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Literature DB >> 20656854

Validation of a self-excising marker in the human pathogen Aspergillus fumigatus by employing the beta-rec/six site-specific recombination system.

Thomas Hartmann¹, Michaela Dümig, Basem M Jaber, Edyta Szewczyk, Patrick Olbermann, Joachim Morschhäuser, Sven Krappmann.

Abstract

Recyclable markers based on site-specific recombination allow repetitive gene targeting in filamentous fungi. Here we describe for the first time functionality of the bacterial recombination system employing beta serine recombinase acting on six recognition sequences (beta-rec/six) in a fungal host, the human pathogen Aspergillus fumigatus, and its use in establishing a self-excising resistance marker cassette for serial gene replacement.

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Year: 2010 PMID： 20656854 PMCID： PMC2937505 DOI： 10.1128/AEM.00882-10

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

46 in total

1. New insights into host factor requirements for prokaryotic beta-recombinase-mediated reactions in mammalian cells.

Authors: V Diaz; P Servert; I Prieto; M A Gonzalez; C Martinez-A; J C Alonso; A Bernad
Journal: J Biol Chem Date: 2001-01-30 Impact factor: 5.157

2. Plant chromosomal HMGB proteins efficiently promote the bacterial site-specific beta-mediated recombination in vitro and in vivo.

Authors: Christian Stemmer; Silvia Fernández; Gema Lopez; Juan C Alonso; Klaus D Grasser
Journal: Biochemistry Date: 2002-06-18 Impact factor: 3.162

3. A second set of loxP marker cassettes for Cre-mediated multiple gene knockouts in budding yeast.

Authors: U Gueldener; J Heinisch; G J Koehler; D Voss; J H Hegemann
Journal: Nucleic Acids Res Date: 2002-03-15 Impact factor: 16.971

4. Concurrent knock-out of at least 20 transporter genes is required to block uptake of hexoses in Saccharomyces cerevisiae.

Authors: R Wieczorke; S Krampe; T Weierstall; K Freidel; C P Hollenberg; E Boles
Journal: FEBS Lett Date: 1999-12-31 Impact factor: 4.124

5. xylP promoter-based expression system and its use for antisense downregulation of the Penicillium chrysogenum nitrogen regulator NRE.

Authors: I Zadra; B Abt; W Parson; H Haas
Journal: Appl Environ Microbiol Date: 2000-11 Impact factor: 4.792

6. Transformation of Aspergillus sp. and Trichoderma reesei using the pyrithiamine resistance gene (ptrA) of Aspergillus oryzae.

Authors: Takafumi Kubodera; Nobuo Yamashita; Akira Nishimura
Journal: Biosci Biotechnol Biochem Date: 2002-02 Impact factor: 2.043

7. HARO7 encodes chorismate mutase of the methylotrophic yeast Hansenula polymorpha and is derepressed upon methanol utilization.

Authors: S Krappmann; R Pries; G Gellissen; M Hiller; G H Braus
Journal: J Bacteriol Date: 2000-08 Impact factor: 3.490

8. Deletion of Aspergillus nidulans aroC using a novel blaster module that combines ET cloning and marker rescue.

Authors: S Krappmann; G H Braus
Journal: Mol Genet Genomics Date: 2003-01-15 Impact factor: 3.291

9. Pyrithiamine resistance gene (ptrA) of Aspergillus oryzae: cloning, characterization and application as a dominant selectable marker for transformation.

Authors: T Kubodera; N Yamashita; A Nishimura
Journal: Biosci Biotechnol Biochem Date: 2000-07 Impact factor: 2.043

10. The Fungal Genetics Stock Center: a repository for 50 years of fungal genetics research.

Authors: K McCluskey; A Wiest; M Plamann
Journal: J Biosci Date: 2010-03 Impact factor: 1.826

45 in total

1. Chitin synthases with a myosin motor-like domain control the resistance of Aspergillus fumigatus to echinocandins.

Authors: Cristina Jiménez-Ortigosa; Vishukumar Aimanianda; Laetitia Muszkieta; Isabelle Mouyna; David Alsteens; Stéphane Pire; Remi Beau; Sven Krappmann; Anne Beauvais; Yves F Dufrêne; César Roncero; Jean-Paul Latgé
Journal: Antimicrob Agents Chemother Date: 2012-09-10 Impact factor: 5.191

Review 2. Recent advances in the understanding of the Aspergillus fumigatus cell wall.

Authors: Mark J Lee; Donald C Sheppard
Journal: J Microbiol Date: 2016-02-27 Impact factor: 3.422

Review 3. Aspergillus fumigatus and aspergillosis: From basics to clinics.

Authors: A Arastehfar; A Carvalho; J Houbraken; L Lombardi; R Garcia-Rubio; J D Jenks; O Rivero-Menendez; R Aljohani; I D Jacobsen; J Berman; N Osherov; M T Hedayati; M Ilkit; D James-Armstrong; T Gabaldón; J Meletiadis; M Kostrzewa; W Pan; C Lass-Flörl; D S Perlin; M Hoenigl
Journal: Stud Mycol Date: 2021-05-10 Impact factor: 16.097

4. The protein phosphatase PhzA of A. fumigatus is involved in oxidative stress tolerance and fungal virulence.

Authors: Laetitia Muszkieta; Steven de Jesus Carrion; Pauline Robinet; Rémi Beau; Carole Elbim; Eric Pearlman; Jean-Paul Latgé
Journal: Fungal Genet Biol Date: 2014-03-12 Impact factor: 3.495

5. Modified Cre-loxP recombination in Aspergillus oryzae by direct introduction of Cre recombinase for marker gene rescue.

Authors: Osamu Mizutani; Kazuo Masaki; Katsuya Gomi; Haruyuki Iefuji
Journal: Appl Environ Microbiol Date: 2012-04-13 Impact factor: 4.792

6. The Siderophore Transporter Sit1 Determines Susceptibility to the Antifungal VL-2397.

Authors: Anna-Maria Dietl; Matthias Misslinger; Mario M Aguiar; Vasyl Ivashov; David Teis; Joachim Pfister; Clemens Decristoforo; Martin Hermann; Sean M Sullivan; Larry R Smith; Hubertus Haas
Journal: Antimicrob Agents Chemother Date: 2019-09-23 Impact factor: 5.191

7. The volatome of Aspergillus fumigatus.

Authors: C Heddergott; A M Calvo; J P Latgé
Journal: Eukaryot Cell Date: 2014-06-06

8. Overlapping and distinct roles of Aspergillus fumigatus UDP-glucose 4-epimerases in galactose metabolism and the synthesis of galactose-containing cell wall polysaccharides.

Authors: Mark J Lee; Fabrice N Gravelat; Robert P Cerone; Stefanie D Baptista; Paolo V Campoli; Se-In Choe; Ilia Kravtsov; Evgeny Vinogradov; Carole Creuzenet; Hong Liu; Albert M Berghuis; Jean-Paul Latgé; Scott G Filler; Thierry Fontaine; Donald C Sheppard
Journal: J Biol Chem Date: 2013-11-20 Impact factor: 5.157

Review 9. Aspergillus fumigatus and Aspergillosis in 2019.

Authors: Jean-Paul Latgé; Georgios Chamilos
Journal: Clin Microbiol Rev Date: 2019-11-13 Impact factor: 26.132

10. Comprehensive Guide to Extracting and Expressing Fungal Secondary Metabolites with Aspergillus fumigatus as a Case Study.

Authors: Grant Nickles; Isabelle Ludwikoski; Jin Woo Bok; Nancy P Keller
Journal: Curr Protoc Date: 2021-12