Literature DB >> 16400184

The akuB(KU80) mutant deficient for nonhomologous end joining is a powerful tool for analyzing pathogenicity in Aspergillus fumigatus.

Márcia Eliana da Silva Ferreira1, Marcia R V Z Kress, Marcela Savoldi, Maria Helena S Goldman, Albert Härtl, Thorsten Heinekamp, Axel A Brakhage, Gustavo H Goldman.   

Abstract

To increase the frequency of homologous recombination, we inactivated the KU80 homologue in Aspergillus fumigatus (named akuB(KU80)). Homologous integration reached about 80% for both calcineurin A (calA) and polyketide synthase pksP (alb1) genes in the akuB(KU80) mutant to 3 and 5%, respectively, when using a wild-type A. fumigatus strain. Deletion of akuB(KU80) had no influence on pathogenicity in a low-dose murine infection model.

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Year:  2006        PMID： 16400184      PMCID： PMC1360264          DOI： 10.1128/EC.5.1.207-211.2006

Source DB:  PubMed          Journal:  Eukaryot Cell        ISSN： 1535-9786


  21 in total

1.  Applications of the long and accurate polymerase chain reaction method in yeast molecular biology: direct sequencing of the amplified DNA and its introduction into yeast.

Authors:  Y Takita; M Takahara; S Nogami; Y Anraku; Y Ohya
Journal:  Yeast       Date:  1997-06-30       Impact factor: 3.239

2.  Selection of multiple disruption events in Aspergillus fumigatus using the orotidine-5'-decarboxylase gene, pyrG, as a unique transformation marker.

Authors:  C d'Enfert
Journal:  Curr Genet       Date:  1996-06       Impact factor: 3.886

3.  The developmentally regulated alb1 gene of Aspergillus fumigatus: its role in modulation of conidial morphology and virulence.

Authors:  H F Tsai; Y C Chang; R G Washburn; M H Wheeler; K J Kwon-Chung
Journal:  J Bacteriol       Date:  1998-06       Impact factor: 3.490

4.  Identification of a polyketide synthase gene (pksP) of Aspergillus fumigatus involved in conidial pigment biosynthesis and virulence.

Authors:  K Langfelder; B Jahn; H Gehringer; A Schmidt; G Wanner; A A Brakhage
Journal:  Med Microbiol Immunol       Date:  1998-10       Impact factor: 3.402

5.  Genetic and physiological regulation of non-homologous end-joining in mammalian cells.

Authors:  Akira Tachibana
Journal:  Adv Biophys       Date:  2004

6.  Rapid production of gene replacement constructs and generation of a green fluorescent protein-tagged centromeric marker in Aspergillus nidulans.

Authors:  Lin Yang; Leena Ukil; Aysha Osmani; Francis Nahm; Jonathan Davies; Colin P C De Souza; Xiaowei Dou; Ariadna Perez-Balaguer; Stephen A Osmani
Journal:  Eukaryot Cell       Date:  2004-10

7.  Deletion of the Aspergillus fumigatus lysine biosynthesis gene lysF encoding homoaconitase leads to attenuated virulence in a low-dose mouse infection model of invasive aspergillosis.

Authors:  Burghard Liebmann; Thomas W Mühleisen; Meike Müller; Matthias Hecht; Gerhard Weidner; Armin Braun; Matthias Brock; Axel A Brakhage
Journal:  Arch Microbiol       Date:  2004-03-30       Impact factor: 2.552

8.  New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae.

Authors:  A Wach; A Brachat; R Pöhlmann; P Philippsen
Journal:  Yeast       Date:  1994-12       Impact factor: 3.239

9.  Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus.

Authors:  William C Nierman; Arnab Pain; Michael J Anderson; Jennifer R Wortman; H Stanley Kim; Javier Arroyo; Matthew Berriman; Keietsu Abe; David B Archer; Clara Bermejo; Joan Bennett; Paul Bowyer; Dan Chen; Matthew Collins; Richard Coulsen; Robert Davies; Paul S Dyer; Mark Farman; Nadia Fedorova; Natalie Fedorova; Tamara V Feldblyum; Reinhard Fischer; Nigel Fosker; Audrey Fraser; Jose L García; Maria J García; Arlette Goble; Gustavo H Goldman; Katsuya Gomi; Sam Griffith-Jones; Ryan Gwilliam; Brian Haas; Hubertus Haas; David Harris; H Horiuchi; Jiaqi Huang; Sean Humphray; Javier Jiménez; Nancy Keller; Hoda Khouri; Katsuhiko Kitamoto; Tetsuo Kobayashi; Sven Konzack; Resham Kulkarni; Toshitaka Kumagai; Anne Lafon; Anne Lafton; Jean-Paul Latgé; Weixi Li; Angela Lord; Charles Lu; William H Majoros; Gregory S May; Bruce L Miller; Yasmin Mohamoud; Maria Molina; Michel Monod; Isabelle Mouyna; Stephanie Mulligan; Lee Murphy; Susan O'Neil; Ian Paulsen; Miguel A Peñalva; Mihaela Pertea; Claire Price; Bethan L Pritchard; Michael A Quail; Ester Rabbinowitsch; Neil Rawlins; Marie-Adele Rajandream; Utz Reichard; Hubert Renauld; Geoffrey D Robson; Santiago Rodriguez de Córdoba; Jose M Rodríguez-Peña; Catherine M Ronning; Simon Rutter; Steven L Salzberg; Miguel Sanchez; Juan C Sánchez-Ferrero; David Saunders; Kathy Seeger; Rob Squares; Steven Squares; Michio Takeuchi; Fredj Tekaia; Geoffrey Turner; Carlos R Vazquez de Aldana; Janice Weidman; Owen White; John Woodward; Jae-Hyuk Yu; Claire Fraser; James E Galagan; Kiyoshi Asai; Masayuki Machida; Neil Hall; Bart Barrell; David W Denning
Journal:  Nature       Date:  2005-12-22       Impact factor: 49.962

10.  Regulation of the mRNA levels of nimA, a gene required for the G2-M transition in Aspergillus nidulans.

Authors:  S A Osmani; G S May; N R Morris
Journal:  J Cell Biol       Date:  1987-06       Impact factor: 10.539
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  186 in total

1.  Fungal ligands released by innate immune effectors promote inflammasome activation during Aspergillus fumigatus infection.

Authors:  Benoit Briard; Rajendra Karki; R K Subbarao Malireddi; Anannya Bhattacharya; David E Place; Jayadev Mavuluri; Jennifer L Peters; Peter Vogel; Masahiro Yamamoto; Thirumala-Devi Kanneganti
Journal:  Nat Microbiol       Date:  2018-12-03       Impact factor: 17.745

2.  α1,3 glucans are dispensable in Aspergillus fumigatus.

Authors:  Christine Henry; Jean-Paul Latgé; Anne Beauvais
Journal:  Eukaryot Cell       Date:  2011-11-04

3.  Validation of a self-excising marker in the human pathogen Aspergillus fumigatus by employing the beta-rec/six site-specific recombination system.

Authors:  Thomas Hartmann; Michaela Dümig; Basem M Jaber; Edyta Szewczyk; Patrick Olbermann; Joachim Morschhäuser; Sven Krappmann
Journal:  Appl Environ Microbiol       Date:  2010-07-23       Impact factor: 4.792

4.  Profiling the Aspergillus fumigatus proteome in response to caspofungin.

Authors:  Steven E Cagas; Mohit Raja Jain; Hong Li; David S Perlin
Journal:  Antimicrob Agents Chemother       Date:  2010-10-25       Impact factor: 5.191

5.  Development of the CRISPR/Cas9 System for Targeted Gene Disruption in Aspergillus fumigatus.

Authors:  Kevin K Fuller; Shan Chen; Jennifer J Loros; Jay C Dunlap
Journal:  Eukaryot Cell       Date:  2015-08-28

6.  An efficient fungal RNA-silencing system using the DsRed reporter gene.

Authors:  Danielle Janus; Birgit Hoff; Eckhard Hofmann; Ulrich Kück
Journal:  Appl Environ Microbiol       Date:  2006-12-01       Impact factor: 4.792

7.  Nonhomologous chromosomal integration of foreign DNA is completely dependent on MUS-53 (human Lig4 homolog) in Neurospora.

Authors:  Kazuma Ishibashi; Keiichiro Suzuki; Yoshinori Ando; Chihiro Takakura; Hirokazu Inoue
Journal:  Proc Natl Acad Sci U S A       Date:  2006-09-26       Impact factor: 11.205

Review 8.  Aspergillus fumigatus: principles of pathogenesis and host defense.

Authors:  Tobias M Hohl; Marta Feldmesser
Journal:  Eukaryot Cell       Date:  2007-09-21

9.  Transcriptional activation of heat shock protein 90 mediated via a proximal promoter region as trigger of caspofungin resistance in Aspergillus fumigatus.

Authors:  Frédéric Lamoth; Praveen R Juvvadi; Christopher Gehrke; Yohannes G Asfaw; William J Steinbach
Journal:  J Infect Dis       Date:  2013-10-04       Impact factor: 5.226

10.  Distinct Roles of Myosins in Aspergillus fumigatus Hyphal Growth and Pathogenesis.

Authors:  Hilary Renshaw; José M Vargas-Muñiz; Amber D Richards; Yohannes G Asfaw; Praveen R Juvvadi; William J Steinbach
Journal:  Infect Immun       Date:  2016-04-22       Impact factor: 3.441
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