205

9.  Covalent protein-DNA complexes at the 5' strand termini of meiosis-specific double-strand breaks in yeast.

Authors:  S Keeney; N Kleckner
Journal:  Proc Natl Acad Sci U S A       Date:  1995-11-21       Impact factor: 11.205

10.  Characterization of functionally important sites in the bacteriophage Mu transposase protein.

Authors:  P I Ulycznyj; F Forghani; M S DuBow
Journal:  Mol Gen Genet       Date:  1994-02
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