Literature DB >> 16346888

Genetic Recombination and Transformation in Protoplasts of Thermomonospora fusca.

K A Pidcock1, B S Montenecourt, J A Sands.   

Abstract

Protoplasts were produced from the thermophilic actinomycete Thermomonospora fusca and were regenerated to 0.1% of the direct count on regeneration agar. Recombination after protoplast fusion was demonstrated with drug-resistant mutants of T. fusca YX. A single thiostrepton-resistant colony was isolated after transformation of T. fusca YX with the streptomycete vector pIJ702, providing the first evidence for transformation in the genus Thermomonospora and suggesting that some mesophilic streptomycete genes can be expressed in thermophilic actinomycetes. Of 20 thermophilic actinomycete strains isolated from self-heated composts, 3 were found to harbor native plasmid DNA, providing potential sequences for the development of Thermomonospora-Streptomyces shuttle vectors.

Entities:  

Year:  1985        PMID： 16346888      PMCID： PMC238691          DOI： 10.1128/aem.50.3.693-695.1985

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  9 in total

1.  A new method for the purification and identification of covalently closed circular DNA molcules.

Authors:  M Zasloff; G D Ginder; G Felsenfeld
Journal:  Nucleic Acids Res       Date:  1978-04       Impact factor: 16.971

Review 2.  Actinomycetes in soils, composts and fodders.

Authors:  J Lacey
Journal:  Soc Appl Bacteriol Symp Ser       Date:  1973-01

3.  Novel method for selective isolation of actinomycetes.

Authors:  C F Hirsch; D L Christensen
Journal:  Appl Environ Microbiol       Date:  1983-10       Impact factor: 4.792

Review 4.  Gene cloning in Streptomyces.

Authors:  K F Chater; D A Hopwood; T Kieser; C J Thompson
Journal:  Curr Top Microbiol Immunol       Date:  1982       Impact factor: 4.291

5.  DNA cloning in Streptomyces: resistance genes from antibiotic-producing species.

Authors:  C J Thompson; J M Ward; D A Hopwood
Journal:  Nature       Date:  1980-07-31       Impact factor: 49.962

6.  Cellulase biosynthesis in a catabolite repression-resistant mutant of Thermomonospora curvata.

Authors:  G Fennington; D Neubauer; F Stutzenberger
Journal:  Appl Environ Microbiol       Date:  1984-01       Impact factor: 4.792

7.  Mechanism of resistance to thiostrepton in the producing-organism Streptomyces azureus.

Authors:  E Cundliffe
Journal:  Nature       Date:  1978-04-27       Impact factor: 49.962

8.  Formation and reversion of Streptomycete protoplasts: cultural condition and morphological study.

Authors:  M Okanishi; K Suzuki; H Umezawa
Journal:  J Gen Microbiol       Date:  1974-02

9.  Cloning and expression of the tyrosinase gene from Streptomyces antibioticus in Streptomyces lividans.

Authors:  E Katz; C J Thompson; D A Hopwood
Journal:  J Gen Microbiol       Date:  1983-09
  9 in total
  7 in total

1.  Transduction of plasmid DNA in Streptomyces spp. and related genera by bacteriophage FP43.

Authors:  M A McHenney; R H Baltz
Journal:  J Bacteriol       Date:  1988-05       Impact factor: 3.490

2.  Preliminary characterization of bacteriophages infecting the thermophilic actinomycete thermomonospora.

Authors:  H M Lawrence; H Merivuori; J A Sands; K A Pidcock
Journal:  Appl Environ Microbiol       Date:  1986-10       Impact factor: 4.792

3.  Expression of a Thermomonospora fusca Cellulase Gene in Streptomyces lividans and Bacillus subtilis.

Authors:  G S Ghangas; D B Wilson
Journal:  Appl Environ Microbiol       Date:  1987-07       Impact factor: 4.792

4.  A novel, highly efficient gene-cloning system for Micromonospora strains.

Authors:  M Hasegawa; T Dairi; T Ohta; E Hashimoto
Journal:  J Bacteriol       Date:  1991-11       Impact factor: 3.490

5.  Development and application of a PCR-targeted gene disruption method for studying CelR function in Thermobifida fusca.

Authors:  Yu Deng; Stephen S Fong
Journal:  Appl Environ Microbiol       Date:  2010-01-22       Impact factor: 4.792

6.  Efficient plasmid transformation of the beta-lactam producer Streptomyces clavuligerus.

Authors:  M Garcia-Dominguez; J F Martin; B Mahro; A L Demain; P Liras
Journal:  Appl Environ Microbiol       Date:  1987-06       Impact factor: 4.792

7.  Efficient transformation of Amycolatopsis orientalis (Nocardia orientalis) protoplasts by Streptomyces plasmids.

Authors:  P Matsushima; M A McHenney; R H Baltz
Journal:  J Bacteriol       Date:  1987-05       Impact factor: 3.490
  7 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.