Literature DB >> 16347811

Ethanol extraction requirement for purification of protein labeled with [h]leucine in aquatic bacterial production studies.

R J Wicks1, R D Robarts.   

Abstract

The trichloroacetic acid (TCA)-insoluble fraction of water column bacteria labeled with [H]leucine contained an ethanol-soluble fraction accounting for up to 44% of the label. A component of the ethanol-soluble fraction is [H]leucine. Labeled-protein purification requires an ethanol wash step. Cold TCA can replace hot TCA for precipitation of labeled proteins.

Entities:  

Year:  1988        PMID： 16347811      PMCID： PMC204452          DOI： 10.1128/aem.54.12.3191-3193.1988

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  4 in total

1.  Estimating bacterial production in marine waters from the simultaneous incorporation of thymidine and leucine.

Authors:  G Chin-Leo; D L Kirchman
Journal:  Appl Environ Microbiol       Date:  1988-08       Impact factor: 4.792

2.  Spatial and Temporal Variations in Bacterial Macromolecule Labeling with [methyl-H]Thymidine in a Hypertrophic Lake.

Authors:  R D Robarts; R J Wicks; L M Sephton
Journal:  Appl Environ Microbiol       Date:  1986-12       Impact factor: 4.792

3.  Mechanisms of heat damage in proteins. 8. The role of sucrose in the susceptibility of protein foods to heat damage.

Authors:  R F Hurrell; K J Carpenter
Journal:  Br J Nutr       Date:  1977-09       Impact factor: 3.718

4.  Leucine incorporation and its potential as a measure of protein synthesis by bacteria in natural aquatic systems.

Authors:  D Kirchman; E K'nees; R Hodson
Journal:  Appl Environ Microbiol       Date:  1985-03       Impact factor: 4.792
  4 in total
  4 in total

1.  Incorporation of [h]leucine and [h]valine into protein of freshwater bacteria: uptake kinetics and intracellular isotope dilution.

Authors:  N O Jørgensen
Journal:  Appl Environ Microbiol       Date:  1992-11       Impact factor: 4.792

2.  Impacts of tetracycline on planktonic bacterial production in prairie aquatic systems.

Authors:  Brij Verma; Richard D Robarts; John V Headley
Journal:  Microb Ecol       Date:  2007-01-31       Impact factor: 4.552

3.  Bacterioplankton cell growth and macromolecular synthesis in seawater cultures during the North Atlantic Spring Phytoplankton Bloom, May, 1989.

Authors:  H W Ducklow; D L Kirchman; H L Quinby
Journal:  Microb Ecol       Date:  1992-09       Impact factor: 4.552

4.  Bacterioplankton Production Determined by DNA Synthesis, Protein Synthesis, and Frequency of Dividing Cells in Tuamotu Atoll Lagoons and Surrounding Ocean

Authors: 
Journal:  Microb Ecol       Date:  1996-07       Impact factor: 4.552
  4 in total

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