Literature DB >> 15470263

Rapid production of gene replacement constructs and generation of a green fluorescent protein-tagged centromeric marker in Aspergillus nidulans.

Lin Yang1, Leena Ukil, Aysha Osmani, Francis Nahm, Jonathan Davies, Colin P C De Souza, Xiaowei Dou, Ariadna Perez-Balaguer, Stephen A Osmani.   

Abstract

A method to rapidly generate gene replacement constructs by fusion PCR is described for Aspergillus nidulans. The utility of the approach is demonstrated by green fluorescent protein (GFP) tagging of A. nidulans ndc80 to visualize centromeres through the cell cycle. The methodology makes possible large-scale GFP tagging, promoter swapping, and deletion analysis of A. nidulans.

Entities:  

Mesh：

Substances：

Year:  2004        PMID： 15470263      PMCID： PMC522605          DOI： 10.1128/EC.3.5.1359-1362.2004

Source DB:  PubMed          Journal:  Eukaryot Cell        ISSN： 1535-9786


  12 in total

1.  A generic protein purification method for protein complex characterization and proteome exploration.

Authors:  G Rigaut; A Shevchenko; B Rutz; M Wilm; M Mann; B Séraphin
Journal:  Nat Biotechnol       Date:  1999-10       Impact factor: 54.908

2.  A rapid method for efficient gene replacement in the filamentous fungus Aspergillus nidulans.

Authors:  M K Chaveroche; J M Ghigo; C d'Enfert
Journal:  Nucleic Acids Res       Date:  2000-11-15       Impact factor: 16.971

3.  PCR-mediated generation of a gene disruption construct without the use of DNA ligase and plasmid vectors.

Authors:  Hidekazu Kuwayama; Shinji Obara; Takahiro Morio; Mariko Katoh; Hideko Urushihara; Yoshimasa Tanaka
Journal:  Nucleic Acids Res       Date:  2002-01-15       Impact factor: 16.971

4.  G1-phase and B-type cyclins exclude the DNA-replication factor Mcm4 from the nucleus.

Authors:  K Labib; J F Diffley; S E Kearsey
Journal:  Nat Cell Biol       Date:  1999-11       Impact factor: 28.824

5.  A monomeric red fluorescent protein.

Authors:  Robert E Campbell; Oded Tour; Amy E Palmer; Paul A Steinbach; Geoffrey S Baird; David A Zacharias; Roger Y Tsien
Journal:  Proc Natl Acad Sci U S A       Date:  2002-06-11       Impact factor: 11.205

6.  Characterization of an inducible expression system in Aspergillus nidulans using alcA and tubulin-coding genes.

Authors:  R B Waring; G S May; N R Morris
Journal:  Gene       Date:  1989-06-30       Impact factor: 3.688

7.  Peptide tags for a dual affinity fusion system.

Authors:  J S Kim; R T Raines
Journal:  Anal Biochem       Date:  1994-05-15       Impact factor: 3.365

8.  Plant-adapted green fluorescent protein is a versatile vital reporter for gene expression, protein localization and mitosis in the filamentous fungus, Aspergillus nidulans.

Authors:  J M Fernández-Abalos; H Fox; C Pitt; B Wells; J H Doonan
Journal:  Mol Microbiol       Date:  1998-01       Impact factor: 3.501

9.  Ribonuclease S-peptide as a carrier in fusion proteins.

Authors:  J S Kim; R T Raines
Journal:  Protein Sci       Date:  1993-03       Impact factor: 6.725

10.  The Ndc80p complex from Saccharomyces cerevisiae contains conserved centromere components and has a function in chromosome segregation.

Authors:  P A Wigge; J V Kilmartin
Journal:  J Cell Biol       Date:  2001-01-22       Impact factor: 10.539
View more
  116 in total

1.  The Polo-like kinase PLKA in Aspergillus nidulans is not essential but plays important roles during vegetative growth and development.

Authors:  Klarita Mogilevsky; Amandeep Glory; Catherine Bachewich
Journal:  Eukaryot Cell       Date:  2011-12-02

2.  The two-component histidine kinase Fhk1 controls stress adaptation and virulence of Fusarium oxysporum.

Authors:  Nicolas Rispail; Antonio Di Pietro
Journal:  Mol Plant Pathol       Date:  2010-05       Impact factor: 5.663

3.  Single-step affinity purification for fungal proteomics.

Authors:  Hui-Lin Liu; Aysha H Osmani; Leena Ukil; Sunghun Son; Sarine Markossian; Kuo-Fang Shen; Meera Govindaraghavan; Archana Varadaraj; Shahr B Hashmi; Colin P De Souza; Stephen A Osmani
Journal:  Eukaryot Cell       Date:  2010-04-02

4.  QIP, a protein that converts duplex siRNA into single strands, is required for meiotic silencing by unpaired DNA.

Authors:  Hua Xiao; William G Alexander; Thomas M Hammond; Erin C Boone; Tony D Perdue; Patricia J Pukkila; Patrick K T Shiu
Journal:  Genetics       Date:  2010-06-15       Impact factor: 4.562

5.  Marker fusion tagging, a new method for production of chromosomally encoded fusion proteins.

Authors:  Julian Lai; Seng Kah Ng; Fang Fang Liu; Rajesh Narhari Patkar; Yanfen Lu; Jing Ru Chan; Angayarkanni Suresh; Naweed Naqvi; Gregory Jedd
Journal:  Eukaryot Cell       Date:  2010-03-26

6.  The Aspergillus nidulans Pbp1 homolog is required for normal sexual development and secondary metabolism.

Authors:  Alexandra A Soukup; Gregory J Fischer; Jerry Luo; Nancy P Keller
Journal:  Fungal Genet Biol       Date:  2017-01-09       Impact factor: 3.495

7.  Nuclear export of the transcription factor NirA is a regulatory checkpoint for nitrate induction in Aspergillus nidulans.

Authors:  Andreas Bernreiter; Ana Ramon; Javier Fernández-Martínez; Harald Berger; Lidia Araújo-Bazan; Eduardo A Espeso; Robert Pachlinger; Andreas Gallmetzer; Ingund Anderl; Claudio Scazzocchio; Joseph Strauss
Journal:  Mol Cell Biol       Date:  2006-11-20       Impact factor: 4.272

8.  Functional characterization of Aspergillus nidulans homologues of Saccharomyces cerevisiae Spa2 and Bud6.

Authors:  Aleksandra Virag; Steven D Harris
Journal:  Eukaryot Cell       Date:  2006-06

9.  Distinct ceramide synthases regulate polarized growth in the filamentous fungus Aspergillus nidulans.

Authors:  Shaojie Li; Liangcheng Du; Gary Yuen; Steven D Harris
Journal:  Mol Biol Cell       Date:  2006-01-04       Impact factor: 4.138

10.  A high-throughput gene knockout procedure for Neurospora reveals functions for multiple transcription factors.

Authors:  Hildur V Colot; Gyungsoon Park; Gloria E Turner; Carol Ringelberg; Christopher M Crew; Liubov Litvinkova; Richard L Weiss; Katherine A Borkovich; Jay C Dunlap
Journal:  Proc Natl Acad Sci U S A       Date:  2006-06-26       Impact factor: 11.205
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.