Literature DB >> 15507657

The nucleoprotein is required for efficient coronavirus genome replication.

Fernando Almazán1, Carmen Galán, Luis Enjuanes.   

Abstract

The construction of a set of transmissible gastroenteritis coronavirus (TGEV)-derived replicons as bacterial artificial chromosomes is reported. These replicons were generated by sequential deletion of nonessential genes for virus replication, using a modified TGEV full-length cDNA clone containing unique restriction sites between each pair of consecutive genes. Efficient activity of TGEV replicons was associated with the presence of the nucleoprotein provided either in cis or in trans. TGEV replicons were functional in several cell lines, including the human cell line 293T, in which no or very low cytopathic effect was observed, and expressed high amounts of heterologous protein.

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Year:  2004        PMID： 15507657      PMCID： PMC525053          DOI： 10.1128/JVI.78.22.12683-12688.2004

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  38 in total

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