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Literature DB >> 16554830

Efficient incorporation of unnatural amino acids into proteins in Escherichia coli.

Abstract

We have developed a single-plasmid system for the efficient bacterial expression of mutant proteins containing unnatural amino acids at specific sites designated by amber nonsense codons. In this system, multiple copies of a gene encoding an amber suppressor tRNA derived from a Methanocaldococcus jannaschii tyrosyl-tRNA (MjtRNATyrCUA) are expressed under control of the proK promoter and terminator, and a gene encoding the desired mutant M. jannaschii tyrosyl-tRNA synthetase (MjTyrRS) is expressed under control of a mutant glnS (glnS') promoter.

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Year: 2006 PMID： 16554830 DOI： 10.1038/nmeth864

Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547

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Cited

107 in total

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2. Mapping precursor-binding site on TatC subunit of twin arginine-specific protein translocase by site-specific photo cross-linking.

Authors: Stefan Zoufaly; Julia Fröbel; Patrick Rose; Tobias Flecken; Carlo Maurer; Michael Moser; Matthias Müller
Journal: J Biol Chem Date: 2012-02-23 Impact factor: 5.157

3. Experimental methods for scanning unnatural amino acid mutagenesis.

Authors: Jia Liu; T Ashton Cropp
Journal: Methods Mol Biol Date: 2012

4. Site-specific fluorescent labeling of poly-histidine sequences using a metal-chelating cysteine.

Authors: Beena Krishnan; Aneta Szymanska; Lila M Gierasch
Journal: Chem Biol Drug Des Date: 2007-01 Impact factor: 2.817

5. A facile system for encoding unnatural amino acids in mammalian cells.

Authors: Peng R Chen; Dan Groff; Jiantao Guo; Weijia Ou; Susan Cellitti; Bernhard H Geierstanger; Peter G Schultz
Journal: Angew Chem Int Ed Engl Date: 2009 Impact factor: 15.336

6. Model of mouth-to-mouth transfer of bacterial lipoproteins through inner membrane LolC, periplasmic LolA, and outer membrane LolB.

Authors: Suguru Okuda; Hajime Tokuda
Journal: Proc Natl Acad Sci U S A Date: 2009-03-23 Impact factor: 11.205

Efficient incorporation of unnatural amino acids into proteins in Escherichia coli.

1. Early contacts between substrate proteins and TatA translocase component in twin-arginine translocation.

2. Mapping precursor-binding site on TatC subunit of twin arginine-specific protein translocase by site-specific photo cross-linking.

3. Experimental methods for scanning unnatural amino acid mutagenesis.

4. Site-specific fluorescent labeling of poly-histidine sequences using a metal-chelating cysteine.

5. A facile system for encoding unnatural amino acids in mammalian cells.

6. Model of mouth-to-mouth transfer of bacterial lipoproteins through inner membrane LolC, periplasmic LolA, and outer membrane LolB.

7. Protein evolution with an expanded genetic code.

Review 8. Incorporation of Non-Canonical Amino Acids.

9. Intersubunit cross-talk in pyridoxal 5'-phosphate synthase, coordinated by the C terminus of the synthase subunit.

10. RNA Recognition-like Motifs Activate a Mitogen-Activated Protein Kinase.