Literature DB >> 2154943

Purification of DNA for bacterial productivity estimates.

B K Burnison1, D J Nuttley.   

Abstract

[methyl-3H]thymidine-labeled DNA from natural populations of aquatic bacteria was completely separated from RNA and protein by hydroxylapatite chromatography. The procedure was validated by monitoring increases in Escherichia coli cell count, A550, DNA concentration, and thymidine incorporation into DNA isolated by the proposed technique. The procedure can be used in the field and does not rely on the use of acid-base hydrolysis or volatile organic solvents.

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Year:  1990        PMID： 2154943      PMCID： PMC183345          DOI： 10.1128/aem.56.2.362-365.1990

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  16 in total

1.  Primary and bacterial production in two dimictic indiana lakes.

Authors:  C R Lovell; A Konopka
Journal:  Appl Environ Microbiol       Date:  1985-03       Impact factor: 4.792

2.  Experimental evaluation of conversion factors for the [h]thymidine incorporation assay of bacterial secondary productivity.

Authors:  M F Coveney; R G Wetzel
Journal:  Appl Environ Microbiol       Date:  1988-08       Impact factor: 4.792

3.  Estimating Bacterioplankton Production by Measuring [H]thymidine Incorporation in a Eutrophic Swedish Lake.

Authors:  R T Bell; G M Ahlgren; I Ahlgren
Journal:  Appl Environ Microbiol       Date:  1983-06       Impact factor: 4.792

4.  Spatial and Temporal Variations in Bacterial Macromolecule Labeling with [methyl-H]Thymidine in a Hypertrophic Lake.

Authors:  R D Robarts; R J Wicks; L M Sephton
Journal:  Appl Environ Microbiol       Date:  1986-12       Impact factor: 4.792

5.  Fluorometric determination of DNA in aquatic microorganisms by use of hoechst 33258.

Authors:  J H Paul; B Myers
Journal:  Appl Environ Microbiol       Date:  1982-06       Impact factor: 4.792

6.  Rapid isolation of mouse DNA from cells in tissue culture.

Authors:  W Meinke; D A Goldstein; M R Hall
Journal:  Anal Biochem       Date:  1974-03       Impact factor: 3.365

7.  Fluorometric determination of the DNA concentration in municipal drinking water.

Authors:  W F McCoy; B H Olson
Journal:  Appl Environ Microbiol       Date:  1985-04       Impact factor: 4.792

8.  [Arrhythmias and symptoms during long-term ECG in ambulatory patients].

Authors:  E Fischer; F Graf; S Carli
Journal:  Schweiz Rundsch Med Prax       Date:  1984-01-17

9.  Isolation and quantitation of DNA-bound benzo(a)pyrene metabolites: comparison of hydroxyapatite and precipitation procedures.

Authors:  P I Adriaenssens; C J Bixler; M W Anderson
Journal:  Anal Biochem       Date:  1982-06       Impact factor: 3.365

10.  Validity of the tritiated thymidine method for estimating bacterial growth rates: measurement of isotope dilution during DNA synthesis.

Authors:  P C Pollard; D J Moriarty
Journal:  Appl Environ Microbiol       Date:  1984-12       Impact factor: 4.792
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