Literature DB >> 16537637

Recovery of a neurovirulent human coronavirus OC43 from an infectious cDNA clone.

Julien R St-Jean1, Marc Desforges, Fernando Almazán, Hélène Jacomy, Luis Enjuanes, Pierre J Talbot.   

Abstract

This study describes the assembly of a full-length cDNA clone of human coronavirus (HCoV)-OC43 in a bacterial artificial chromosome (BAC). The BAC containing the full-length infectious cDNA (pBAC-OC43(FL)) was assembled using a two-part strategy. The first step consisted in the introduction of each end of the viral genome into the BAC with accessory sequences allowing proper transcription. The second step consisted in the insertion of the whole HCoV-OC43 cDNA genome into the BAC. To produce recombinant viral particles, pBAC-OC43(FL) was transfected into BHK-21 cells. Recombinant virus displayed the same phenotypic properties as the wild-type virus, including infectious virus titers produced in cell culture and neurovirulence in mice.

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Year:  2006        PMID： 16537637      PMCID： PMC1440365          DOI： 10.1128/JVI.80.7.3670-3674.2006

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  21 in total

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4.  Role of nucleotides immediately flanking the transcription-regulating sequence core in coronavirus subgenomic mRNA synthesis.

Authors:  Isabel Sola; José L Moreno; Sonia Zúñiga; Sara Alonso; Luis Enjuanes
Journal:  J Virol       Date:  2005-02       Impact factor: 5.103

5.  Neuroinvasion by human respiratory coronaviruses.

Authors:  N Arbour; R Day; J Newcombe; P J Talbot
Journal:  J Virol       Date:  2000-10       Impact factor: 5.103

6.  Strategy for systematic assembly of large RNA and DNA genomes: transmissible gastroenteritis virus model.

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7.  The nucleoprotein is required for efficient coronavirus genome replication.

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8.  Recombinant mouse hepatitis virus strain A59 from cloned, full-length cDNA replicates to high titers in vitro and is fully pathogenic in vivo.

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Journal:  J Virol       Date:  2005-03       Impact factor: 5.103

9.  Human respiratory coronavirus OC43: genetic stability and neuroinvasion.

Authors:  Julien R St-Jean; Hélène Jacomy; Marc Desforges; Astrid Vabret; François Freymuth; Pierre J Talbot
Journal:  J Virol       Date:  2004-08       Impact factor: 5.103

Review 10.  Reverse genetics of the largest RNA viruses.

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  51 in total

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3.  Molecular characterization of feline infectious peritonitis virus strain DF-2 and studies of the role of ORF3abc in viral cell tropism.

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4.  Pivotal Role of Receptor-Interacting Protein Kinase 1 and Mixed Lineage Kinase Domain-Like in Neuronal Cell Death Induced by the Human Neuroinvasive Coronavirus OC43.

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Journal:  J Virol       Date:  2016-12-16       Impact factor: 5.103

5.  Genetic evolution of human coronavirus OC43 in neural cell culture.

Authors:  Julien R St-Jean; Marc Desforges; Pierre J Talbot
Journal:  Adv Exp Med Biol       Date:  2006       Impact factor: 2.622

6.  Construction of a severe acute respiratory syndrome coronavirus infectious cDNA clone and a replicon to study coronavirus RNA synthesis.

Authors:  Fernando Almazán; Marta L Dediego; Carmen Galán; David Escors; Enrique Alvarez; Javier Ortego; Isabel Sola; Sonia Zuñiga; Sara Alonso; Jose L Moreno; Aitor Nogales; Carmen Capiscol; Luis Enjuanes
Journal:  J Virol       Date:  2006-08-23       Impact factor: 5.103

7.  Isolation and characterization of current human coronavirus strains in primary human epithelial cell cultures reveal differences in target cell tropism.

Authors:  Ronald Dijkman; Maarten F Jebbink; Sylvie M Koekkoek; Martin Deijs; Hulda R Jónsdóttir; Richard Molenkamp; Margareta Ieven; Herman Goossens; Volker Thiel; Lia van der Hoek
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8.  Reverse genetic characterization of the natural genomic deletion in SARS-Coronavirus strain Frankfurt-1 open reading frame 7b reveals an attenuating function of the 7b protein in-vitro and in-vivo.

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9.  The acetyl-esterase activity of the hemagglutinin-esterase protein of human coronavirus OC43 strongly enhances the production of infectious virus.

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Journal:  J Virol       Date:  2013-01-02       Impact factor: 5.103

10.  The ns12.9 Accessory Protein of Human Coronavirus OC43 Is a Viroporin Involved in Virion Morphogenesis and Pathogenesis.

Authors:  Ronghua Zhang; Kai Wang; Xianqiang Ping; Wenjing Yu; Zhikang Qian; Sidong Xiong; Bing Sun
Journal:  J Virol       Date:  2015-09-02       Impact factor: 5.103
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