| Literature DB >> 22438554 |
Ádám Bálint1, Attila Farsang, Zoltán Zádori, Ákos Hornyák, László Dencso, Fernando Almazán, Luis Enjuanes, Sándor Belák.
Abstract
The full-length genome of the highly lethal feline infectious peritonitis virus (FIPV) strain DF-2 was sequenced and cloned into a bacterial artificial chromosome (BAC) to study the role of ORF3abc in the FIPV-feline enteric coronavirus (FECV) transition. The reverse genetic system allowed the replacement of the truncated ORF3abc of the original FIPV DF-2 genome with the intact ORF3abc of the canine coronavirus (CCoV) reference strain Elmo/02. The in vitro replication kinetics of these two viruses was studied in CrFK and FCWF-4 cell lines, as well as in feline peripheral blood monocytes. Both viruses showed similar replication kinetics in established cell lines. However, the strain with a full-length ORF3 showed markedly lower replication of more than 2 log(10) titers in feline peripheral blood monocytes. Our results suggest that the truncated ORF3abc plays an important role in the efficient macrophage/monocyte tropism of type II FIPV.Entities:
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Year: 2012 PMID： 22438554 PMCID： PMC3372193 DOI： 10.1128/JVI.00189-12
Source DB: PubMed Journal: J Virol ISSN： 0022-538X Impact factor: 5.103