Literature DB >> 1563047

Heterologous transformation of Mucor circinelloides with the Phycomyces blakesleeanus leu1 gene.

E A Iturriaga1, J M Díaz-Mínguez, E P Benito, M I Alvarez, A P Eslava.   

Abstract

The leu1 gene of Phycomyces blakesleeanus was isolated within a HindIII-HindIII genomic DNA fragment by heterologous hybridization screening of a cosmid library, making use of the Mucor circinelloides leuA gene as a probe. The complete nucleotide sequence of this fragment reveals a single 2070 bp ORF with no introns, which presents at least 68% homology with that of the leuA gene. The P. blakesleeanus leu1 gene has also been expressed in the M. circinelloides mutant R7B (leu-), which was used to isolate the leuA gene by complementation. The homology with other known sequences shows that the leu1 gene encodes the P. blakesleeanus alpha-IPM (isopropylmalate) isomerase.

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Year:  1992        PMID： 1563047     DOI： 10.1007/bf00336844

Source DB:  PubMed          Journal:  Curr Genet        ISSN： 0172-8083            Impact factor:   3.886


  30 in total

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Authors:  B J Graves; P F Johnson; S L McKnight
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2.  Low repetitive DNA content in Aspergillus nidulans.

Authors:  W E Timberlake
Journal:  Science       Date:  1978-12-01       Impact factor: 47.728

3.  Transformation of Phycomyces with a bacterial gene for kanamycin resistance.

Authors:  T Suárez; A P Eslava
Journal:  Mol Gen Genet       Date:  1988-04

4.  Rapid and efficient cosmid cloning.

Authors:  D Ish-Horowicz; J F Burke
Journal:  Nucleic Acids Res       Date:  1981-07-10       Impact factor: 16.971

5.  A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.

Authors:  A P Feinberg; B Vogelstein
Journal:  Anal Biochem       Date:  1983-07-01       Impact factor: 3.365

6.  The isolation of nuclei from the filamentous fungus Aspergillus nidulans.

Authors:  M A Gealt; G Sheir-Neiss; N R Morris
Journal:  J Gen Microbiol       Date:  1976-05

7.  Isolation and molecular analysis of the orotidine-5'-phosphate decarboxylase gene (pyrG) of Phycomyces blakesleeanus.

Authors:  J M Díaz-Mínguez; E A Iturriaga; E P Benito; L M Corrochano; A P Eslava
Journal:  Mol Gen Genet       Date:  1990-11

8.  Yeast LEU1. Repression of mRNA levels by leucine and relationship of 5'-noncoding region to that of LEU2.

Authors:  Y P Hsu; P Schimmel
Journal:  J Biol Chem       Date:  1984-03-25       Impact factor: 5.157

9.  Neomycin resistance as a dominantly selectable marker for transformation of the zygomycete Absidia glauca.

Authors:  J Wöstemeyer; A Burmester; C Weigel
Journal:  Curr Genet       Date:  1987       Impact factor: 3.886

10.  Structural organization of the TRP1 gene of Phycomyces blakesleeanus: implications for evolutionary gene fusion in fungi.

Authors:  H T Choi; J L Revuelta; C Sadhu; M Jayaram
Journal:  Gene       Date:  1988-11-15       Impact factor: 3.688
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  8 in total

1.  Expression of Xanthophyllomyces dendrorhous cytochrome-P450 hydroxylase and reductase in Mucor circinelloides.

Authors:  Árpád Csernetics; Eszter Tóth; Anita Farkas; Gábor Nagy; Ottó Bencsik; Csaba Vágvölgyi; Tamás Papp
Journal:  World J Microbiol Biotechnol       Date:  2014-12-11       Impact factor: 3.312

2.  Two origins for the gene encoding alpha-isopropylmalate synthase in fungi.

Authors:  Erica M Larson; Alexander Idnurm
Journal:  PLoS One       Date:  2010-07-15       Impact factor: 3.240

3.  Expression of the carG gene, encoding geranylgeranyl pyrophosphate synthase, is up-regulated by blue light in Mucor circinelloides.

Authors:  Antonio Velayos; Tamás Papp; Raúl Aguilar-Elena; Mónica Fuentes-Vicente; Arturo P Eslava; Enrique A Iturriaga; María Isabel Alvarez
Journal:  Curr Genet       Date:  2003-02-21       Impact factor: 3.886

4.  Isolation of the facA (acetyl-CoA synthetase) gene of Phycomyces blakesleeanus.

Authors:  V Garre; F J Murillo; S Torres-Martínez
Journal:  Mol Gen Genet       Date:  1994-08-02

5.  Development of a system for integrative and stable transformation of the zygomycete Rhizopus oryzae by Agrobacterium-mediated DNA transfer.

Authors:  C B Michielse; K Salim; P Ragas; A F J Ram; B Kudla; B Jarry; P J Punt; C A M J J van den Hondel
Journal:  Mol Genet Genomics       Date:  2004-04-06       Impact factor: 3.291

6.  Isolation, characterization and transformation, by autonomous replication, of Mucor circinelloides OMPdecase-deficient mutants.

Authors:  E P Benito; V Campuzano; M A Lŏpez-Matas; J I De Vicente; A P Eslava
Journal:  Mol Gen Genet       Date:  1995-07-28

7.  Agrobacterium tumefaciens-mediated transformation of Mucor circinelloides.

Authors:  I Nyilasi; K Acs; T Papp; E Nagy; C Vágvölgyi
Journal:  Folia Microbiol (Praha)       Date:  2005       Impact factor: 2.629

8.  Functional analysis of the Phycomyces carRA gene encoding the enzymes phytoene synthase and lycopene cyclase.

Authors:  Catalina Sanz; Antonio Velayos; María Isabel Álvarez; Ernesto P Benito; Arturo P Eslava
Journal:  PLoS One       Date:  2011-08-09       Impact factor: 3.240
  8 in total

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