Literature DB >> 16347424

Determining [H]Thymidine Incorporation into Bacterioplankton DNA: Improvement of the Method by DNase Treatment.

P Servais1, J Martinez, G Billen, J Vives-Rego.   

Abstract

Determination of [H] thymidine incorporation into bacterial DNA versus other macromolecules is usually achieved by NaOH and hot trichloroacetic acid hydrolysis. This procedure was found not to be specific enough. An alternative method founded on DNase treatment is proposed. Under the new method, the fraction of thymidine incorporated into DNA ranged from 10 to 83%.

Entities:  

Year:  1987        PMID： 16347424      PMCID： PMC204039          DOI： 10.1128/aem.53.8.1977-1979.1987

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  10 in total

1.  Measurements of diel rates of bacterial secondary production in aquatic environments.

Authors:  B Riemann; M Søndergaard
Journal:  Appl Environ Microbiol       Date:  1984-04       Impact factor: 4.792

2.  Simultaneous rates of ribonucleic Acid and deoxyribonucleic Acid syntheses for estimating growth and cell division of aquatic microbial communities.

Authors:  D M Karl
Journal:  Appl Environ Microbiol       Date:  1981-11       Impact factor: 4.792

3.  Primary and bacterial production in two dimictic indiana lakes.

Authors:  C R Lovell; A Konopka
Journal:  Appl Environ Microbiol       Date:  1985-03       Impact factor: 4.792

4.  Assessing phytoplankton and bacterioplankton production during early spring in lake erken, sweden.

Authors:  R T Bell; J Kuparinen
Journal:  Appl Environ Microbiol       Date:  1984-12       Impact factor: 4.792

5.  [Chemical reactions in the course of hydrolysis prior to Feulgen's reaction].

Authors:  R THOMAS
Journal:  Bull Soc Chim Biol (Paris)       Date:  1950

6.  Selected nucleic Acid precursors in studies of aquatic microbial ecology.

Authors:  D M Karl
Journal:  Appl Environ Microbiol       Date:  1982-10       Impact factor: 4.792

7.  Fluorometric determination of DNA in aquatic microorganisms by use of hoechst 33258.

Authors:  J H Paul; B Myers
Journal:  Appl Environ Microbiol       Date:  1982-06       Impact factor: 4.792

8.  Bacterioplankton secondary production estimates for coastal waters of british columbia, antarctica, and california.

Authors:  J A Fuhrman; F Azam
Journal:  Appl Environ Microbiol       Date:  1980-06       Impact factor: 4.792

Review 9.  The determination of nucleic acids.

Authors:  H N Munro
Journal:  Methods Biochem Anal       Date:  1966

10.  [Arrhythmias and symptoms during long-term ECG in ambulatory patients].

Authors:  E Fischer; F Graf; S Carli
Journal:  Schweiz Rundsch Med Prax       Date:  1984-01-17
  10 in total
  15 in total

1.  Assessment of [h]thymidine incorporation into DNA as a method to determine bacterial productivity in stream bed sediments.

Authors:  L A Kaplan; T L Bott; J K Bielicki
Journal:  Appl Environ Microbiol       Date:  1992-11       Impact factor: 4.792

2.  Estimation ofEscherichia coli mortality in seawater by the decrease in(3)H-label and electron transport system activity.

Authors:  J Martinez; J Garcia-Lara; J Vives-Rego
Journal:  Microb Ecol       Date:  1989-05       Impact factor: 4.552

3.  Benthic bacterial biomass and production in the Hudson River estuary.

Authors:  H K Austin; S E Findlay
Journal:  Microb Ecol       Date:  1989-09       Impact factor: 4.552

4.  Experimental evaluation of conversion factors for the [h]thymidine incorporation assay of bacterial secondary productivity.

Authors:  M F Coveney; R G Wetzel
Journal:  Appl Environ Microbiol       Date:  1988-08       Impact factor: 4.792

5.  DNA Synthesis and Tritiated Thymidine Incorporation by Heterotrophic Freshwater Bacteria in Continuous Culture.

Authors:  Frank M Ellenbroek; Thomas E Cappenberg
Journal:  Appl Environ Microbiol       Date:  1991-06       Impact factor: 4.792

6.  Protozoan grazing and bacterial production in stratified lake vechten estimated with fluorescently labeled bacteria and by thymidine incorporation.

Authors:  J Bloem; F M Ellenbroek; M J Bär-Gilissen; T E Cappenberg
Journal:  Appl Environ Microbiol       Date:  1989-07       Impact factor: 4.792

7.  Catabolism of tritiated thymidine by aquatic microbial communities and incorporation of tritium into RNA and protein.

Authors:  A M Brittain; D M Karl
Journal:  Appl Environ Microbiol       Date:  1990-05       Impact factor: 4.792

8.  Bacterioplankton and organic carbon dynamics in the lower mesohaline chesapeake bay.

Authors:  R B Jonas; J H Tuttle
Journal:  Appl Environ Microbiol       Date:  1990-03       Impact factor: 4.792

9.  Purification of DNA for bacterial productivity estimates.

Authors:  B K Burnison; D J Nuttley
Journal:  Appl Environ Microbiol       Date:  1990-02       Impact factor: 4.792

10.  Estimates of bacterial productivity in marine sediments and water from a temperate saltmarsh lagoon.

Authors:  B J Tibbles; C L Davis; J M Harris; M I Lucas
Journal:  Microb Ecol       Date:  1992-05       Impact factor: 4.552
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