Literature DB >> 16511157

Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of LysA (Rv1293) from Mycobacterium tuberculosis.

Georgia Kefala1, L Jeanne Perry, Manfred S Weiss.   

Abstract

Diaminopimelate decarboxylase from Mycobacterium tuberculosis (LysA, DAPDC, Rv1293) has been cloned and heterologously expressed in Escherichia coli, purified using standard chromatographic techniques and crystallized. Preliminary diffraction data analysis suggests the presence of a homotetramer in the asymmetric unit.

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Year:  2005        PMID： 16511157      PMCID： PMC1952341          DOI： 10.1107/S1744309105022839

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  8 in total

1.  SFCHECK: a unified set of procedures for evaluating the quality of macromolecular structure-factor data and their agreement with the atomic model.

Authors:  A A Vaguine; J Richelle; S J Wodak
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  1999-01-01

2.  Cocrystal structures of diaminopimelate decarboxylase: mechanism, evolution, and inhibition of an antibiotic resistance accessory factor.

Authors:  Soumya S Ray; Jeffrey B Bonanno; K R Rajashankar; Mariana G Pinho; Guoshun He; Herminia De Lencastre; Alexander Tomasz; Stephen K Burley
Journal:  Structure       Date:  2002-11       Impact factor: 5.006

Review 3.  Inhibitors of lysine biosynthesis as antibacterial agents.

Authors:  Craig A Hutton; Timothy J Southwood; Jennifer J Turner
Journal:  Mini Rev Med Chem       Date:  2003-03       Impact factor: 3.862

4.  The CCP4 suite: programs for protein crystallography.

Authors: 
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  1994-09-01

5.  Processing of X-ray diffraction data collected in oscillation mode.

Authors:  Z Otwinowski; W Minor
Journal:  Methods Enzymol       Date:  1997       Impact factor: 1.600

6.  Crystallization of diaminopimelate decarboxylase from Escherichia coli, a stereospecific D-amino-acid decarboxylase.

Authors:  Cory Momany; Vladimir Levdikov; Lena Blagova; Kristine Crews
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2002-02-21

7.  Crystal structure of Mycobacterium tuberculosis diaminopimelate decarboxylase, an essential enzyme in bacterial lysine biosynthesis.

Authors:  Kuppan Gokulan; Bernhard Rupp; Martin S Pavelka; William R Jacobs; James C Sacchettini
Journal:  J Biol Chem       Date:  2003-03-10       Impact factor: 5.157

8.  Influence of the codon following the AUG initiation codon on the expression of a modified lacZ gene in Escherichia coli.

Authors:  A C Looman; J Bodlaender; L J Comstock; D Eaton; P Jhurani; H A de Boer; P H van Knippenberg
Journal:  EMBO J       Date:  1987-08       Impact factor: 11.598
  8 in total
  4 in total

1.  The three-dimensional structure of diaminopimelate decarboxylase from Mycobacterium tuberculosis reveals a tetrameric enzyme organisation.

Authors:  Simone Weyand; Georgia Kefala; Dmitri I Svergun; Manfred S Weiss
Journal:  J Struct Funct Genomics       Date:  2009-06-19

2.  Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of tetrahydrodipicolinate-N-succinyltransferase (Rv1201c) from Mycobacterium tuberculosis.

Authors:  Linda Schuldt; Simone Weyand; Georgia Kefala; Manfred S Weiss
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2008-08-29

3.  Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of the regulatory domain of aspartokinase (Rv3709c) from Mycobacterium tuberculosis.

Authors:  Linda Schuldt; Ruth Suchowersky; Katharina Veith; Jochen Mueller-Dieckmann; Manfred S Weiss
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2011-02-25

4.  Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of succinyl-diaminopimelate desuccinylase (Rv1202, DapE) from Mycobacterium tuberculosis.

Authors:  Linda Reinhard; Jochen Mueller-Dieckmann; Manfred S Weiss
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2012-08-31
  4 in total

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